{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T18:43:57Z","timestamp":1770576237552,"version":"3.49.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2011,2,1]],"date-time":"2011-02-01T00:00:00Z","timestamp":1296518400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Surveillance for\n            <jats:italic>Plasmodium falciparum<\/jats:italic>\n            drug resistance mutations is becoming an established tool for assessing antimalarial treatment effectiveness. We used an extended version of a high-throughput post-PCR multiplexed ligase detection reaction fluorescent microsphere assay (LDR-FMA) to detect single-nucleotide\n            <jats:italic>P. falciparum<\/jats:italic>\n            drug resistance polymorphisms in 402 isolates from children in Papua New Guinea (PNG) participating in an antimalarial treatment trial. There was a fixation of\n            <jats:italic>P. falciparum crt<\/jats:italic>\n            (\n            <jats:italic>pfcrt<\/jats:italic>\n            ) K76T,\n            <jats:italic>pfdhfr<\/jats:italic>\n            C59R and S108N, and\n            <jats:italic>pfmdr1<\/jats:italic>\n            mutations (92%, 93%, 95%, and 91%, respectively). Multiple mutations were frequent. Eighty-eight percent of isolates possessed a quintuple mutation (underlined),\n            <jats:underline>S<\/jats:underline>\n            VMN\n            <jats:underline>T<\/jats:underline>\n            , N\n            <jats:underline>RN<\/jats:underline>\n            I, KAA, and\n            <jats:underline>Y<\/jats:underline>\n            YSND, in codons 72 to 76 for\n            <jats:italic>pfcrt<\/jats:italic>\n            ; 51, 59, 108, and 164 for\n            <jats:italic>pfdhfr<\/jats:italic>\n            ; 540, 581, and 613 for\n            <jats:italic>pfdhps<\/jats:italic>\n            ; and 86, 184, 1034, 1042, and 1246 for\n            <jats:italic>pfmdr1<\/jats:italic>\n            , and four of these carried the K540E\n            <jats:italic>pfdhps<\/jats:italic>\n            allele. The\n            <jats:italic>pfmdr1<\/jats:italic>\n            D1246Y mutation was associated with PCR-corrected day 42\n            <jats:italic>in vivo<\/jats:italic>\n            treatment failure in children allocated piperaquine-dihydroartemisinin (\n            <jats:italic>P<\/jats:italic>\n            = 0.004). Although the\n            <jats:italic>pfmdr1<\/jats:italic>\n            NFSDD haplotype was found in only four isolates, it has been associated with artemether-lumefantrine treatment failure in Africa. LDR-FMA allows the large-scale assessment of resistance-associated single-nucleotide polymorphisms (SNPs). Our findings reflect previous heavy 4-aminoquinoline\/sulfadoxine-pyrimethamine use in PNG. Since artemether-lumefantrine and piperaquine-dihydroartemisinin will become first- and second-line treatments, respectively, the monitoring of\n            <jats:italic>pfmdr1<\/jats:italic>\n            SNPs appears to be a high priority.\n          <\/jats:p>","DOI":"10.1128\/aac.00939-10","type":"journal-article","created":{"date-parts":[[2010,11,16]],"date-time":"2010-11-16T04:14:55Z","timestamp":1289880895000},"page":"798-805","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Molecular Assessment of\n            <i>Plasmodium falciparum<\/i>\n            Resistance to Antimalarial Drugs in Papua New Guinea Using an Extended Ligase Detection Reaction Fluorescent Microsphere Assay"],"prefix":"10.1128","volume":"55","author":[{"given":"Rina P. M.","family":"Wong","sequence":"first","affiliation":[{"name":"School of Medicine and Pharmacology, Fremantle Unit, University of Western Australia, Nedlands, Western Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harin","family":"Karunajeewa","sequence":"additional","affiliation":[{"name":"School of Medicine and Pharmacology, Fremantle Unit, University of Western Australia, Nedlands, Western Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivo","family":"Mueller","sequence":"additional","affiliation":[{"name":"Papua New Guinea Institute of Medical Research, Goroka, Papua New Guinea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Siba","sequence":"additional","affiliation":[{"name":"Papua New Guinea Institute of Medical Research, Goroka, Papua New Guinea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter A.","family":"Zimmerman","sequence":"additional","affiliation":[{"name":"Center for Global Health and Diseases, Case Western Reserve University, Cleveland, Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy M. E.","family":"Davis","sequence":"additional","affiliation":[{"name":"School of Medicine and Pharmacology, Fremantle Unit, University of Western Australia, Nedlands, Western Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2005.72.155"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006762"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2002.67.392"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01683-06"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2004.70.251"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.3.1143"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.23.9109"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01178-07"},{"key":"e_1_3_2_10_2","first-page":"57","volume":"11","year":"2010","unstructured":"DaRe, J. 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