{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T02:35:42Z","timestamp":1790562942704,"version":"4.1.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T00:00:00Z","timestamp":1728345600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T00:00:00Z","timestamp":1728345600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32060018"],"award-info":[{"award-number":["32060018"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2024,10,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Helicobacter pylori<\/jats:italic>\n              (\n              <jats:italic>H. pylori<\/jats:italic>\n              ) is closely associated with the diseases such as gastric sinusitis, peptic ulcers, and gastric adenocarcinoma. Its drug resistance is very severe, and new antibiotics are urgently needed. Nine comfrey compounds were screened by antimicrobial susceptibility testing, among which deoxyshikonin had the best inhibitory effect, with a minimum inhibitory concentration (MIC) of 0.5\u20131 \u00b5g\/mL. In addition, deoxyshikonin also has a good antibacterial effect in an acidic environment, it is highly safe, and\n              <jats:italic>H. pylori<\/jats:italic>\n              does not readily develop drug resistance. Through\n              <jats:italic>in vivo<\/jats:italic>\n              experiments, it was proven that deoxyshikonin (7 mg\/kg) had a beneficial therapeutic effect on acute gastritis in mice infected with the multidrug-resistant\n              <jats:italic>H. pylori<\/jats:italic>\n              BS001 strain. After treatment with desoxyshikonin, colonization of\n              <jats:italic>H. pylori<\/jats:italic>\n              in the gastric mucosa of mice was significantly reduced, gastric mucosal damage was repaired, inflammatory factors were reduced, and the treatment effect was better than that of standard triple therapy. Therefore, deoxyshikonin is a promising lead drug to solve the difficulty of drug resistance in\n              <jats:italic>H. pylori<\/jats:italic>\n              , and its antibacterial mechanism may be to destroy the biofilm and cause an oxidation reaction.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.00959-24","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T13:06:49Z","timestamp":1724332009000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Deoxyshikonin: a promising lead drug grass against drug resistance or sensitivity to\n            <i>Helicobacter pylori<\/i>\n            in an acidic environment"],"prefix":"10.1128","volume":"68","author":[{"given":"Jia-yin","family":"Xu","sequence":"first","affiliation":[{"name":"Guangxi Technology Innovation Cooperation Base of Prevention and Control Pathogenic Microbes with Drug Resistance, Youjiang Medical University for Nationalities, Baise, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-hua","family":"Dong","sequence":"additional","affiliation":[{"name":"Guangxi Technology Innovation Cooperation Base of Prevention and Control Pathogenic Microbes with Drug Resistance, Youjiang Medical University for Nationalities, Baise, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-juan","family":"Liao","sequence":"additional","affiliation":[{"name":"Guangxi Technology Innovation Cooperation Base of Prevention and Control Pathogenic Microbes with Drug Resistance, Youjiang Medical University for Nationalities, Baise, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-xian","family":"Yang","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China"},{"name":"Guangxi Zhuang Autonomous Region Engineering Research Center of Clinical Prevention and Control Technology and Leading Drug for Microorganisms with Drug Resistance in Border Ethnic Areas, Baise, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu-yao","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangxi Technology Innovation Cooperation Base of Prevention and Control Pathogenic Microbes with Drug Resistance, Youjiang Medical University for Nationalities, Baise, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Basic Medical Sciences, Wannan Medical College, Wuhu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peipei","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Wujin People\u2019s Hospital affiliated to JiangSu University, Changzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7937-8150","authenticated-orcid":false,"given":"Liang","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangxi Technology Innovation Cooperation Base of Prevention and Control Pathogenic Microbes with Drug Resistance, Youjiang Medical University for Nationalities, Baise, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7409-941X","authenticated-orcid":false,"given":"Ai-xing","family":"Guan","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China"},{"name":"Guangxi Zhuang Autonomous Region Engineering Research Center of Clinical Prevention and Control Technology and Leading Drug for Microorganisms with Drug Resistance in Border Ethnic Areas, Baise, 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