{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:57:44Z","timestamp":1789567064245,"version":"build-2803163510"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T00:00:00Z","timestamp":1704844800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T00:00:00Z","timestamp":1704844800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Ministerio de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","award":["PICT 2019-04338"],"award-info":[{"award-number":["PICT 2019-04338"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2024,1,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Antifungal susceptibility testing (AST) is crucial in clinical settings to guide appropriate therapy. Nevertheless, discrepancies between treatment response and some results still persist, particularly in detecting resistance to amphotericin B (AMB) in\n            <jats:italic>Clavispora<\/jats:italic>\n            (\n            <jats:italic>Candida<\/jats:italic>\n            )\n            <jats:italic>lusitaniae<\/jats:italic>\n            . This study aimed to assess the susceptibility patterns of 48 recent isolates of\n            <jats:italic>C. lusitaniae<\/jats:italic>\n            to 9 antifungal agents and explore the feasibility of using a CLSI reference-based method to identify AMB resistance. Microdilution techniques revealed a wide range of minimal inhibitory concentration (MIC) values for azole antifungals, while echinocandins and AMB exhibited a narrow range of MIC values, with all strains considered wild-type for the tested polyene and echinocandins. However, when agar diffusion (ellipsometry) was employed for AST, certain strains displayed colonies within the inhibition ellipse, indicating potential resistance. Interestingly, these strains did not respond to AMB treatment and were isolated during AMB treatment (breakthrough). Moreover, the evaluation of AMB minimum fungicidal concentrations (MFCs) indicated that only the strains with colonies inside the ellipse had MFC\/MIC ratios \u2265 4, suggesting reduced fungicidal activity. In conclusion, this study confirms the effectiveness of ellipsometry with RPMI-1640 2% glucose agar for detecting AMB resistance in\n            <jats:italic>C. lusitaniae<\/jats:italic>\n            . Additionally, the proposed approach of culturing \u201cclear\u201d wells in the microdilution method can aid in uncovering resistant strains. The findings highlight the importance of appropriate AST methods to guide effective treatment strategies for deep-seated candidiasis caused by\n            <jats:italic>C. lusitaniae<\/jats:italic>\n            . Further collaborative studies are warranted to validate these findings and improve the detection of AMB clinical resistance.\n          <\/jats:p>","DOI":"10.1128\/aac.00968-23","type":"journal-article","created":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T14:01:25Z","timestamp":1702389685000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Antifungal susceptibility testing following the CLSI M27 document, along with the measurement of MFC\/MIC ratio, could be the optimal approach to detect amphotericin B resistance in\n            <i>Clavispora<\/i>\n            (\n            <i>Candida<\/i>\n            )\n            <i>lusitaniae<\/i>\n            . Susceptibility patterns of contemporary isolates of this species"],"prefix":"10.1128","volume":"68","author":[{"given":"Natalia","family":"Sasoni","sequence":"first","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular \u2013 C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda \u2013 Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas \u2013 Universidad Nacional del Litoral , Santa Fe, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) , Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Caracciolo","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda Laboratorio de Micolog\u00eda Hospital Juan P Garrahan , Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mat\u00edas S.","family":"Cabeza","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular \u2013 C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda \u2013 Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas \u2013 Universidad Nacional del Litoral , Santa Fe, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) , Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soledad","family":"Gamarra","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular \u2013 C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda \u2013 Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas \u2013 Universidad Nacional del Litoral , Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Carnovale","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda Laboratorio de Micolog\u00eda Hospital Juan P Garrahan , Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3927-4650","authenticated-orcid":false,"given":"Guillermo","family":"Garcia-Effron","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular \u2013 C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda \u2013 Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas \u2013 Universidad Nacional del Litoral , Santa Fe, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) , Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof3040057"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2021.03.007"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx444"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30316-X"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00069-19"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00248-12"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01253-21"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz568"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02204-15"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.1.339-342.2001"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02512-19"},{"key":"e_1_3_1_13_2","volume-title":"Epidemiological cutoff values for antifungal susceptibility testing, 4th ed","author":"Clinical and Laboratory Strandards Institute CLSI","year":"2022","unstructured":"Clinical and Laboratory Strandards Institute CLSI . 2022. 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