{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T02:21:15Z","timestamp":1791512475346,"version":"4.3.4"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"HHS | National Institutes of Health","award":["P01-AI083214"],"award-info":[{"award-number":["P01-AI083214"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2024,2,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Endogenous transporters protect\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            against antibiotics and also contribute to bacterial defense from environmental toxins. We evaluated the effect of overexpression of four efflux pumps, NorA, NorB, NorC, and Tet38, on\n            <jats:italic>S. aureus<\/jats:italic>\n            survival following exposure to pyocyanin (PYO) of\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            , using a well diffusion assay. We measured the PYO-created inhibition zone and found that only an overexpression of NorA reduced\n            <jats:italic>S. aureus<\/jats:italic>\n            susceptibility to pyocyanin killing. The MIC\n            <jats:sub>PYO<\/jats:sub>\n            of the NorA overexpressor increased threefold compared to that of wild-type RN6390 and was reduced 2.5-fold with reserpine, suggesting that increased NorA efflux caused PYO resistance. The PYO-created inhibition zone of a\n            <jats:italic>\u0394norA<\/jats:italic>\n            mutant was consistently larger than that of a plasmid-borne NorA overexpressor. PYO also produced a modest increase in\n            <jats:italic>norA<\/jats:italic>\n            expression (1.8-fold at 0.25 \u00b5g\/mL PYO) that gradually decreased with increasing PYO concentrations. Well diffusion assays carried out using\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            showed that\n            <jats:italic>\u0394norA<\/jats:italic>\n            mutant was less susceptible to killing by PYO-deficient mutants PA14\n            <jats:italic>\n              <jats:sub>phzM<\/jats:sub>\n            <\/jats:italic>\n            and PA14\n            <jats:italic>\n              <jats:sub>phzS<\/jats:sub>\n            <\/jats:italic>\n            than to killing by PA14. NorA overexpression led to reduced killing by all tested\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            . We evaluated the NorA\u2013PYO interaction using a collection of 22 clinical isolates from adult and pediatric cystic fibrosis (CF) patients, which included both\n            <jats:italic>S. aureus<\/jats:italic>\n            (CF-SA) and\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            (CF-PA). We found that when isolated alone, CF-PA and CF-SA expressed varying levels of PYO and\n            <jats:italic>norA<\/jats:italic>\n            transcripts, but all four CF-PA\/CF-SA pairs isolated concurrently from CF patients produced a low level of PYO and low\n            <jats:italic>norA<\/jats:italic>\n            transcript levels, respectively, suggesting a partial adaptation of the two bacteria in circumstances of persistent co-colonization.\n          <\/jats:p>","DOI":"10.1128\/aac.01001-23","type":"journal-article","created":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T17:30:22Z","timestamp":1705512622000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["NorA efflux pump mediates\n            <i>Staphylococcus aureus<\/i>\n            response to\n            <i>Pseudomonas aeruginosa<\/i>\n            pyocyanin toxicity"],"prefix":"10.1128","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3275-4360","authenticated-orcid":false,"given":"Q. C.","family":"Truong-Bolduc","sequence":"first","affiliation":[{"name":"Infectious Diseases Division and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. M.","family":"Yonker","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Wang","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. G.","family":"Lawton","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. C.","family":"Hooper","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, 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