{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T02:10:09Z","timestamp":1790475009480,"version":"4.1.0"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,11]]},"abstract":"ABSTRACT<\/jats:title>\n \n In invasive candidiasis, there has been an epidemiological shift from\n Candida albicans<\/jats:named-content>\n to non-\n albicans<\/jats:named-content>\n species infections, including infections with\n C. glabrata<\/jats:named-content>\n ,\n C. parapsilosis<\/jats:named-content>\n ,\n C. tropicalis<\/jats:named-content>\n , and\n C. krusei<\/jats:named-content>\n . Although the prevalence of\n C. krusei<\/jats:named-content>\n remains low among yeast infections, its intrinsic resistance to fluconazole raises epidemiological and therapeutic concerns. Echinocandins have\n in vitro<\/jats:italic>\n activity against most\n Candida<\/jats:named-content>\n spp. and are the first-line agents in the treatment of candidemia. Although resistance to echinocandin drugs is still rare, individual cases of\n C. krusei<\/jats:named-content>\n resistance have been reported in recent years, especially with strains that have been under selective pressure. A total of 15\n C. krusei<\/jats:named-content>\n strains, isolated from the blood, urine, and soft tissue of an acute lymphocytic leukemia patient, were analyzed. Strains developed echinocandin resistance during 10 days of caspofungin therapy. The molecular epidemiology of the isolates was investigated using two different typing methods: PCR-based amplification of the species-specific repetitive polymorphic CKRS-1 sequence and multilocus sequence typing. All isolates were genetically related, and the mechanism involved in decreased echinocandin susceptibility was characterized. Clinical resistance was associated with an increase in echinocandin MICs\n in vitro<\/jats:italic>\n and was related to three different mutations in hot spot 1 of the target enzyme Fks1p. Molecular evidence of the rapid acquisition of resistance by different mutations in\n FKS1<\/jats:italic>\n highlights the need to monitor the development of resistance in\n C. krusei<\/jats:named-content>\n infections treated with echinocandin drugs.\n <\/jats:p>","DOI":"10.1128\/aac.01005-15","type":"journal-article","created":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T01:59:49Z","timestamp":1441072789000},"page":"6975-6982","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["Rapid Development of Candida krusei Echinocandin Resistance during Caspofungin Therapy"],"prefix":"10.1128","volume":"59","author":[{"given":"A.","family":"Forastiero","sequence":"first","affiliation":[{"name":"Mycology Reference Laboratory, Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Garcia-Gil","sequence":"additional","affiliation":[{"name":"Mycology Reference Laboratory, Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Rivero-Menendez","sequence":"additional","affiliation":[{"name":"Mycology Reference Laboratory, Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Garcia-Rubio","sequence":"additional","affiliation":[{"name":"Mycology Reference Laboratory, Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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