{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T07:15:32Z","timestamp":1791098132863,"version":"4.1.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T00:00:00Z","timestamp":1728345600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T00:00:00Z","timestamp":1728345600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R21AI153863"],"award-info":[{"award-number":["R21AI153863"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2024,10,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Antimicrobial resistance is extremely common in\n              <jats:italic>Mycoplasma genitalium<\/jats:italic>\n              , a frequent cause of urethritis in men and cervicitis, vaginitis, and pelvic inflammatory disease in women. Treatment of\n              <jats:italic>M. genitalium<\/jats:italic>\n              infections is difficult due to intrinsic and acquired resistance to many antibiotic classes. We undertook a program to identify novel antimicrobials with activity against\n              <jats:italic>M. genitalium<\/jats:italic>\n              from fungal natural products. Extracts of\n              <jats:italic>Ramularia coccinea<\/jats:italic>\n              contained a molecule with potent activity that was subsequently identified as fusidic acid, a fusidane-type antibiotic that has been in clinical use for decades outside the United States. We found that minimum inhibitory concentrations of fusidic acid ranged from 0.31 to 4 \u00b5g\/mL among 17\n              <jats:italic>M<\/jats:italic>\n              .\n              <jats:italic>genitalium<\/jats:italic>\n              strains including laboratory-passaged and low-passage clinical isolates. Time-kill data indicate that bactericidal killing occurs when\n              <jats:italic>M. genitalium<\/jats:italic>\n              is exposed to \u226510 \u00b5g\/mL for 48 h, comparing favorably to serum concentrations obtained from typical loading dose regimens. Resistance to fusidic acid was associated with mutations in\n              <jats:italic>fusA<\/jats:italic>\n              consistent with the known mechanism of action in which fusidic acid inhibits protein synthesis by binding to elongation factor G. Interestingly, no mutants resistant to &gt;10 \u00b5g\/mL fusidic acid were obtained and a resistant strain containing a F435Y mutation in FusA was impaired for growth\n              <jats:italic>in vitro<\/jats:italic>\n              . These data suggest that fusidic acid may be a promising option for the treatment of\n              <jats:italic>M. genitalium<\/jats:italic>\n              infections.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.01006-24","type":"journal-article","created":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T13:02:07Z","timestamp":1724936527000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["The fungal natural product fusidic acid demonstrates potent activity against\n            <i>Mycoplasma genitalium<\/i>"],"prefix":"10.1128","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5109-3794","authenticated-orcid":true,"given":"Gwendolyn E.","family":"Wood","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Woo","family":"Lee","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Duksung Women\u2019s University, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thilini","family":"Peramuna","sequence":"additional","affiliation":[{"name":"Natural Products Discovery Group, Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, Institute for Natural Products Applications and Research Technologies, University of Oklahoma, Norman, Oklahoma, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen L.","family":"Wendt","sequence":"additional","affiliation":[{"name":"Natural Products Discovery Group, Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, Institute for Natural Products Applications and Research Technologies, University of Oklahoma, Norman, Oklahoma, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline M.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8695-6684","authenticated-orcid":true,"given":"Laarni Kendra T.","family":"Aguila","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire L.","family":"Calderon","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert H.","family":"Cichewicz","sequence":"additional","affiliation":[{"name":"Natural Products Discovery Group, Department of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, Institute for Natural Products Applications and Research Technologies, University of Oklahoma, Norman, Oklahoma, 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