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We generated a mutant strain of New Delhi metallo-\u03b2-lactamase-producing\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      with a durlobactam minimum inhibitory concentration of 2 \u00b5g\/mL, representing a 16-fold increase from baseline, by exposing it to increasing concentrations of durlobactam. Resistance was attributed to a point mutation in the\n                      <jats:italic toggle=\"yes\">mrdA<\/jats:italic>\n                      gene, resulting in a V522I substitution in PBP2.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01014-25","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:20:26Z","timestamp":1760106026000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["<i>In vitro<\/i>\n                    evolution of durlobactam resistance in NDM-producing\n                    <i>Escherichia coli<\/i>\n                    due to a single mutation in\n                    <i>mrdA<\/i>\n                    encoding penicillin-binding protein 2"],"prefix":"10.1128","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0967-504X","authenticated-orcid":false,"given":"Christi L.","family":"McElheny","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01an3r305","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Infectious Diseases, University of Pittsburgh School of Medicine","place":["Pittsburgh, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika L.","family":"Butcher","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01an3r305","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Infectious Diseases, University of Pittsburgh School of Medicine","place":["Pittsburgh, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5695-8814","authenticated-orcid":true,"given":"Akito","family":"Kawai","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0232r4451","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Fujita Health University School of Medicine","place":["Toyoake, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7699-5396","authenticated-orcid":true,"given":"Robert M. 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