{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T10:30:59Z","timestamp":1790677859015,"version":"4.1.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Acinetobacter baumannii<\/jats:named-content>\n            is an increasingly problematic pathogen in United States hospitals. Antibiotics that can treat\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. baumannii<\/jats:named-content>\n            are becoming more limited. Little is known about the contributions of penicillin binding proteins (PBPs), the target of \u03b2-lactam antibiotics, to \u03b2-lactam\u2013sulbactam susceptibility and \u03b2-lactam resistance in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. baumannii<\/jats:named-content>\n            . Decreased expression of PBPs as well as loss of binding of \u03b2-lactams to PBPs was previously shown to promote \u03b2-lactam resistance in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. baumannii<\/jats:named-content>\n            . Using an\n            <jats:italic>in vitro<\/jats:italic>\n            assay with a reporter \u03b2-lactam, Bocillin, we determined that the 50% inhibitory concentrations (IC\n            <jats:sub>50<\/jats:sub>\n            s) for PBP1a from\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. baumannii<\/jats:named-content>\n            and PBP3 from\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Acinetobacter<\/jats:named-content>\n            sp. ranged from 1 to 5 \u03bcM for a series of \u03b2-lactams. In contrast, PBP3 demonstrated a narrower range of IC\n            <jats:sub>50<\/jats:sub>\n            s against \u03b2-lactamase inhibitors than PBP1a (ranges, 4 to 5 versus 8 to 144 \u03bcM, respectively). A molecular model with ampicillin and sulbactam positioned in the active site of PBP3 reveals that both compounds interact similarly with residues Thr526, Thr528, and Ser390. Accepting that many interactions with cell wall targets are possible with the ampicillin-sulbactam combination, the low IC\n            <jats:sub>50<\/jats:sub>\n            s of ampicillin and sulbactam for PBP3 may contribute to understanding why this combination is effective against\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. baumannii<\/jats:named-content>\n            . Unraveling the contribution of PBPs to \u03b2-lactam susceptibility and resistance brings us one step closer to identifying which PBPs are the best targets for novel \u03b2-lactams.\n          <\/jats:p>","DOI":"10.1128\/aac.01027-12","type":"journal-article","created":{"date-parts":[[2012,8,21]],"date-time":"2012-08-21T04:22:25Z","timestamp":1345522945000},"page":"5687-5692","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["Early Insights into the Interactions of Different \u03b2-Lactam Antibiotics and \u03b2-Lactamase Inhibitors against Soluble Forms of Acinetobacter baumannii PBP1a and Acinetobacter sp. 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