{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T14:18:03Z","timestamp":1785161883888,"version":"3.55.0"},"reference-count":78,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Canadian Government | Canadian Institutes of Health Research","award":["PJ4-175369,PJT-178191"],"award-info":[{"award-number":["PJ4-175369,PJT-178191"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2024,12,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Acinetobacter baumannii<\/jats:italic>\n              is globally recognized as a multi-drug-resistant pathogen of critical concern due to its capacity for horizontal gene transfer and resistance to antibiotics. Phylogenetically diverse\n              <jats:italic>Acinetobacter<\/jats:italic>\n              species mediate human infection, including many considered as important emerging pathogens. While globally recognized as a pathogen of concern, pathogenesis mechanisms are poorly understood. P-type type IV secretion systems (T4SSs) represent important drivers of pathogen evolution, responsible for horizontal gene transfer and secretion of proteins that mediate host-pathogen interactions, contributing to pathogen survival, antibiotic resistance, virulence, and biofilm formation. Genes encoding a P-type T4SS were previously identified on plasmids harboring the carbapenemase gene\n              <jats:italic>\n                bla\n                <jats:sub>NDM-1<\/jats:sub>\n              <\/jats:italic>\n              in several clinically problematic\n              <jats:italic>Acinetobacter<\/jats:italic>\n              ; however, their prevalence among the genus, geographical distribution, the conservation of T4SS proteins, and full capacity for resistance genes remain unclear. Using systematic analyses, we show that these plasmids belong to a group of 53 P-type T4SS-encoding plasmids in 20 established\n              <jats:italic>Acinetobacter<\/jats:italic>\n              species, the majority of clinical relevance, including diverse\n              <jats:italic>A. baumannii<\/jats:italic>\n              sequence types and one strain of\n              <jats:italic>Providencia rettgeri<\/jats:italic>\n              . The strains were globally distributed in 14 countries spanning five continents, and the conjugative operon\u2019s T4SS proteins were highly conserved in most plasmids. A high proportion of plasmids harbored resistance genes, with 17 different genes spanning seven drug classes. Collectively, this demonstrates that P-type T4SS-encoding plasmids are more widespread among the\n              <jats:italic>Acinetobacter<\/jats:italic>\n              genus than previously anticipated, including strains of both clinical and environmental importance. This research provides insight into the spread of resistance genes among\n              <jats:italic>Acinetobacter<\/jats:italic>\n              and highlights a group of plasmids of importance for future surveillance.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.01038-24","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T13:00:11Z","timestamp":1730725211000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Analysis of\n            <i>Acinetobacter<\/i>\n            P-type type IV secretion system-encoding plasmid diversity uncovers extensive secretion system conservation and diverse antibiotic resistance determinants"],"prefix":"10.1128","volume":"68","author":[{"given":"Mosopefoluwa T.","family":"Oke","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology, University of Ottawa","place":["Ottawa, Canada"]},{"name":"Centre for Infection, Immunity and Inflammation, University of Ottawa","place":["Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kailey","family":"Martz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology, University of Ottawa","place":["Ottawa, Canada"]},{"name":"Centre for Infection, Immunity and Inflammation, University of Ottawa","place":["Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u0103d\u0103lina","family":"Moc\u0103ni\u021b\u0103","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology, University of Ottawa","place":["Ottawa, Canada"]},{"name":"Centre for Infection, Immunity and Inflammation, University of Ottawa","place":["Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Knezevic","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology, University of Ottawa","place":["Ottawa, Canada"]},{"name":"Centre for Infection, Immunity and Inflammation, University of Ottawa","place":["Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4340-7778","authenticated-orcid":true,"given":"Vanessa M.","family":"D'Costa","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology, University of Ottawa","place":["Ottawa, Canada"]},{"name":"Centre for Infection, Immunity and Inflammation, University of Ottawa","place":["Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1789"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2015.1025055"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.4161\/viru.19700"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/MCC.0000000000000526"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-021-07024-4"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.12998\/wjcc.v2.i12.787"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S244044"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01089-20"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2009.01630.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00892-22"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.12691\/ajidm-8-4-3"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-818619-0.00029-0"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jambio\/lxad145"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1515\/reveh-2017-0034"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(23)00246-X"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03824-14"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11030759"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01289-22"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.001054"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00593"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.000043"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00049"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2019.12.010"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.24875\/BMHIM.21000041"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.2174\/1871526515666150826114745"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00926"},{"key":"e_1_3_3_28_2","first-page":"1","article-title":"Common bacterial causes of septicaemia in ani-mals and birds detected in heart blood samples of refer-red cases of mortality in Northern India","volume":"7","author":"Singh B","year":"2023","unstructured":"Singh B, Agri H, Karthikeyan R, Jayakumar V. 2023. Common bacterial causes of septicaemia in ani-mals and birds detected in heart blood samples of refer-red cases of mortality in Northern India. J Clin Med Img 7:1\u201314.","journal-title":"J Clin Med Img"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2020.108702"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/are.14584"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/1995494"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.31080\/ASMI.2022.05.1139"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02502-19"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/ecosalplus.ESP-0020-2015"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.3390\/genes11111239"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008582"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-16-1680"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00638-16"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv191"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa182"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1227063"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01455-15"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05168-14"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03242-14"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.06199-11"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/jacamr\/dlab191"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt066"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02102-22"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010034"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2021.145707"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-302"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039107"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.588410"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.01283"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.2289704"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2013.12.019"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2024.05.015"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0503-9"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz935"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa345"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.14408"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa390"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555819286"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02151"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evx115"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.12555"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-017-8392-4"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.4314\/ahs.v19i3.13"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1039\/C9MD00076C"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.9.4382-4390.2005"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2009.00168.x"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2024.2320929"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1177\/117693430600200031"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.8.754"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae268"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks261"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2021.106404"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.01038-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.01038-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T14:02:04Z","timestamp":1733407324000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aac.01038-24"}},"subtitle":[],"editor":[{"given":"Alessandra","family":"Carattoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,12,5]]},"references-count":78,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024,12,5]]}},"alternative-id":["10.1128\/aac.01038-24"],"URL":"https:\/\/doi.org\/10.1128\/aac.01038-24","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,5]]},"assertion":[{"value":"2024-07-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01038-24"}}