{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:22:07Z","timestamp":1785406927331,"version":"3.56.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,3,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Roseomonas mucosa<\/jats:italic>\n                      is an opportunistic bacterium found in clinical and environmental samples that primarily affects immunocompromised patients. Treatment is challenging owing to the lack of standardized susceptibility testing methods, breakpoints, and variable antimicrobial resistance profiles published. This study evaluated different antimicrobial susceptibility testing approaches and searched for new insights into resistance mechanisms, especially against third-generation cephalosporins. Antimicrobial susceptibility profiles of a panel of 17\n                      <jats:italic toggle=\"yes\">R<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">mucosa<\/jats:italic>\n                      strains were analyzed using disk diffusion method (DDM), broth microdilution (BMD) method, and MIC gradient strips. Discrepancies between susceptibility methods were further explored using beta-lactamase inhibitors, whole-genome sequencing, and transcriptomic analyses. Antimicrobial susceptibility testing revealed high susceptibility to aminoglycosides, fluoroquinolones, and carbapenems, while resistance to many beta-lactams was detected. Significant discrepancies were observed between the DDM and BMD methods, particularly with respect to the use of third- and fourth-generation cephalosporins and aztreonam. Genomic analysis identified two putative class-A and one class-C beta-lactamases within all strains. Transcription of one class A beta-lactamase, controlled by an\n                      <jats:italic toggle=\"yes\">lysR<\/jats:italic>\n                      regulator, was significantly induced by cephalosporins and explained the phenotype observed. This study provides the antimicrobial susceptibility profiles against a large panel of antibiotics from 17\n                      <jats:italic toggle=\"yes\">R<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">mucosa<\/jats:italic>\n                      strains. It also explained the deep discrepancies between phenotypic approaches regarding cephalosporins.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01041-25","type":"journal-article","created":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T14:38:53Z","timestamp":1769006333000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Phenotypic and genomic characterization of\n                    <i>Roseomonas mucosa<\/i>\n                    , an opportunistic pathogen with discrepancies among antimicrobial susceptibility testing methods"],"prefix":"10.1128","volume":"70","author":[{"given":"Camille","family":"Cotet","sequence":"first","affiliation":[{"name":"Department of Infectious Agents, Service of Bacteriology, Normandie Univ, UNICAEN, CHU de Caen Normandie","place":["Caen, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastien","family":"Galopin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051kpcy16","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 de Caen Normandie, Univ Rouen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311","place":["Caen, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Emeline","family":"Creach","sequence":"additional","affiliation":[{"name":"Department of Infectious Agents, Service of Bacteriology, Normandie Univ, UNICAEN, CHU de Caen Normandie","place":["Caen, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwan","family":"Tenouri","sequence":"additional","affiliation":[{"name":"Department of Infectious Agents, Service of Bacteriology, Normandie Univ, UNICAEN, CHU de Caen Normandie","place":["Caen, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Le Pont","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051kpcy16","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 de Caen Normandie, Univ Rouen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311","place":["Caen, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Laquay","sequence":"additional","affiliation":[{"name":"Normandie Univ, UNICAEN, CHU de Caen Normandie, Chirurgical Reanimation and Intensive Care Unit","place":["Caen, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8588-2732","authenticated-orcid":true,"given":"Jean-Christophe","family":"Giard","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051kpcy16","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 de Caen Normandie, Univ Rouen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311","place":["Caen, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7993-4305","authenticated-orcid":true,"given":"Simon","family":"Le Hello","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051kpcy16","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Infectious Agents, Service of Bacteriology, Universit\u00e9 de Caen Normandie, Univ Rouen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311, CHU Caen Normandie","place":["Caen, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8672-8286","authenticated-orcid":false,"given":"Fran\u00e7ois","family":"Gravey","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051kpcy16","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Infectious Agents, Service of Bacteriology, Universit\u00e9 de Caen Normandie, Univ Rouen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311, CHU Caen Normandie","place":["Caen, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.12.3275-3283.1993"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1309\/731V-VGVC-KK35-1Y4J"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2016.05.024"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-3774-0"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.2169\/internalmedicine.2998-23"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00701-020-04449-9"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/acmi.0.000213"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-017-2538-4"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1080\/1120009X.2020.1785742"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2011.11.013"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24277-4_9"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw354"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.102"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt086"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.055459-0"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks261"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz935"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-0997-x"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1086\/416571"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae268"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2020.03.007"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2016.36.4.367"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-023-08875-9"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa345"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2024.105610"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.01041-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.01041-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T14:01:39Z","timestamp":1772632899000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aac.01041-25"}},"subtitle":[],"editor":[{"given":"Alessandra","family":"Carattoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,3,4]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3,4]]}},"alternative-id":["10.1128\/aac.01041-25"],"URL":"https:\/\/doi.org\/10.1128\/aac.01041-25","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,4]]},"assertion":[{"value":"2025-07-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01041-25"}}