{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T22:33:58Z","timestamp":1783636438156,"version":"3.55.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T00:00:00Z","timestamp":1767744000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T00:00:00Z","timestamp":1767744000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Basilea Pharmaceutica International, Ltd., Allschwil"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,1,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Despite the use of recommended treatments against\n                      <jats:italic toggle=\"yes\">Enterococcus faecalis<\/jats:italic>\n                      infective endocarditis, mortality rates remain at 30%. Penicillin-resistant, ampicillin-susceptible\n                      <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                      (PRASEF) has been associated with worsened clinical outcomes; however, a more common phenotype, borderline-PRASEF (penicillin minimum inhibitory concentration [MIC] 4\u20138 \u00b5g\/mL), decreases the activity of ampicillin-ceftriaxone\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      . Ceftobiprole presents a promising alternative against borderline-PRASEF and also has shown activity against vancomycin-resistant\n                      <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                      (VREfs). The ceftobiprole MIC distribution was determined via broth microdilution for 78\n                      <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                      clinical blood isolates, including 71 borderline-PRASEF. Ceftobiprole activity alone was compared to ampicillin-ceftriaxone via\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      24-hour time-kill assays against 30\n                      <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                      isolates, 24 of which were borderline-PRASEF and 15 were VREfs. Ceftobiprole and ceftriaxone were tested at physiologic concentrations (\n                      <jats:italic toggle=\"yes\">fCpss<\/jats:italic>\n                      13.9 \u00b5g\/mL and 17.2 mcg\/mL, respectively) and ampicillin at subinhibitory concentrations (0.25 \u00d7 MIC and 0.5 \u00d7 MIC). Isolates where ceftobiprole alone had limited activity were tested against ceftobiprole-ampicillin. Ceftobiprole maintained activity against borderline-PRASEF compared to ampicillin-ceftriaxone. Ceftobiprole also demonstrated more activity against VREfs isolates and isolates with an elevated ceftobiprole MIC \u226516 \u00b5g\/mL, compared to ampicillin-ceftriaxone. Ceftobiprole did not achieve \u22652 log\n                      <jats:sub>10<\/jats:sub>\n                      CFU\/mL kill in six isolates but was able to achieve this once combined with ampicillin. When tested at ampicillin inhibitory concentrations, ceftobiprole-ampicillin activity trended towards antagonism in two isolates. Overall, ceftobiprole alone had greater activity compared to ampicillin-ceftriaxone, and there may be a potential role of ceftobiprole combination therapy in certain isolates.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01050-25","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T13:00:30Z","timestamp":1763989230000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Ceftobiprole alone versus ampicillin-ceftriaxone against borderline-penicillin-resistant, ampicillin-susceptible, and vancomycin-resistant\n                    <i>Enterococcus faecalis<\/i>\n                    isolates"],"prefix":"10.1128","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1069-579X","authenticated-orcid":false,"given":"Olivia Gladys","family":"Funk","sequence":"first","affiliation":[{"name":"Division of Pharmacy Practice, Arnold and Marie Schwartz College of Pharmacy and Health Sciences, Long Island University","place":["Brooklyn, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyi","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Pharmacy Practice, Arnold and Marie Schwartz College of Pharmacy and Health Sciences, Long Island University","place":["Brooklyn, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ifra","family":"Khan","sequence":"additional","affiliation":[{"name":"Division of Pharmacy Practice, Arnold and Marie Schwartz College of Pharmacy and Health Sciences, Long Island University","place":["Brooklyn, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3207-7320","authenticated-orcid":true,"given":"Jaclyn A.","family":"Cusumano","sequence":"additional","affiliation":[{"name":"Division of Pharmacy Practice, Arnold and Marie Schwartz College of Pharmacy and Health Sciences, Long Island University","place":["Brooklyn, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000000296"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2796.2002.01061.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciad431"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehad193"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00392-021-01971-3"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofab102"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0192387"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.3.639"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit052"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehz620"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12872-021-01853-6"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy064"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00291-19"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.816657"},{"key":"e_1_3_3_16_2","volume-title":"M100 Performance standards for antimicrobial susceptibility testing","author":"Clinical and Laboratory Standards Institute","year":"2023","unstructured":"Clinical and Laboratory Standards Institute. 2023. M100 Performance standards for antimicrobial susceptibility testing. 33rd ed","edition":"33"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz594"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofad500.257"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00621-22"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00871-22"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00131-07"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12050879"},{"key":"e_1_3_3_23_2","first-page":"292","article-title":"One stage extraction and reimplantation of ICD\/PM in patients with CIED related endocarditis and spondiloscitis due to E. faecalis treated with double beta-lactam combination: ampicillin plus ceftobiprole","volume":"47","author":"Narducci ML","year":"2024","unstructured":"Narducci ML, Pecori D, Imazio M, Rebellato L, Geminiani M, Bontempo G, Martini L, Giuliano S, Tascini C. 2024. One stage extraction and reimplantation of ICD\/PM in patients with CIED related endocarditis and spondiloscitis due to E. faecalis treated with double beta-lactam combination: ampicillin plus ceftobiprole. 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