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Dosing regimens can vary in dose level, dosing interval, and treatment duration. The current studies examined the relationships between antimicrobial dosing regimens and resistance development by use of an in vivo model. A murine model of systemic<jats:italic>Candida albicans<\/jats:italic>infection was used to examine resistance emergence during exposure to the triazole antifungal fluconazole. Data from this experimental model demonstrated that the more frequently administered dosing prevented selection of the isogenic resistant cell populations. Conversely, dosing regimens producing prolonged sub-MIC effects appeared to contribute to the outgrowth of isogenic resistant strains. The association between dosing and resistance emergence observed in the current investigation is disparate from that described for antimicrobial compounds with cidal killing characteristics. The inhibitory or static antimicrobial activity of the triazole compounds may explain these differences.<\/jats:p>","DOI":"10.1128\/aac.01053-05","type":"journal-article","created":{"date-parts":[[2006,6,26]],"date-time":"2006-06-26T18:26:28Z","timestamp":1151346388000},"page":"2374-2383","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":69,"title":["Impact of Antimicrobial Dosing Regimen on Evolution of Drug Resistance In Vivo: Fluconazole and<i>Candida albicans<\/i>"],"prefix":"10.1128","volume":"50","author":[{"given":"D.","family":"Andes","sequence":"first","affiliation":[{"name":"School of Pharmacy, SUNY, Buffalo, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Forrest","sequence":"additional","affiliation":[{"name":"School of Pharmacy, SUNY, Buffalo, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Lepak","sequence":"additional","affiliation":[{"name":"Department of Medicine and Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Nett","sequence":"additional","affiliation":[{"name":"Department of Medicine and Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Marchillo","sequence":"additional","affiliation":[{"name":"Department of Medicine and Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Lincoln","sequence":"additional","affiliation":[{"name":"Department of Medicine and Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.9.2116"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.4.1179-1186.2003"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2004.05.003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.6.1578-1584.2000"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.1.108-114.2001"},{"key":"e_1_3_2_7_2","first-page":"13285","volume":"101","year":"2005","unstructured":"Bergstrom, C. 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