{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T07:03:30Z","timestamp":1792134210036,"version":"4.4.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Clinical and Laboratory Standards Institute (CLSI) conditions for testing the susceptibilities of pathogenic<jats:named-content content-type=\"genus-species\">Sporothrix<\/jats:named-content>species to antifungal agents are based on a collaborative study that evaluated five clinically relevant isolates of<jats:named-content content-type=\"genus-species\">Sporothrix<\/jats:named-content><jats:named-content content-type=\"genus-species\">schenckii sensu lato<\/jats:named-content>and some antifungal agents. With the advent of molecular identification, there are two basic needs: to confirm the suitability of these testing conditions for all agents and<jats:named-content content-type=\"genus-species\">Sporothrix<\/jats:named-content>species and to establish species-specific epidemiologic cutoff values (ECVs) or breakpoints (BPs) for the species. We collected available CLSI MICs\/minimal effective concentrations (MECs) of amphotericin B, five triazoles, terbinafine, flucytosine, and caspofungin for 301<jats:named-content content-type=\"genus-species\">Sporothrix schenckii sensu stricto<\/jats:named-content>, 486<jats:named-content content-type=\"genus-species\">S. brasiliensis<\/jats:named-content>, 75<jats:named-content content-type=\"genus-species\">S. globosa<\/jats:named-content>, and 13<jats:named-content content-type=\"genus-species\">S. mexicana<\/jats:named-content>molecularly identified isolates. Data were obtained in 17 independent laboratories (Australia, Europe, India, South Africa, and South and North America) using conidial inoculum suspensions and 48 to 72 h of incubation at 35\u00b0C. Sufficient and suitable data (modal MICs within 2-fold concentrations) allowed the proposal of the following ECVs for<jats:named-content content-type=\"genus-species\">S. schenckii<\/jats:named-content>and<jats:named-content content-type=\"genus-species\">S. brasiliensis<\/jats:named-content>, respectively: amphotericin B, 4 and 4 \u03bcg\/ml; itraconazole, 2 and 2 \u03bcg\/ml; posaconazole, 2 and 2 \u03bcg\/ml; and voriconazole, 64 and 32 \u03bcg\/ml. Ketoconazole and terbinafine ECVs for<jats:named-content content-type=\"genus-species\">S. brasiliensis<\/jats:named-content>were 2 and 0.12 \u03bcg\/ml, respectively. Insufficient or unsuitable data precluded the calculation of ketoconazole and terbinafine (or any other antifungal agent) ECVs for<jats:named-content content-type=\"genus-species\">S. schenckii<\/jats:named-content>, as well as ECVs for<jats:named-content content-type=\"genus-species\">S. globosa<\/jats:named-content>and<jats:named-content content-type=\"genus-species\">S. mexicana<\/jats:named-content>. These ECVs could aid the clinician in identifying potentially resistant isolates (non-wild type) less likely to respond to therapy.<\/jats:p>","DOI":"10.1128\/aac.01057-17","type":"journal-article","created":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T00:20:23Z","timestamp":1500942023000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":104,"title":["Multicenter, International Study of MIC\/MEC Distributions for Definition of Epidemiological Cutoff Values for Sporothrix Species Identified by Molecular Methods"],"prefix":"10.1128","volume":"61","author":[{"given":"A.","family":"Espinel-Ingroff","sequence":"first","affiliation":[{"name":"VCU Medical Center, Richmond, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. P. 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