{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T11:34:49Z","timestamp":1789385689218,"version":"build-2803163510"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Two clinical isolates of\n            <jats:italic>Escherichia coli<\/jats:italic>\n            , CF1179 and CF1295, were isolated from a patient hospitalized in the hematology unit of the University Hospital of Clermont-Ferrand, Clermont-Ferrand, France. They were resistant to penicillin-clavulanate combinations and to ceftazidime. The double-disk synergy test was positive only for isolate CF1179. Molecular comparison of the isolates showed that they were clonally related.\n            <jats:italic>E. coli<\/jats:italic>\n            recombinant strains exhibiting the resistance phenotype of the clinical strains were obtained by cloning. The clones corresponding to strains CF1179 and CF1295 produced TEM-type beta-lactamases with pI values of 5.7 and 5.3, respectively. Sequencing analysis revealed two novel\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM<\/jats:sub>\n            genes encoding closely related complex mutant TEM enzymes, designated TEM-151 (pI 5.3) and TEM-152 (pI 5.7). These two genes also harbored a new promoter region which presented a 9-bp deletion. The two novel \u03b2-lactamases differed from the parental enzyme, TEM-1, by the substitution Arg164His, previously observed in extended-spectrum beta-lactamases (ESBLs), and by the substitutions Met69Val and Asn276Asp, previously observed in the inhibitor-resistant penicillinase TEM-36\/IRT-7. They differed by two amino acid substitutions: TEM-152 harbored a Glu240Lys ESBL-type substitution and TEM-151 had an Ala284Gly substitution. Functional analysis of TEM-151 and TEM-152 showed that both enzymes had hydrolytic activity against ceftazidime (\n            <jats:italic>k<\/jats:italic>\n            <jats:sub>cat<\/jats:sub>\n            , 5 and 16 s\n            <jats:sup>\u22121<\/jats:sup>\n            , respectively). TEM-152 was more resistant than TEM-151 to the inhibitor clavulanic acid (50% inhibitory concentrations, 1 versus 0.17 \u03bcM). These results confirm the evolution of TEM-type enzymes toward complex enzymes harboring the two kinds of substitutions which confer an extended spectrum of action against beta-lactam antibiotics and resistance to inhibitors.\n          <\/jats:p>","DOI":"10.1128\/aac.01058-06","type":"journal-article","created":{"date-parts":[[2007,1,13]],"date-time":"2007-01-13T01:49:47Z","timestamp":1168652987000},"page":"1304-1309","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Evolution of TEM-Type Enzymes: Biochemical and Genetic Characterization of Two New Complex Mutant TEM Enzymes, TEM-151 and TEM-152, from a Single Patient"],"prefix":"10.1128","volume":"51","author":[{"given":"Fre\u0301de\u0301ric","family":"Robin","sequence":"first","affiliation":[{"name":"CHU Clermont-Ferrand, Centre de Biologie, Laboratoire de Bacte\u0301riologie Clinique, Clermont-Ferrand F-63003, France"},{"name":"Universite\u0301 Clermont 1, UFR Me\u0301decine, Laboratoire de Bacte\u0301riologie, EA3844, Clermont-Ferrand F-63001, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Delmas","sequence":"additional","affiliation":[{"name":"CHU Clermont-Ferrand, Centre de Biologie, Laboratoire de Bacte\u0301riologie Clinique, Clermont-Ferrand F-63003, France"},{"name":"Universite\u0301 Clermont 1, UFR Me\u0301decine, Laboratoire de Bacte\u0301riologie, EA3844, Clermont-Ferrand F-63001, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ce\u0301dric","family":"Schweitzer","sequence":"additional","affiliation":[{"name":"CHU Clermont-Ferrand, Centre de Biologie, Laboratoire de Bacte\u0301riologie Clinique, Clermont-Ferrand F-63003, France"},{"name":"Universite\u0301 Clermont 1, UFR Me\u0301decine, Laboratoire de Bacte\u0301riologie, EA3844, Clermont-Ferrand F-63001, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Tournilhac","sequence":"additional","affiliation":[{"name":"CHU Clermont-Ferrand, Ho\u0302tel-Dieu, Service d'He\u0301matologie Clinique, Clermont-Ferrand F-63003, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Lesens","sequence":"additional","affiliation":[{"name":"CHU Clermont-Ferrand, Ho\u0302tel-Dieu, Service de Maladies Infectieuses et Tropicales, Clermont-Ferrand F-63003, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Chanal","sequence":"additional","affiliation":[{"name":"CHU Clermont-Ferrand, Centre de Biologie, Laboratoire de Bacte\u0301riologie Clinique, Clermont-Ferrand F-63003, France"},{"name":"Universite\u0301 Clermont 1, UFR Me\u0301decine, Laboratoire de Bacte\u0301riologie, EA3844, Clermont-Ferrand F-63001, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Bonnet","sequence":"additional","affiliation":[{"name":"CHU Clermont-Ferrand, Centre de Biologie, Laboratoire de Bacte\u0301riologie Clinique, Clermont-Ferrand F-63003, France"},{"name":"Universite\u0301 Clermont 1, UFR Me\u0301decine, Laboratoire de Bacte\u0301riologie, EA3844, Clermont-Ferrand F-63001, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","first-page":"75","volume":"120","year":"1994","unstructured":"Belaaouaj, A., C. Lapoumeroulie, M. M. Canica, G. Vedel, P. Nevot, R. Krishnamoorthy, and G. Paul. 1994. Nucleotide sequences of the genes coding for the TEM-like \u03b2-lactamases IRT-1 and IRT-2 (formerly called TRI-1 and TRI-2). FEMS Microbiol. Lett.120:75-80.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.11.3061-3068.2000"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.1.260"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/43.4.447"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.02.010"},{"key":"e_1_3_2_8_2","unstructured":"Clinical Laboratory Standards Institute (CLSI). 2005. Performance standards for antimicrobial susceptibility testing; 15th informational supplement (M100-S15). CLSI Wayne PA."},{"key":"e_1_3_2_9_2","unstructured":"Comit\u00e9 de l'Antibiogramme de la Soci\u00e9t\u00e9 Fran\u00e7aise de Microbiologie. 2006. 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Delmas, M. Archambaud, C. Schweitzer, C. Chanal, and R. Bonnet. 2006. CMT-type \u03b2-lactamase TEM-125, an emerging problem for extended-spectrum \u03b2-lactamase detection. Antimicrob. Agents Chemother.50:2043-2048.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.11.4443-4447.2005"},{"key":"e_1_3_2_26_2","unstructured":"Sambrook J. E. F. Tritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. vol. 1 p. 1659. Cold Spring Harbor Laboratory Press Cold Spring Harbor NY."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.6.1322"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/20.3.323"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/10.4.850"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.8.3737"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01058-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01058-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T19:21:06Z","timestamp":1645471266000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01058-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4]]},"references-count":30,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,4]]}},"alternative-id":["10.1128\/AAC.01058-06"],"URL":"https:\/\/doi.org\/10.1128\/aac.01058-06","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4]]},"assertion":[{"value":"2006-08-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-01-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}