{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T15:19:33Z","timestamp":1773242373967,"version":"3.50.1"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:00:00Z","timestamp":1214870400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Metallo-\u03b2-lactamases (MBLs) are zinc-dependent bacterial enzymes characterized by an efficient hydrolysis of carbapenems and a lack of sensitivity to commercially available \u03b2-lactamase inactivators. Apart from the acquired subclass B1 enzymes, which exhibit increasing clinical importance and whose evolutionary origin remains unclear, most MBLs are encoded by resident genes found in the genomes of organisms belonging to at least three distinct phyla. Using genome database mining, we identified an open reading frame (ORF) (ECA2849) encoding an MBL-like protein in the sequenced genome of\n            <jats:italic>Erwinia carotovora<\/jats:italic>\n            , an important plant pathogen. Although no detectable \u03b2-lactamase activity could be found in\n            <jats:italic>E. carotovora<\/jats:italic>\n            , a recombinant\n            <jats:italic>Escherichia coli<\/jats:italic>\n            strain in which the ECA2849 ORF was cloned showed decreased susceptibility to several \u03b2-lactams, while carbapenem MICs were surprisingly poorly affected. The enzyme, named CAR-1, was purified by means of ion-exchange chromatography steps, and its characterization revealed unique structural and functional features. This new MBL was able to efficiently hydrolyze cephalothin, cefuroxime, and cefotaxime and, to a lesser extent, penicillins and the other cephalosporins but only poorly hydrolyzed meropenem, while imipenem was not recognized. CAR-1 is the first example of a functional naturally occurring MBL in the family\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            (order\n            <jats:italic>Enterobacteriales<\/jats:italic>\n            ) and highlights the extraordinary structural and functional diversity exhibited by MBLs.\n          <\/jats:p>","DOI":"10.1128\/aac.01062-07","type":"journal-article","created":{"date-parts":[[2008,4,29]],"date-time":"2008-04-29T00:53:45Z","timestamp":1209430425000},"page":"2473-2479","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Functional Diversity among Metallo-\u03b2-Lactamases: Characterization of the CAR-1 Enzyme of\n            <i>Erwinia carotovora<\/i>"],"prefix":"10.1128","volume":"52","author":[{"given":"Magdalena","family":"Stoczko","sequence":"first","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Laboratorio di Fisiologia e Biotecnologia dei Microrganismi, Universita\u0300 di Siena, I-53100, Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Marie","family":"Fre\u0300re","sequence":"additional","affiliation":[{"name":"Centre d'Inge\u0301nierie des Prote\u0301ines and Laboratoire d'Enzymologie, Universite\u0301 de Lie\u0300ge, B-4000 Lie\u0300ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gian Maria","family":"Rossolini","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Laboratorio di Fisiologia e Biotecnologia dei Microrganismi, Universita\u0300 di Siena, I-53100, Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Denis","family":"Docquier","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Laboratorio di Fisiologia e Biotecnologia dei Microrganismi, Universita\u0300 di Siena, I-53100, Siena, Italy"},{"name":"Centre d'Inge\u0301nierie des Prote\u0301ines and Laboratoire d'Enzymologie, Universite\u0301 de Lie\u0300ge, B-4000 Lie\u0300ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1098\/rstb.1980.0049","volume":"289","year":"1980","unstructured":"Ambler, R. 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