{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:53:16Z","timestamp":1792068796785,"version":"4.4.0"},"reference-count":10,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Susceptibility to several \u03b2-lactams and \u03b2-lactamase production was investigated in a collection of 20 strains of\n                    <jats:italic>Pseudomonas otitidis<\/jats:italic>\n                    , a new\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    species that has been recently recognized in association with otic infections in humans. All strains appeared to be susceptible to piperacillin, cefotaxime, ceftazidime, and aztreonam, while resistance or decreased susceptibility to carbapenems was occasionally observed. All strains were found to express metallo-\u03b2-lactamase (MBL) activity and to carry a new subclass B3 MBL gene, named\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>POM<\/jats:sub>\n                    , that appeared to be highly conserved in this species.\n                    <jats:italic>P. otitidis<\/jats:italic>\n                    , therefore, is the first example of a pathogenic\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    species endowed with a resident MBL. The POM-1 protein from\n                    <jats:italic>P. otitidis<\/jats:italic>\n                    type strain MCC10330 exhibits the closest similarity (60 to 64%) to the L1 MBL of\n                    <jats:italic>Stenotrophomonas maltophilia<\/jats:italic>\n                    . Expression in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    and\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    revealed that, similar to L1 and other subclass B3 MBLs, POM-1 confers decreased susceptibility or resistance to carbapenems, penicillins, and cephalosporins but not to aztreonam. Expression of the POM MBL in\n                    <jats:italic>P. otitidis<\/jats:italic>\n                    is apparently constitutive and, in most strains, does not confer a carbapenem-resistant phenotype. However, a strong inoculum size effect was observed for carbapenem MICs, and carbapenem-resistant mutants could be readily selected upon exposure to imipenem, suggesting that carbapenem-based regimens should be considered with caution for\n                    <jats:italic>P. otitidis<\/jats:italic>\n                    infections.\n                  <\/jats:p>","DOI":"10.1128\/aac.01062-10","type":"journal-article","created":{"date-parts":[[2010,11,9]],"date-time":"2010-11-09T02:14:39Z","timestamp":1289268879000},"page":"118-123","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":48,"title":["Metallo-\u03b2-Lactamase Production by\n                    <i>Pseudomonas otitidis<\/i>\n                    : a Species-Related Trait"],"prefix":"10.1128","volume":"55","author":[{"given":"Maria Cristina","family":"Thaller","sequence":"first","affiliation":[{"name":"Dipartimento di Biologia, Universita\u0300 di Roma \u201cTor Vergata,\u201d I-00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa","family":"Borgianni","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Universita\u0300 di Siena, I-53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Di Lallo","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia, Universita\u0300 di Roma \u201cTor Vergata,\u201d I-00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunsop","family":"Chong","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyungwon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Dajcs","sequence":"additional","affiliation":[{"name":"Alcon Research Ltd., Fort Worth, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Stroman","sequence":"additional","affiliation":[{"name":"Alcon Research Ltd., Fort Worth, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gian Maria","family":"Rossolini","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Universita\u0300 di Siena, I-53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.24.14073"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63753-0"},{"key":"e_1_3_2_4_2","unstructured":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 2009"},{"key":"e_1_3_2_5_2","unstructured":"Performance standards for antimicrobial susceptibility testing. 2010"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.6.1823-1830.2002"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.7.2347-2349.2004"},{"key":"e_1_3_2_8_2","first-page":"115","year":"1991","unstructured":"Lane, D. J. 1991. 16S\/23S rRNA sequencing, p. 115-175. In E. Stackebrandt and M. Goodfellow (ed.), Nucleic acid techniques in bacterial systematics. John Wiley & Sons, Inc., New York, NY.","journal-title":"Nucleic acid techniques in bacterial systematics"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.03116.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.3.837-844.2001"},{"key":"e_1_3_2_11_2","unstructured":"Molecular cloning: a laboratory manual 2001"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01062-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01062-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T16:54:49Z","timestamp":1645462489000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01062-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,1]]}},"alternative-id":["10.1128\/AAC.01062-10"],"URL":"https:\/\/doi.org\/10.1128\/aac.01062-10","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,1]]},"assertion":[{"value":"2010-08-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-10-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}