{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T09:36:19Z","timestamp":1791020179662,"version":"4.1.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2008,2,1]],"date-time":"2008-02-01T00:00:00Z","timestamp":1201824000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>erm<\/jats:italic>\n            (A) subclass\n            <jats:italic>erm<\/jats:italic>\n            (TR), a common macrolide resistance determinant in\n            <jats:italic>Streptococcus pyogenes<\/jats:italic>\n            but quite rare in\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            , was found in a clinical\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            isolate (AP200) from Italy. In this isolate,\n            <jats:italic>erm<\/jats:italic>\n            (TR) was found included in a genetic element approximately 56 kb in size that did not appear to be conjugative but could be transferred by transformation. An\n            <jats:italic>erm<\/jats:italic>\n            (TR)-containing DNA fragment of approximately 10 kb was sequenced and 12 open reading frames (ORFs) were identified. Upstream of\n            <jats:italic>erm<\/jats:italic>\n            (TR), a regulatory protein of the TetR family and the two components of an efflux pump of the ABC type were found. Downstream of\n            <jats:italic>erm<\/jats:italic>\n            (TR), there were ORFs homologous to a spectinomycin phosphotransferase, transposases, and a relaxase. Since the genomic sequence of\n            <jats:italic>S. pyogenes<\/jats:italic>\n            MGAS10750 carrying\n            <jats:italic>erm<\/jats:italic>\n            (TR) became available, comparison between the\n            <jats:italic>erm<\/jats:italic>\n            (TR)-containing genetic elements in AP200 and in MGAS10750 was performed. The region flanking\n            <jats:italic>erm<\/jats:italic>\n            (TR) in MGAS10750 showed identity with AP200 for 10 ORFs out of 12. PCR mapping using primers designed on the sequence of MGAS10750 confirmed that AP200 carries a genetic element similar to that of MGAS10750. In AP200 the genetic element was inserted inside an ORF homologous to spr0790 of\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            R6, coding for a type I restriction modification system. Homologies between the insertion sites in AP200 and MGAS10750 consisted of eight conserved nucleotides, of which three were duplicated, likely representing target site duplication. The structure of the\n            <jats:italic>erm<\/jats:italic>\n            (TR)-carrying genetic element shows characteristics of a transposon\/prophage remnant chimera. In AP200 this genetic element was designated Tn\n            <jats:italic>1806<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.01081-07","type":"journal-article","created":{"date-parts":[[2007,12,11]],"date-time":"2007-12-11T01:44:26Z","timestamp":1197337466000},"page":"619-625","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["New Genetic Element Carrying the Erythromycin Resistance Determinant\n            <i>erm<\/i>\n            (TR) in\n            <i>Streptococcus pneumoniae<\/i>"],"prefix":"10.1128","volume":"52","author":[{"given":"Romina","family":"Camilli","sequence":"first","affiliation":[{"name":"Department of Infectious, Parasitic and Immunemediated Diseases, Istituto Superiore di Sanita\u0300, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Del Grosso","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immunemediated Diseases, Istituto Superiore di Sanita\u0300, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Iannelli","sequence":"additional","affiliation":[{"name":"Laboratorio di Microbiologia Molecolare e Biotecnologia, Dipartimento di Biologia Molecolare, Universita\u0300 di Siena, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annalisa","family":"Pantosti","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immunemediated Diseases, Istituto Superiore di Sanita\u0300, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/379897"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000800"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510279103"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1838-1841.2000"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1574"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28417-0"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(03)00071-8"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1570"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.7.3031-3033.2005"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.3.774-778.2002"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.6.2037-2042.2004"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2005.02.010"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.7.3070-3072.2005"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkf806"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.764-768.2004"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/48.3.411"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.12.3750-3755.2002"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkf122"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02789105"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.5.3077-3080.2004"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1385\/MB:26:1:81"},{"key":"e_1_3_2_23_2","first-page":"525","volume":"21","year":"2007","unstructured":"Iannelli, F., and G. 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