{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T23:44:00Z","timestamp":1789861440230,"version":"4.0.1"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T00:00:00Z","timestamp":1204329600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Penicillin-binding proteins (PBPs) in representatives of two\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            clonal groups that are prevalent in Poland, Poland\n            <jats:sup>23F<\/jats:sup>\n            -16 and Poland\n            <jats:sup>6B<\/jats:sup>\n            -20, were investigated by PBP profile analysis, antibody reactivity pattern analysis, and DNA sequence analysis of the transpeptidase (TP) domain-encoding regions of the\n            <jats:italic>pbp2x<\/jats:italic>\n            ,\n            <jats:italic>pbp2b<\/jats:italic>\n            , and\n            <jats:italic>pbp1a<\/jats:italic>\n            genes. The isolates differed in their MICs of \u03b2-lactam antibiotics. The majority of the 6B isolates were intermediately susceptible to penicillin (penicillin MICs, 0.12 to 0.5 \u03bcg\/ml), whereas all 23F isolates were penicillin resistant (MICs, \u22652 \u03bcg\/ml). The 6B isolates investigated had the same sequence type (ST), determined by multilocus sequence typing, as the Poland\n            <jats:sup>6B<\/jats:sup>\n            -20 reference strain (ST315), but in the 23F group, isolates with three distinct single-locus variants (SLVs) in the\n            <jats:italic>ddl<\/jats:italic>\n            gene (ST173, ST272, and ST1506) were included. None of the isolates showed an identical PBP profile after labeling with Bocillin FL and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and only one pair of 6B isolates and one pair of 23F isolates (ST173 and ST272) each contained an identical combination of PBP 2x, PBP 2b, and PBP 1a TP domains. Some 23F isolates contained PBP 3 with an apparently higher electrophoretic mobility, and this feature also did not correlate with their STs. The data document a highly variable pool of PBP genes as a result of multiple gene transfer and recombination events within and between different clonal groups.\n          <\/jats:p>","DOI":"10.1128\/aac.01082-07","type":"journal-article","created":{"date-parts":[[2007,12,27]],"date-time":"2007-12-27T01:25:24Z","timestamp":1198718724000},"page":"1021-1027","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Highly Variable Penicillin Resistance Determinants PBP 2x, PBP 2b, and PBP 1a in Isolates of Two\n            <i>Streptococcus pneumoniae<\/i>\n            Clonal Groups, Poland\n            <sup>23F<\/sup>\n            -16 and Poland\n            <sup>6B<\/sup>\n            -20"],"prefix":"10.1128","volume":"52","author":[{"given":"Rados\u0142aw","family":"Izdebski","sequence":"first","affiliation":[{"name":"National Medicines Institute, Division of Clinical Microbiology and Infection Prevention, Che\u0142mska 30\/34, Warsaw 00-725, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Rutschmann","sequence":"additional","affiliation":[{"name":"University of Kaiserslautern, Department of Microbiology, Paul-Ehrlich Strasse 23, Kaiserslautern D-67663, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janusz","family":"Fiett","sequence":"additional","affiliation":[{"name":"National Medicines Institute, Division of Clinical Microbiology and Infection Prevention, Che\u0142mska 30\/34, Warsaw 00-725, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Sadowy","sequence":"additional","affiliation":[{"name":"National Medicines Institute, Division of Clinical Microbiology and Infection Prevention, Che\u0142mska 30\/34, Warsaw 00-725, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marek","family":"Gniadkowski","sequence":"additional","affiliation":[{"name":"National Medicines Institute, Division of Clinical Microbiology and Infection Prevention, Che\u0142mska 30\/34, Warsaw 00-725, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waleria","family":"Hryniewicz","sequence":"additional","affiliation":[{"name":"National Medicines Institute, Division of Clinical Microbiology and Infection Prevention, Che\u0142mska 30\/34, Warsaw 00-725, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regine","family":"Hakenbeck","sequence":"additional","affiliation":[{"name":"University of Kaiserslautern, Department of Microbiology, Paul-Ehrlich Strasse 23, Kaiserslautern D-67663, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.5.1252"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.9.2267"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1995.tb07433.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M511506200"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.7.2895-2902.2005"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2007.02.009"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb02155.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.10.030"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01723.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(94)90612-2"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.22.8842"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1989.tb00108.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.15.5858"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026082"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.45.100191.000345"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.66.4.702-738.2002"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1093\/jac\/dki118","volume":"55","year":"2005","unstructured":"Granger, D., G. Boily-Larouche, P. Turgeon, K. Weiss, and M. Roger. 2005. Genetic analysis of pbp2x in clinical Streptococcus pneumoniae isolates in Quebec, Canada. J. Antimicrob. Chemother.55:832-839.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki401"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.4.829"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.4.2477-2486.2001"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/164.2.307"},{"key":"e_1_3_2_23_2","first-page":"755","volume":"133","year":"1987","unstructured":"Hakenbeck, R., S. Tornette, and N. F. Adkinson. 1987. Interaction of non-lytic \u03b2-lactams with penicillin-binding proteins in Streptococcus pneumoniae. J. Gen. Microbiol.133:755-760.","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1093\/infdis\/159.1.16","volume":"159","year":"1989","unstructured":"Jabes, D., S. Nachman, and A. Tomasz. 1989. Penicillin-binding protein families: evidence for the clonal nature of penicillin resistance in clinical isolates of pneumococci. J. Infect. Dis.159:16-25.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.5.936"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-3002(60)90205-5"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb00821.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05475.x"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.7.2565-2571.2001"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.27.19175"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.23.13403"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/164.2.302"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01422.x"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/15.1.112"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.6.1739-1745.1999"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.5.1848-1855.2004"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313492200"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.1996.2.177"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-43-5-377"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01072-06"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb01089.x"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.6.1329"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.11.4622-4627.2005"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2007.03.004"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.5.1124"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01082-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01082-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T19:32:18Z","timestamp":1645471938000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01082-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,3]]},"references-count":45,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2008,3]]}},"alternative-id":["10.1128\/AAC.01082-07"],"URL":"https:\/\/doi.org\/10.1128\/aac.01082-07","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,3]]},"assertion":[{"value":"2007-08-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-12-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}