{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:38:16Z","timestamp":1773283096281,"version":"3.50.1"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 103-2320-B-002-056-MY3"],"award-info":[{"award-number":["MOST 103-2320-B-002-056-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We determined the resistance determinants in 274 erythromycin-resistant methicillin-susceptible\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Staphylococcus aureus<\/jats:named-content>\n            (MSSA) isolates during a 13-year period, 2000 to 2012. The resistance phenotypes, inducible macrolide-lincosamide-streptogramin (iMLS), constitutive MLS (cMLS), and macrolide-streptogramin (MS) resistance phenotypes, were examined by a double-disk diffusion D test. The\n            <jats:italic>ermB<\/jats:italic>\n            gene was more frequent (35%; 97\/274) than\n            <jats:italic>ermC<\/jats:italic>\n            (27%; 75\/274) or\n            <jats:italic>ermA<\/jats:italic>\n            (21%; 58\/274). All 97\n            <jats:italic>ermB<\/jats:italic>\n            -positive isolates harbored Tn\n            <jats:italic>551<\/jats:italic>\n            and IS\n            <jats:italic>1216V<\/jats:italic>\n            . The majority (89\/97) of\n            <jats:italic>ermB<\/jats:italic>\n            -positive isolates displayed the cMLS phenotype and carried mobile element structure (MES)-like structures, which has been previously reported in sequence type 59 (ST59) methicillin-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n            (MRSA). The remaining 8\n            <jats:italic>ermB<\/jats:italic>\n            -carrying isolates, belonging to ST7 (\n            <jats:italic>n<\/jats:italic>\n            = 4), ST5 (\n            <jats:italic>n<\/jats:italic>\n            = 3), and ST59 (\n            <jats:italic>n<\/jats:italic>\n            = 1), were\n            <jats:italic>sasK<\/jats:italic>\n            intact and did not carry MES-like structures. Unlike a MES-like structure that was located on the chromosome, the\n            <jats:italic>ermB<\/jats:italic>\n            elements on\n            <jats:italic>sasK<\/jats:italic>\n            -intact isolates were located on plasmids by S1 nuclease pulsed-field gel electrophoresis (PFGE) analysis and conjugation tests. Sequence data for the\n            <jats:italic>ermB<\/jats:italic>\n            -containing region (14,566 bp) from ST59 NTUH_3874 revealed that the best match was a Tn\n            <jats:italic>1546<\/jats:italic>\n            -like element in plasmid pMCCL2 DNA (GenBank accession number\n            <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.ncbi.nlm.nih.gov\/nucleotide\/AP009486\">AP009486<\/jats:ext-link>\n            ) of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Macrococcus caseolyticus<\/jats:named-content>\n            . Tn\n            <jats:italic>1546<\/jats:italic>\n            is recognized as an enterococcal transposon and was known from the vancomycin resistance gene cluster in vancomycin-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterococcus<\/jats:named-content>\n            (VRE). So far, acquisitions of Tn\n            <jats:italic>1546<\/jats:italic>\n            in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n            have occurred in clonal complex 5 (CC5) MRSA, but not in MSSA. This is the first report that MSSA harbors an\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterococcus faecium<\/jats:named-content>\n            -originated\n            <jats:italic>ermB<\/jats:italic>\n            -positive Tn\n            <jats:italic>1546<\/jats:italic>\n            -like element located on a plasmid.\n          <\/jats:p>","DOI":"10.1128\/aac.01096-16","type":"journal-article","created":{"date-parts":[[2016,8,2]],"date-time":"2016-08-02T03:04:34Z","timestamp":1470107074000},"page":"6108-6114","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Novel Structure of Enterococcus faecium-Originated\n            <i>ermB<\/i>\n            -Positive Tn\n            <i>1546<\/i>\n            -Like Element in Staphylococcus aureus"],"prefix":"10.1128","volume":"60","author":[{"given":"Tsai-Wen","family":"Wan","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Chun","family":"Hung","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Kaohsiung Medical University, Kaohsiung, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jui-Chang","family":"Tsai","sequence":"additional","affiliation":[{"name":"Institute of Medical Device and Imaging, National Taiwan University College of Medicine, Taipei, Taiwan"},{"name":"Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Tzu","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Po-Ren","family":"Hsueh","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan"},{"name":"Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tai-Fen","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan"},{"name":"Department of 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