{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:19:26Z","timestamp":1792109966942,"version":"4.4.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI45875"],"award-info":[{"award-number":["R01AI45875"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"HHS | NIH | National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["TL1 TR001428"],"award-info":[{"award-number":["TL1 TR001428"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n Urinary tract infections (UTIs) affect nearly 20% of women age 15 to 29 and account for an estimated $3.5 billion in costs. Antibiotic resistance prolongs UTI treatment, and resistance profiles vary regionally. This regional variation is an important consideration in guiding empirical treatment selection. Regional studies in the United States have identified tetracycline resistance in over one-third of\n Ureaplasma<\/jats:named-content>\n species isolates, but no studies have evaluated antibiotic resistance levels in college-aged women with a first-time UTI. We tested a panel of antibiotics and determined the MICs of\n Ureaplasma<\/jats:named-content>\n species (60\n U. parvum<\/jats:named-content>\n and 13\n U. urealyticum<\/jats:named-content>\n ) and 10\n Mycoplasma hominis<\/jats:named-content>\n isolates obtained from urine from college-aged women with a first-time UTI. Low antibiotic resistance was found in this population of women with a first-time UTI. All\n M. hominis<\/jats:named-content>\n and\n U. urealyticum<\/jats:named-content>\n isolates were sensitive. However, two\n U. parvum<\/jats:named-content>\n isolates were resistant, with one to levofloxacin (MIC, 4 \u03bcg\/ml) and one to tetracycline (MIC, 8 \u03bcg\/ml). For the\n Ureaplasma<\/jats:named-content>\n spp., the MIC\n 90<\/jats:sub>\n s were highest against gentamicin (21 \u03bcg\/ml) and lowest against doxycycline (0.25 \u03bcg\/ml). In a comparison of MIC levels between\n Ureaplasma<\/jats:named-content>\n spp.,\n U. urealyticum<\/jats:named-content>\n had significantly higher MICs against each antibiotic except doxycycline. For the resistant isolates, the genetic mechanisms of resistance were determined. PCR amplification identified\n tetM<\/jats:italic>\n to be present in the tetracycline-resistant isolate and an S83W mutation within the\n parC<\/jats:italic>\n gene of the quinolone-resistant isolate. To our knowledge, this study is the first to provide molecular and phenotypic evidence of the S83W\n parC<\/jats:italic>\n mutation conferring levofloxacin resistance in\n U. parvum<\/jats:named-content>\n isolated from a patient in the United States.\n <\/jats:p>","DOI":"10.1128\/aac.01104-17","type":"journal-article","created":{"date-parts":[[2017,8,22]],"date-time":"2017-08-22T00:20:21Z","timestamp":1503361221000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Antibacterial Resistance in Ureaplasma Species and Mycoplasma hominis Isolates from Urine Cultures in College-Aged Females"],"prefix":"10.1128","volume":"61","author":[{"given":"Marissa A.","family":"Valentine-King","sequence":"first","affiliation":[{"name":"Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary B.","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida, USA"},{"name":"Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"1","article-title":"Ambulatory medical care utilization estimates for 2007","volume":"13","author":"Schappert SM","year":"2011","unstructured":"Schappert SM RechtsteinerEA. 2011. 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