{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:01:05Z","timestamp":1762866065273},"reference-count":31,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2013,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n Pseudomonas aeruginosa<\/jats:named-content>\n NCGM1588 has a novel chromosomal class 1 integron, In151, which includes the\n aac(6\u2032)-Iaj<\/jats:italic>\n gene. The encoded protein, AAC(6\u2032)-Iaj, was found to consist of 184 amino acids, with 70% identity to AAC(6\u2032)-Ia.\n Escherichia coli<\/jats:named-content>\n transformed with a plasmid containing the\n aac(6\u2032)-Iaj<\/jats:italic>\n gene acquired resistance to all aminoglycosides tested except gentamicin. Of note,\n aac(6\u2032)-Iaj<\/jats:italic>\n contributed to the resistance to arbekacin. Thin-layer chromatography revealed that AAC(6\u2032)-Iaj acetylated all aminoglycosides tested except gentamicin. These findings indicated that AAC(6\u2032)-Iaj is a functional acetyltransferase that modifies the amino groups at the 6\u2032 positions of aminoglycosides and contributes to aminoglycoside resistance of\n P. aeruginosa<\/jats:named-content>\n NCGM1588, including arbekacin.\n <\/jats:p>","DOI":"10.1128\/aac.01105-12","type":"journal-article","created":{"date-parts":[[2012,10,16]],"date-time":"2012-10-16T05:51:17Z","timestamp":1350366677000},"page":"96-100","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Novel 6\u2032-\n N<\/i>\n -Aminoglycoside Acetyltransferase AAC(6\u2032)-Iaj from a Clinical Isolate of Pseudomonas aeruginosa"],"prefix":"10.1128","volume":"57","author":[{"given":"Tatsuya","family":"Tada","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tohru","family":"Miyoshi-Akiyama","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kayo","family":"Shimada","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Shimojima","sequence":"additional","affiliation":[{"name":"BML Inc., Kawagoe, Saitama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teruo","family":"Kirikae","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.57.1.138-163.1993"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.10.3196-3203.1992"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.3.430-450.2003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-7646(98)80026-5"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.3.528-534.1995"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.4.890"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.4.1053-1058.2002"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.1.342-349.2003"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.9.3734-3742.2005"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01772-06"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01360-08"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.06312-11"},{"key":"e_1_3_2_14_2","volume-title":"Approved standard M07-A9.","author":"Clinical and Laboratory Standards Institute","year":"2012","unstructured":"Clinical and Laboratory Standards Institute. 2012. 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