{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:48:53Z","timestamp":1791938933338,"version":"4.3.4"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T00:00:00Z","timestamp":1762300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T00:00:00Z","timestamp":1762300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2025,11,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                      experiments with\n                      <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                      showed that clofazimine exposure selected for delamanid and pretomanid resistance and mutations in\n                      <jats:italic toggle=\"yes\">fbiA<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">fbiC<\/jats:italic>\n                      , or\n                      <jats:italic toggle=\"yes\">fbiD<\/jats:italic>\n                      \u2014after the acquisition of\n                      <jats:italic toggle=\"yes\">Rv0678<\/jats:italic>\n                      mutations where this could be determined. Whether this is also possible\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      and in an\n                      <jats:italic toggle=\"yes\">Rv0678<\/jats:italic>\n                      wild-type background has to be studied further. Based on the available evidence, however, we propose that nitroimidazole resistance should not be considered an exclusion criterion for the use of clofazimine.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01113-25","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T12:00:21Z","timestamp":1758542421000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["<i>In vitro<\/i>\n                    exposure to clofazimine can select for delamanid and pretomanid resistance in\n                    <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1128","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9858-8935","authenticated-orcid":true,"given":"Praharshinie","family":"Rupasinghe","sequence":"first","affiliation":[{"name":"Unit of Mycobacteriology, Department of Biomedical Sciences, Institute of Tropical Medicine","place":["Antwerp, Belgium"]},{"name":"Belgian Coordinated Collections of Microorganisms \u2013 Mycobacterial collection (BCCM-ITM)","place":["Antwerp, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2287-1857","authenticated-orcid":true,"given":"Nabila","family":"Ismail","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bk57929","id-type":"ROR","asserted-by":"publisher"}],"name":"South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University","place":["Cape Town, South Africa"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9566-3653","authenticated-orcid":true,"given":"Wim","family":"Mulders","sequence":"additional","affiliation":[{"name":"Unit of Mycobacteriology, Department of Biomedical Sciences, Institute of Tropical Medicine","place":["Antwerp, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5741-7358","authenticated-orcid":true,"given":"Robin M.","family":"Warren","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bk57929","id-type":"ROR","asserted-by":"publisher"}],"name":"South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University","place":["Cape Town, South Africa"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0734-7149","authenticated-orcid":false,"given":"Lavania","family":"Joseph","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/007wwmx82","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for Tuberculosis, National Institute for Communicable Diseases, a division of the National Health Laboratory Services","place":["Johannesburg, South Africa"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9402-8615","authenticated-orcid":true,"given":"Dumisani","family":"Ngcamu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/007wwmx82","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for Tuberculosis, National Institute for Communicable Diseases, a division of the National Health Laboratory Services","place":["Johannesburg, South Africa"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7668-1178","authenticated-orcid":true,"given":"Thabisile","family":"Gwala","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/007wwmx82","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for Tuberculosis, National Institute for Communicable Diseases, a division of the National Health Laboratory Services","place":["Johannesburg, South Africa"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6983-1636","authenticated-orcid":false,"given":"Shaheed V.","family":"Omar","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/007wwmx82","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for Tuberculosis, National Institute for Communicable Diseases, a division of the National Health Laboratory Services","place":["Johannesburg, South Africa"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9181-7053","authenticated-orcid":true,"given":"Jens","family":"Vereecken","sequence":"additional","affiliation":[{"name":"Unit of Mycobacteriology, Department of Biomedical Sciences, Institute of Tropical Medicine","place":["Antwerp, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1017-4675","authenticated-orcid":true,"given":"Bouke C.","family":"de Jong","sequence":"additional","affiliation":[{"name":"Unit of Mycobacteriology, Department of Biomedical Sciences, Institute of Tropical Medicine","place":["Antwerp, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3301-9480","authenticated-orcid":true,"given":"Leen","family":"Rigouts","sequence":"additional","affiliation":[{"name":"Unit of Mycobacteriology, Department of Biomedical Sciences, Institute of Tropical Medicine","place":["Antwerp, Belgium"]},{"name":"Belgian Coordinated Collections of Microorganisms \u2013 Mycobacterial collection (BCCM-ITM)","place":["Antwerp, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6780-5119","authenticated-orcid":true,"given":"Emanuele","family":"Borroni","sequence":"additional","affiliation":[{"name":"Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute","place":["Milan, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6415-1535","authenticated-orcid":true,"given":"Daniela M.","family":"Cirillo","sequence":"additional","affiliation":[{"name":"Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute","place":["Milan, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Sch\u00f6n","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05ynxx418","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biomedical and Clinical Sciences, Division of Infection and Inflammation, Linkoping University","place":["Link\u00f6ping, Sweden"]},{"id":[{"id":"https:\/\/ror.org\/00pvxy857","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Infectious Diseases, Kalmar County Hospital, Link\u00f6ping University","place":["Kalmar, Sweden"]},{"id":[{"id":"https:\/\/ror.org\/05h1aye87","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Infectious Diseases, Link\u00f6ping University Hospital","place":["Link\u00f6ping, Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4610-2427","authenticated-orcid":true,"given":"Paolo","family":"Miotto","sequence":"additional","affiliation":[{"name":"Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute","place":["Milan, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0232-846X","authenticated-orcid":true,"given":"Claudio U.","family":"K\u00f6ser","sequence":"additional","affiliation":[{"name":"Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute","place":["Milan, Italy"]},{"id":[{"id":"https:\/\/ror.org\/05ynxx418","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biomedical and Clinical Sciences, Division of Infection and Inflammation, Linkoping University","place":["Link\u00f6ping, Sweden"]},{"id":[{"id":"https:\/\/ror.org\/013meh722","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Genetics, University of Cambridge","place":["Cambridge, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"WHO consolidated guidelines on tuberculosis: module 4: treatment and care","author":"World Health Organization","year":"2025","unstructured":"World Health Organization. 2025. WHO consolidated guidelines on tuberculosis: module 4: treatment and care. https:\/\/iris.who.int\/handle\/10665\/380799."},{"key":"e_1_3_3_3_2","volume-title":"Catalogue of mutations in Mycobacterium tuberculosis complex and their association with drug resistance, 2nd ed","author":"World Health Organization","year":"2023","unstructured":"World Health Organization. 2023. Catalogue of mutations in Mycobacterium tuberculosis complex and their association with drug resistance, 2nd ed. https:\/\/iris.who.int\/handle\/10665\/374061."},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008287"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-37184-7"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39300-z"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202400176"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01368-22"},{"key":"e_1_3_3_9_2","volume-title":"WHO operational handbook on tuberculosis: module 3: diagnosis: rapid diagnostics for tuberculosis detection: web annex C: technical manual for culture-based drug susceptibility testing of anti-tuberculosis drugs used in the treatment of tuberculosis, 3rd ed","author":"World Health Organization","year":"2024","unstructured":"World Health Organization. 2024. WHO operational handbook on tuberculosis: module 3: diagnosis: rapid diagnostics for tuberculosis detection: web annex C: technical manual for culture-based drug susceptibility testing of anti-tuberculosis drugs used in the treatment of tuberculosis, 3rd ed. https:\/\/iris.who.int\/handle\/10665\/376286."},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-36955-y"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2024.12.035"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00344-18"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00070-24"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00946-24"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12127"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.00326-24"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01304-20"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkac070"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00166-2022"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkae041"},{"key":"e_1_3_3_21_2","volume-title":"Catalogue of mutations in Mycobacterium tuberculosis complex and their association with drug resistance","author":"World Health Organization","year":"2021","unstructured":"World Health Organization. 2021. Catalogue of mutations in Mycobacterium tuberculosis complex and their association with drug resistance. https:\/\/iris.who.int\/handle\/10665\/341981."},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(21)00301-3"},{"key":"e_1_3_3_23_2","volume-title":"Technical report on critical concentrations for drug susceptibility testing of isoniazid and the rifamycins (rifampicin, rifabutin and rifapentine)","author":"World Health Organization","year":"2021","unstructured":"World Health Organization. 2021. Technical report on critical concentrations for drug susceptibility testing of isoniazid and the rifamycins (rifampicin, rifabutin and rifapentine). https:\/\/iris.who.int\/handle\/10665\/339275."},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01096-23"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgph.0002283"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2025.102630"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.01113-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.01113-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T23:06:57Z","timestamp":1785884817000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aac.01113-25"}},"subtitle":[],"editor":[{"given":"Sean","family":"Wasserman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,11,5]]},"references-count":25,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11,5]]}},"alternative-id":["10.1128\/aac.01113-25"],"URL":"https:\/\/doi.org\/10.1128\/aac.01113-25","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,5]]},"assertion":[{"value":"2025-07-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01113-25"}}