{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T02:39:33Z","timestamp":1771900773148,"version":"3.50.1"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Trypanozoon<\/jats:named-content>\n            parasites infect both humans, causing sleeping sickness, and animals, causing nagana, surra, and dourine. Control of nagana and surra depends to a great extent on chemotherapy. However, drug resistance to several of the front-line drugs is rising. Furthermore, there is no official treatment for dourine. Therefore, there is an urgent need to develop antiparasitic agents with novel modes of action. Host defense peptides have recently gained attention as promising candidates. We have previously reported that one such peptide, the equine antimicrobial peptide eCATH1, is highly active against equine Gram-positive and Gram-negative bacteria, without cytotoxicity against mammalian cells at bacteriolytic concentrations. In the present study, we show that eCATH1 exhibits an\n            <jats:italic>in vitro<\/jats:italic>\n            50% inhibitory concentration (IC\n            <jats:sub>50<\/jats:sub>\n            ) of 9.5 \u03bcM against\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Trypanosoma brucei brucei<\/jats:named-content>\n            ,\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Trypanosoma evansi<\/jats:named-content>\n            , and\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Trypanosoma equiperdum<\/jats:named-content>\n            . Its trypanocidal mechanism involves plasma membrane permeabilization and mitochondrial alteration based on the following data: (i) eCATH1 induces the rapid influx of the vital dye SYTOX Green; (ii) it rapidly disrupts mitochondrial membrane potential, as revealed by immunofluorescence microscopy using the fluorescent dye rhodamine 123; (iii) it severely damages the membrane and intracellular structures of the parasites as early as 15 min after exposure at 9.5 \u03bcM and 5 min after exposure at higher concentrations (19 \u03bcM), as evidenced by scanning and transmission electron microscopy. We also demonstrate that administration of eCATH1 at a dose of 10 mg\/kg to\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">T. equiperdum<\/jats:named-content>\n            -infected mice delays mortality. Taken together, our findings suggest that eCATH1 is an interesting template for the development of novel therapeutic agents in the treatment of trypanosome infections.\n          <\/jats:p>","DOI":"10.1128\/aac.01127-15","type":"journal-article","created":{"date-parts":[[2016,1,12]],"date-time":"2016-01-12T03:49:28Z","timestamp":1452570568000},"page":"2610-2619","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Killing of Trypanozoon Parasites by the Equine Cathelicidin eCATH1"],"prefix":"10.1128","volume":"60","author":[{"given":"S.","family":"Cauchard","sequence":"first","affiliation":[{"name":"ANSES, Dozule Laboratory for Equine Diseases, Bacteriology and Parasitology Unit, Goustranville, France"},{"name":"Universit\u00e9 de Caen Basse-Normandie, EA4655 U2RM (\u00c9quipe Antibio-R\u00e9sistance), Caen, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Van Reet","sequence":"additional","affiliation":[{"name":"Unit of Parasite Diagnostics, Department of Biomedical Sciences, Institute for Tropical Medicine, Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"B\u00fcscher","sequence":"additional","affiliation":[{"name":"Unit of Parasite Diagnostics, Department of Biomedical Sciences, Institute for Tropical Medicine, Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Goux","sequence":"additional","affiliation":[{"name":"Centre de Microscopie Appliqu\u00e9e \u00e0 la Biologie, SF4206 ICORE, Universit\u00e9 de Caen Basse-Normandie, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Gr\u00f6tzinger","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Christian-Albrechts University of Kiel, Kiel, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Leippe","sequence":"additional","affiliation":[{"name":"Zoological Institute, Comparative Immunobiology, Christian-Albrechts University of Kiel, Kiel, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Cattoir","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Caen Basse-Normandie, EA4655 U2RM (\u00c9quipe Antibio-R\u00e9sistance), Caen, France"},{"name":"CHU de Caen, Service de Microbiologie, Caen, France"},{"name":"CNR de la R\u00e9sistance aux Antibiotiques, Laboratoire Associ\u00e9 Ent\u00e9rocoques, Caen, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Laugier","sequence":"additional","affiliation":[{"name":"ANSES, Dozule Laboratory for Equine Diseases, Bacteriology and Parasitology Unit, Goustranville, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Cauchard","sequence":"additional","affiliation":[{"name":"ANSES, Dozule Laboratory for Equine Diseases, Bacteriology and Parasitology Unit, Goustranville, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"OIE-listed diseases, infections and infestations in force in 2015","author":"World Organisation for Animal Health (OIE)","year":"2015","unstructured":"World Organisation for Animal Health (OIE). 28 April 2015. 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