{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T23:23:25Z","timestamp":1791933805770,"version":"4.3.4"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100008578","name":"Georgia Regents University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008578","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Molecular characterization of\n            <jats:italic>cyp51A<\/jats:italic>\n            from the azole-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Aspergillus fumigatus<\/jats:named-content>\n            isolate 50593 from a lung transplant patient showed Y121F\/T289A changes coupled with a 46-bp tandem repeat (TR46) on the promoter, whereas\n            <jats:italic>cyp51A<\/jats:italic>\n            from the pretherapy isolate,\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. fumigatus<\/jats:named-content>\n            47381, showed no changes. This is the first reported case of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. fumigatus<\/jats:named-content>\n            azole resistance due to Y121F\/T289A\/TR46 in the United States, suggesting that multiple mutational alterations of\n            <jats:italic>cyp51A<\/jats:italic>\n            resulting in high-level azole resistance could occur during prolonged antifungal therapy.\n          <\/jats:p>","DOI":"10.1128\/aac.01130-15","type":"journal-article","created":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T02:33:37Z","timestamp":1447727617000},"page":"1129-1133","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Molecular Characterization of a Voriconazole-Resistant, Posaconazole-Susceptible Aspergillus fumigatus Isolate in a Lung Transplant Recipient in the United States"],"prefix":"10.1128","volume":"60","author":[{"given":"Jose A.","family":"Vazquez","sequence":"first","affiliation":[{"name":"Section of Infectious Diseases, Department of Medicine, Georgia Regents University, Augusta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elias K.","family":"Manavathu","sequence":"additional","affiliation":[{"name":"Section of Infectious Diseases, Department of Medicine, Georgia Regents University, Augusta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0050219"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(09)70265-8"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1507.090043"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.3.1120-1124.2003"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.5.1719-1726.2003"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.7.2747-2750.2004"},{"key":"e_1_3_2_8_2","first-page":"929","volume-title":"Antimicrobial drug resistance","author":"Chandrasekar PH","year":"2009","unstructured":"Chandrasekar PH, Manavathu EK. 2009. Antifungal resistance: Aspergillus, p 929\u2013941. In Mayers DL (ed), Antimicrobial drug resistance. Humana Press, New York, NY."},{"key":"e_1_3_2_9_2","volume-title":"Abstr 43rd Intersci Conf Antimicrob Agents Chemother","author":"Manavathu EK","year":"2003","unstructured":"Manavathu EK, Baskaran I, Alangaden GJ, Chandrasekar PH. 2003. Cytochrome P450 14alpha-sterol demethylase mutation dependent triazole cross-resistance in Aspergillus fumigatus, abstr M-471. Abstr 43rd Intersci Conf Antimicrob Agents Chemother. American Society for Microbiology, Washington, DC."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01092-06"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku364"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.7.2431-2438.2001"},{"key":"e_1_3_2_13_2","volume-title":"CLSI document M38-A2","author":"Clinical and Laboratory Standards Institute","year":"2008","unstructured":"Clinical and Laboratory Standards Institute. 2008. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; approved standard\u20142nd ed. CLSI document M38-A2. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1358\/dot.2002.38.12.820101"},{"key":"e_1_3_2_15_2","volume-title":"DNeasy plant or fungi DNA isolation handbook","author":"Qiagen, Inc","year":"2012","unstructured":"Qiagen, Inc. 2012. 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