{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T21:57:35Z","timestamp":1790114255171,"version":"4.0.1"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A novel \u03b2-lactamase gene was cloned from the whole-cell DNA of an enterobacterial\n            <jats:italic>Citrobacter gillenii<\/jats:italic>\n            reference strain that displayed a weak narrow-spectrum \u03b2-lactam-resistant phenotype and was expressed in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . It encoded a clavulanic acid-inhibited Ambler class A \u03b2-lactamase, GIL-1, with a pI value of 7.5 and a molecular mass of ca. 29 kDa. GIL-1 had the highest percent amino acid sequence identity with TEM-1 and SHV-1, 77%, and 67%, respectively, and only 46%, 31%, and 32% amino acid sequence identity with CKO-1 (\n            <jats:italic>C. koseri<\/jats:italic>\n            ), CdiA1 (\n            <jats:italic>C. diversus<\/jats:italic>\n            ), and SED-1 (\n            <jats:italic>C. sedlaki<\/jats:italic>\n            ), respectively. The substrate profile of the purified GIL-1 was similar to that of \u03b2-lactamases TEM-1 and SHV-1. The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>GIL-1<\/jats:sub>\n            gene was chromosomally located, as revealed by I-CeuI experiments, and was constitutively expressed at a low level in\n            <jats:italic>C. gillenii<\/jats:italic>\n            . No gene homologous to the regulatory\n            <jats:italic>ampR<\/jats:italic>\n            genes of chromosomal class C \u03b2-lactamases was found upstream of the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>GIL-1<\/jats:sub>\n            gene, which fits the noninducibility of \u03b2-lactamase expression in\n            <jats:italic>C. gillenii<\/jats:italic>\n            . Rapid amplification of DNA 5\u2032 ends analysis of the promoter region revealed putative promoter sequences that diverge from what has been identified as the consensus sequence in\n            <jats:italic>E. coli<\/jats:italic>\n            . The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>GIL-1<\/jats:sub>\n            gene was part of a 5.5-kb DNA fragment bracketed by a 9-bp duplication and inserted between the\n            <jats:sc>d<\/jats:sc>\n            -lactate dehydrogenase gene and the\n            <jats:italic>ydbH<\/jats:italic>\n            genes; this DNA fragment was absent in other\n            <jats:italic>Citrobacter<\/jats:italic>\n            species. This work further illustrates the heterogeneity of \u03b2-lactamases in\n            <jats:italic>Citrobacter<\/jats:italic>\n            spp., which may indicate that the variability of\n            <jats:italic>Citrobacter<\/jats:italic>\n            species is greater than expected.\n          <\/jats:p>","DOI":"10.1128\/aac.01152-06","type":"journal-article","created":{"date-parts":[[2007,1,23]],"date-time":"2007-01-23T01:48:47Z","timestamp":1169516927000},"page":"1365-1372","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Chromosome-Encoded Narrow-Spectrum Ambler Class A \u03b2-Lactamase GIL-1 from\n            <i>Citrobacter gillenii<\/i>"],"prefix":"10.1128","volume":"51","author":[{"given":"Thierry","family":"Naas","sequence":"first","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique-Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Aubert","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique-Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayla","family":"O\u0308zcan","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique-Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, Ho\u0302pital de Bice\u0302tre, Assistance Publique-Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine Paris-Sud, 94275 Le Kremlin-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.1924-1930.1997"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh151"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.8.2619-2624.1999"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-43-4-645"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.18.4196"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_9_2","unstructured":"Clinical and Laboratory Standards Institute. 2005. 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