{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:21:42Z","timestamp":1791354102264,"version":"4.3.1"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Triclosan, a very widely used biocide, specifically inhibits fatty acid synthesis by inhibition of enoyl-acyl carrier protein (ACP) reductase.\n            <jats:italic>Escherichia coli<\/jats:italic>\n            FabI is the prototypical triclosan-sensitive enoyl-ACP reductase, and\n            <jats:italic>E. coli<\/jats:italic>\n            is extremely sensitive to the biocide. However, other bacteria are resistant to triclosan, because they encode triclosan-resistant enoyl-ACP reductase isozymes. In contrast, the triclosan resistance of\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            PAO1 has been attributed to active efflux of the compound (R. Chuanchuen, R. R. Karkhoff-Schweizer, and H. P. Schweizer, Am. J. Infect. Control\n            <jats:bold>31:<\/jats:bold>\n            124-127, 2003). We report that\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            contains two enoyl-ACP reductase isozymes, the previously characterized FabI homologue plus a homologue of FabV, a triclosan-resistant enoyl-ACP reductase recently demonstrated in\n            <jats:italic>Vibrio cholerae<\/jats:italic>\n            . By deletion of the genes encoding\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            FabI and FabV, we demonstrated that FabV confers triclosan resistance on\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            . Upon deletion of the\n            <jats:italic>fabV<\/jats:italic>\n            gene, the mutant strain became extremely sensitive to triclosan (&gt;2,000-fold more sensitive than the wild-type strain), whereas the mutant strain lacking FabI remained completely resistant to the biocide.\n          <\/jats:p>","DOI":"10.1128\/aac.01152-09","type":"journal-article","created":{"date-parts":[[2009,11,24]],"date-time":"2009-11-24T03:44:44Z","timestamp":1259034284000},"page":"689-698","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":187,"title":["Triclosan Resistance of\n            <i>Pseudomonas aeruginosa<\/i>\n            PAO1 Is Due to FabV, a Triclosan-Resistant Enoyl-Acyl Carrier Protein Reductase"],"prefix":"10.1128","volume":"54","author":[{"given":"Lei","family":"Zhu","sequence":"first","affiliation":[{"name":"College of Life Science"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinshui","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Life Science"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jincheng","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Life Science"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John E.","family":"Cronan","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry and Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haihong","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Science"},{"name":"Key Laboratory of Animal Disease Control and Prevention of the Ministry of Agriculture of China, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3091029"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-138-10-2093"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)37485-9"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2005.04.020"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1067\/mic.2003.11"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00294a032"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.2144\/98255bm04"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.7.2710-2715.1998"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2007.2045"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00640.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.61.4.429-441.1997"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(90)90339-Z"},{"key":"e_1_3_2_14_2","first-page":"448","volume":"33","year":"2004","unstructured":"Guill\u00e9n, J., A. 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