{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T02:59:54Z","timestamp":1780369194946,"version":"3.54.1"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"The CAMS Innovation Fund for Medical Sciences","award":["2021-I2M-1-038"],"award-info":[{"award-number":["2021-I2M-1-038"]}]},{"name":"The Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences","award":["2019PT310029,2023-PT310-04"],"award-info":[{"award-number":["2019PT310029,2023-PT310-04"]}]},{"name":"The National Science and Technology Infrastructure of China","award":["Project No. National Pathogen Resource Center-NPRC-32"],"award-info":[{"award-number":["Project No. National Pathogen Resource Center-NPRC-32"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2024,12,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Cephalosporin resistance in\n              <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n              has severely compromised the efficacy of World Health Organization (WHO)-recommended therapies. This study aimed to methodologically evaluate the optimized Six-Codon\n              <jats:sup>Plus<\/jats:sup>\n              assay, and additionally conducted a multicenter evaluation to assess its clinical application, especially for predicting antimicrobial resistance (AMR). For methodological evaluation, 397 sequence-known\n              <jats:italic>N. gonorrhoeae<\/jats:italic>\n              isolates were evaluated for specificity, 17 nongonococcal isolates were assessed for cross-reactivity, 159 uncultured urogenital swabs and urine samples were evaluated for sensitivity at the clinical level. For multicenter evaluation, 773 isolates with confirmed phenotypic data and 718 clinical urogenital swabs collected from four geographical cities were, respectively, utilized for the evaluation of AMR-prediction strategies and the clinical application of the assay. The assay accurately identified specific single-nucleotide polymorphisms in resistance-associated genes, the detection limits dropped to 10 copies\/reaction for individual targets. The specificity reached 100% and no cross-reactivity occurred with double-target confirmation. The assay could be directly applied to clinical samples containing over 20 copies\/reaction. Multicenter evaluation formulated two optimal strategies for decreased susceptibility prediction in specific scenarios, and one tactic for prediction of resistance and identification of FC428-like strains. High sensitivity of 86.84% (95% CI, 71.11\u201395.05) and specificity of 99.59% (95% CI, 98.71\u201399.89) for resistance prediction were demonstrated for ceftriaxone (CRO). Regarding\n              <jats:italic>N. gonorrhoeae<\/jats:italic>\n              identification among multicenter swabs, specificity reached 97.53% (95% CI, 95.49\u201398.69), and sensitivity reached 93.77% (95% CI, 90.04\u201396.22). The Six-Codon\n              <jats:sup>Plus<\/jats:sup>\n              assay exhibited excellent detection performance and formulated optimal AMR-related prediction strategy with regional adaptability, providing critical information for population screening and clinical treatment.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.01165-24","type":"journal-article","created":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T13:00:38Z","timestamp":1730206838000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Multigeographic clinical assessment of a molecular diagnostic assay for detection of key codons to predict decreased susceptibility or resistance to cephalosporins in\n            <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1128","volume":"68","author":[{"given":"Liqin","family":"Wang","sequence":"first","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College","place":["Beijing, China"]},{"name":"Key Laboratory of Respiratory Disease Pathogenomics, Chinese Academy of Medical Sciences & Peking Union Medical College","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China","place":["Hefei, China"]},{"name":"Core Unit of National Clinical Research Center for Laboratory Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leshan","family":"Xiu","sequence":"additional","affiliation":[{"name":"School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine","place":["Shanghai, China"]},{"name":"One Health Center, Shanghai Jiao Tong University-The University of Edinburgh","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Hu","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Institute of Dermatology","place":["Deqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Huang","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Institute of Dermatology","place":["Deqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Yong","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People\u2019s Hospital, University of Electronic Science and Technology of China","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People\u2019s Hospital, University of Electronic Science and Technology of China","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenling","family":"Cao","sequence":"additional","affiliation":[{"name":"Guangzhou Institute of Dermatology","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanghai Skin Disease Hospital, Tongji University School of Medicine","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Chronic 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