{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T18:35:44Z","timestamp":1789583744081,"version":"build-2803163510"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2007,7,1]],"date-time":"2007-07-01T00:00:00Z","timestamp":1183248000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The in vitro susceptibility of\n            <jats:italic>Sporothrix schenckii<\/jats:italic>\n            to antifungal drugs has been determined with three different methods. Nineteen Peruvian clinical isolates of\n            <jats:italic>S. schenckii<\/jats:italic>\n            were tested against amphotericin B (AB), flucytosine (FC), fluconazole (FZ), itraconazole (IZ), voriconazole (VZ), and ketoconazole (KZ). Modified NCCLS M38-A, Sensititre YeastOne (SYO), and ATB Fungus 2 (ATBF2) methods were used to determine the MICs. ATCC isolates of\n            <jats:italic>Candida parapsilosis<\/jats:italic>\n            ,\n            <jats:italic>Candida krusei<\/jats:italic>\n            , and\n            <jats:italic>Aspergillus flavus<\/jats:italic>\n            were used for quality control.\n            <jats:italic>Sporothrix<\/jats:italic>\n            inocula were prepared with the mycelial form growing on potato dextrose agar at 28 \u00b1 2\u00b0C. MICs of AB, FC, FZ, and IZ were determined with all three methods, VZ with M38-A and SYO, and KZ with only SYO. The three methods showed high MICs of FZ and FC (MIC\n            <jats:sub>90<\/jats:sub>\n            of 0.5 \u03bcg\/ml), being homogeneously lower than those of IZ and KZ. The M38-A method showed a variable MIC range of VZ (4.0 to 16 \u03bcg\/ml); the geometric mean (GM) was 9.3 \u03bcg\/ml. The MIC range of AB was wide (0.06 to 16 \u03bcg\/ml), but the GM was 1.2 \u03bcg\/ml, suggesting that the MIC is strain dependent. Agreement (two log\n            <jats:sub>2<\/jats:sub>\n            dilutions) between commercial techniques and the modified M38-A method was very high with FZ, IZ, and FC. In AB and VZ, the agreement was lower, being related to the antifungal concentrations of each method. The highest activity against\n            <jats:italic>S. schenckii<\/jats:italic>\n            was found with IZ and KZ. Lack of activity was observed with FZ, VZ, and FC. When AB is indicated for sporotrichosis, the susceptibility of the strain must be analyzed. Commercial quantitative antifungal methods have a limited usefulness in\n            <jats:italic>S. schenckii<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.01176-06","type":"journal-article","created":{"date-parts":[[2007,4,17]],"date-time":"2007-04-17T00:38:55Z","timestamp":1176770335000},"page":"2420-2423","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["In Vitro Susceptibility of\n            <i>Sporothrix schenckii<\/i>\n            to Six Antifungal Agents Determined Using Three Different Methods"],"prefix":"10.1128","volume":"51","author":[{"given":"Eidi","family":"Alvarado-Rami\u0301rez","sequence":"first","affiliation":[{"name":"Infectious Diseases and Mycology Research Unit (URMIM), Institut Municipal d'Investigacio\u0301 Me\u0300dica (IMIM), Faculty of Medicine, Universitat Auto\u0300noma de Barcelona, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josep M.","family":"Torres-Rodri\u0301guez","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Mycology Research Unit (URMIM), Institut Municipal d'Investigacio\u0301 Me\u0300dica (IMIM), Faculty of Medicine, Universitat Auto\u0300noma de Barcelona, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"1279","volume":"113","year":"1989","unstructured":"Baker, J. H., H. C. Goodpasture, H. R. Kuhns, and M. G. Rinaldi. 1989. Fungemia caused by an amphotericin B-resistant isolate of Sporotrhix schenckii. Successful treatment with itraconazole. Arch. Pathol. Lab. Med.113:1279-1281.","journal-title":"Arch. Pathol. Lab. Med."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.2.5.402"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/13.1.47"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1139\/m97-169"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.1995.tb00067.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/49.suppl_1.7"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010954803886"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.1.198-202.1998"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.2.314"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/313751"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/21.4.981"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1988.01060130402030"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg384"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1080\/mmy.39.4.369.371"},{"key":"e_1_3_2_16_2","first-page":"103","volume":"22","year":"2005","unstructured":"Morera-Lopez, Y., J. M. Torres-Rodr\u00edguez, and T. Jim\u00e9nez-Cabello. 2005. Estudio de la sensibilidad in vitro de aislamientos cl\u00ednicos de mohos y levaduras a itraconazol y voriconazol. Rev. Iberoam. Micol.22:103-107.","journal-title":"Rev. Iberoam. Micol."},{"key":"e_1_3_2_17_2","unstructured":"Reference method for broth dilution antifungal susceptibility testing for yeasts 2002"},{"key":"e_1_3_2_18_2","unstructured":"Reference methods for broth dilution antifungal susceptibility testing of filamentous fungi 2002"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.5.1912-1915.2004"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.7.2618-2621.2002"},{"key":"e_1_3_2_21_2","first-page":"323","year":"1998","unstructured":"Schell, W. A. 1998. Agents of chromoblastomycosis and sporotrichosis, p. 323-336. In L. Ajello and R. J. Hay (ed.), Topley & Wilson's Medical Mycology, 9th ed. Arnold Press, London, United Kingdom.","journal-title":"Topley & Wilson's Medical Mycology"},{"key":"e_1_3_2_22_2","first-page":"294","volume":"42","year":"2006","unstructured":"Skrodeniene, E., A. Dambrauskiene, and A. Vitkausikene. 2006. Susceptibility of yeast to antifungal agents in Kaunas University of Medicine Hospital. Medicina (Kaunas)42:294-299.","journal-title":"Medicina (Kaunas)"},{"key":"e_1_3_2_23_2","first-page":"350","volume":"40","year":"2000","unstructured":"Ware, A. J., C. J. Cockerell, D. J. Skiest, and H. M. Kussman. 2000. Disseminated sporotrichosis with extensive cutaneous involvement in a patient with AIDS. J. Am. Acad. Dermatol.40:350-355.","journal-title":"J. Am. Acad. Dermatol."},{"key":"e_1_3_2_24_2","first-page":"1492","year":"2000","unstructured":"Weeke, J. 2000. Thyroid and antithyroid drugs, p. 1492-1493. In M. N. G. Dukes and J. K. Aronson (ed.), Meyler's side effects of drugs, 14th ed. Elsevier Press, Amsterdam, The Netherlands.","journal-title":"Meyler's side effects of drugs"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/17.2.210"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01176-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01176-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T19:23:17Z","timestamp":1645471397000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01176-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,7]]},"references-count":24,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2007,7]]}},"alternative-id":["10.1128\/AAC.01176-06"],"URL":"https:\/\/doi.org\/10.1128\/aac.01176-06","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,7]]},"assertion":[{"value":"2006-09-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-04-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}