{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:47:27Z","timestamp":1789519647328,"version":"build-2803163510"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["ZIA CL090088"],"award-info":[{"award-number":["ZIA CL090088"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,2,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Aspergillus<\/jats:italic>\n                      infection poses a major clinical challenge, particularly in immunocompromised individuals, with invasive diseases associated with high mortality. While\n                      <jats:italic toggle=\"yes\">Aspergillus fumigatus<\/jats:italic>\n                      remains the predominant species causing human infections, recent studies highlight the growing clinical significance of lesser-known and cryptic\n                      <jats:italic toggle=\"yes\">Aspergillus<\/jats:italic>\n                      species, which often exhibit reduced susceptibility to standard antifungal therapies. In this study, we analyzed 196 clinical\n                      <jats:italic toggle=\"yes\">Aspergillus<\/jats:italic>\n                      isolates from 107 patients treated at the NIH Clinical Center between 2019 and 2022. A total of 38\n                      <jats:italic toggle=\"yes\">Aspergillus<\/jats:italic>\n                      species across 11 taxonomic sections were identified, with non-\n                      <jats:italic toggle=\"yes\">fumigatus<\/jats:italic>\n                      and cryptic species accounting for 77.1% of all isolates. The most frequently recovered species were\n                      <jats:italic toggle=\"yes\">A. fumigatus sensu stricto<\/jats:italic>\n                      (22.9%),\n                      <jats:italic toggle=\"yes\">A. sydowii<\/jats:italic>\n                      (8.7%),\n                      <jats:italic toggle=\"yes\">A. calidoustus<\/jats:italic>\n                      (7.1%),\n                      <jats:italic toggle=\"yes\">A. nidulans<\/jats:italic>\n                      (6.6%),\n                      <jats:italic toggle=\"yes\">A. tanneri<\/jats:italic>\n                      (6.1%), and\n                      <jats:italic toggle=\"yes\">A. terreus<\/jats:italic>\n                      (5.6%). Species-level identification was achieved in 43% of isolates using matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS). In contrast, PCR sequencing confirmed species identity in over 88% of isolates by targeting the internal transcribed spacer (ITS) region of rDNA, 81% using the \u03b2-tubulin gene, and 68% using the calmodulin gene. The most common underlying clinical conditions among patients were bronchiectasis (35%), chronic granulomatous disease (22%), and pulmonary non-tuberculous mycobacterial infection (17%). Out of 107 patients, eight died (8\/107, 7.5%); six of these deaths occurred in patients with chronic granulomatous disease (CGD) and two in patients with RAG1 deficiency. Antifungal susceptibility testing showed that olorofim had the lowest minimal inhibitory concentrations across species. In contrast, the activity of triazoles and amphotericin B was variable, particularly against\n                      <jats:italic toggle=\"yes\">A. tanneri<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">A. calidoustus<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">A. sydowii<\/jats:italic>\n                      . This study presents one of the largest species-level data sets of\n                      <jats:italic toggle=\"yes\">Aspergillus<\/jats:italic>\n                      isolates to date, underscoring the diversity, pathogenic potential, and resistance profiles of non-\n                      <jats:italic toggle=\"yes\">fumigatus<\/jats:italic>\n                      species. Accurate species identification plays an important role in guiding appropriate antifungal therapy and improving clinical outcomes, although further studies are needed to elucidate its direct impact on treatment decisions.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01184-25","type":"journal-article","created":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T14:52:40Z","timestamp":1768315960000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Beyond\n                    <i>Fumigatus<\/i>\n                    : a molecular portrait of clinical\n                    <i>Aspergillus<\/i>\n                    diversity, pathogenicity, and antifungal resistance"],"prefix":"10.1128","volume":"70","author":[{"given":"Chioma 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