{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T02:40:01Z","timestamp":1778121601779,"version":"3.51.4"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Corynebacterium striatum<\/jats:named-content>\n            BM4687 was resistant to gentamicin and tobramycin but susceptible to kanamycin A and amikacin, a phenotype distinct among Gram-positive bacteria. Analysis of the entire genome of this strain did not detect any genes for known aminoglycoside resistance enzymes. Yet, annotation of the coding sequences identified 12 putative acetyltransferases or GCN5-related\n            <jats:italic>N<\/jats:italic>\n            -acetyltransferases. A total of 11 of these coding sequences were also present in the genomes of other\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Corynebacterium<\/jats:named-content>\n            spp. The 12th coding sequence had 55 to 60% amino acid identity with acetyltransferases in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Actinomycetales<\/jats:named-content>\n            . The gene was cloned in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n            , where it conferred resistance to aminoglycosides by acetylation. The protein was purified to homogeneity, and its steady-state kinetic parameters were determined for dibekacin and kanamycin B. The product of the turnover of dibekacin was purified, and its structure was elucidated by high-field nuclear magnetic resonance (NMR), indicating transfer of the acetyl group to the amine at the C-3 position. Due to the unique profile of the reaction, it was designated aminoglycoside 3-\n            <jats:italic>N<\/jats:italic>\n            -acetyltransferase type XI.\n          <\/jats:p>","DOI":"10.1128\/aac.01203-15","type":"journal-article","created":{"date-parts":[[2015,7,7]],"date-time":"2015-07-07T02:29:57Z","timestamp":1436236197000},"page":"5647-5653","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["AAC(3)-XI, a New Aminoglycoside 3-\n            <i>N<\/i>\n            -Acetyltransferase from Corynebacterium striatum"],"prefix":"10.1128","volume":"59","author":[{"given":"Marc","family":"Galimand","sequence":"first","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 des Agents Antibact\u00e9riens, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Fishovitz","sequence":"additional","affiliation":[{"name":"University of Notre Dame, Notre Dame, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thierry","family":"Lambert","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de Pharmacie, Universit\u00e9 Paris Sud, Ch\u00e2tenay-Malabry, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val\u00e9rie","family":"Barbe","sequence":"additional","affiliation":[{"name":"C.E.A. Institut de G\u00e9nomique-Genoscope, Laboratoire de Biologie Mol\u00e9culaire pour l'Etude des G\u00e9nomes (LBioMEG), Evry, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaroslav","family":"Zajicek","sequence":"additional","affiliation":[{"name":"University of Notre Dame, Notre Dame, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahriar","family":"Mobashery","sequence":"additional","affiliation":[{"name":"University of Notre Dame, Notre Dame, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrice","family":"Courvalin","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 des Agents Antibact\u00e9riens, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2004.05.005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/23.6.1246"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/nmi2.48"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/PL00008668"},{"key":"e_1_3_2_6_2","volume-title":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically","author":"Clinical and Laboratory Standards Institute","year":"2015","unstructured":"Clinical and Laboratory Standards Institute. 2015. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. A07-A10. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1194"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh340"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1080\/10635150390235520"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03355.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(75)43124-X"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi060723g"},{"key":"e_1_3_2_13_2","first-page":"501","article-title":"An improved method for heteronuclear chemical shift correlation by two-dimensional NMR","volume":"42","author":"Bax A","year":"1981","unstructured":"Bax A, Morris GA. 1981. An improved method for heteronuclear chemical shift correlation by two-dimensional NMR. 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