{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T01:48:47Z","timestamp":1778636927398,"version":"3.51.4"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:00:00Z","timestamp":1519862400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100004737","name":"National Health Research Institutes","doi-asserted-by":"publisher","award":["IV-104-PP-15"],"award-info":[{"award-number":["IV-104-PP-15"]}],"id":[{"id":"10.13039\/501100004737","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004737","name":"National Health Research Institutes","doi-asserted-by":"publisher","award":["IV-105-PP-01"],"award-info":[{"award-number":["IV-105-PP-01"]}],"id":[{"id":"10.13039\/501100004737","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["103-2314-B-400-020-MY2"],"award-info":[{"award-number":["103-2314-B-400-020-MY2"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["105-2628-B-400-002-MY2"],"award-info":[{"award-number":["105-2628-B-400-002-MY2"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["105-2320-B-038-011"],"award-info":[{"award-number":["105-2320-B-038-011"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The rate of recovery of carbapenem-resistant\n            <jats:named-content content-type=\"genus-species\">Acinetobacter baumannii<\/jats:named-content>\n            (CRAB) isolates has increased significantly in recent decades in Taiwan. This study investigated the molecular epidemiology of CRAB with a focus on the mechanisms of resistance and spread in isolates with\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-23-like<\/jats:sub>\n            or\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-24-like<\/jats:sub>\n            . All 555 CRAB isolates in our multicenter collection, which were recovered from 2002 to 2010, were tested for the presence of class A, B, and D carbapenemase genes. All isolates with\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-23-like<\/jats:sub>\n            or\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-24-like<\/jats:sub>\n            were subjected to pulsed-field gel electrophoresis, and 82 isolates (60 isolates with\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-23-like<\/jats:sub>\n            and 22 isolates with\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-24-like<\/jats:sub>\n            ) were selected for multilocus sequence typing to determine the sequence type (ST) and clonal group (CG) and for detection of additional \u03b2-lactamase and aminoglycoside resistance genes. The flanking regions of carbapenem and aminoglycoside resistance genes were identified by PCR mapping and sequencing. The localization of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA<\/jats:sub>\n            was determined by S1 nuclease and I-CeuI assays. The numbers of CRAB isolates carrying\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-23-like<\/jats:sub>\n            or\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-24-like<\/jats:sub>\n            , especially those carrying\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-23-like<\/jats:sub>\n            , increased significantly from 2008 onward. The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-23-like<\/jats:sub>\n            gene was carried by antibiotic resistance genomic island 1 (AbGRI1)-type structures located on plasmids and\/or the chromosome in isolates of different STs (CG92 and novel CG786), whereas\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-24-like<\/jats:sub>\n            was carried on plasmids in CRAB isolates of limited STs (CG92). No class A or B carbapenemase genes were identified. Multiple aminoglycoside resistance genes coexisted in CRAB. Tn\n            <jats:italic>6180<\/jats:italic>\n            -borne\n            <jats:italic>armA<\/jats:italic>\n            was found in 74 (90.2%) CRAB isolates, and 58 (70.7%) isolates had Tn\n            <jats:italic>6179<\/jats:italic>\n            upstream, constituting AbGRI3.\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM<\/jats:sub>\n            was present in 38 (46.3%) of the CRAB isolates tested, with 35 (92.1%) isolates containing\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM<\/jats:sub>\n            in AbGRI2-type structures, and 61% of\n            <jats:italic>ampC<\/jats:italic>\n            genes had IS\n            <jats:italic>Aba1<\/jats:italic>\n            upstream. We conclude that the dissemination and spread of a few dominant lineages of CRAB containing various resistance island structures occurred in Taiwan.\n          <\/jats:p>","DOI":"10.1128\/aac.01215-17","type":"journal-article","created":{"date-parts":[[2018,1,5]],"date-time":"2018-01-05T20:31:03Z","timestamp":1515184263000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Molecular Epidemiology of Emerging\n            <i>bla<\/i>\n            <sub>OXA-23-Like<\/sub>\n            - and\n            <i>bla<\/i>\n            <sub>OXA-24-Like<\/sub>\n            -Carrying Acinetobacter baumannii in Taiwan"],"prefix":"10.1128","volume":"62","author":[{"given":"Shu-Chen","family":"Kuo","sequence":"first","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Cheng","family":"Huang","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tzu-Wen","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui-Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jui-Fen","family":"Lai","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Te-Li","family":"Chen","sequence":"additional","affiliation":[{"name":"Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsai-Ling","family":"Lauderdale","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, 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