{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:04:59Z","timestamp":1791273899788,"version":"4.1.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2006,7,1]],"date-time":"2006-07-01T00:00:00Z","timestamp":1151712000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            \u03b2-Lactamases are the main cause of bacterial resistance to penicillins and cephalosporins. Class A \u03b2-lactamases, the largest group of \u03b2-lactamases, have been found in many bacterial strains, including mycobacteria, for which no \u03b2-lactamase structure has been previously reported. The crystal structure of the class A \u03b2-lactamase from\n            <jats:italic>Mycobacterium fortuitum<\/jats:italic>\n            (MFO) has been solved at 2.13-A\u030a resolution. The enzyme is a chromosomally encoded broad-spectrum \u03b2-lactamase with low specific activity on cefotaxime. Specific features of the active site of the class A \u03b2-lactamase from\n            <jats:italic>M. fortuitum<\/jats:italic>\n            are consistent with its specificity profile. Arg278 and Ser237 favor cephalosporinase activity and could explain its broad substrate activity. The MFO active site presents similarities with the CTX-M type extended-spectrum \u03b2-lactamases but lacks a specific feature of these enzymes, the VNYN motif (residues 103 to 106), which confers on CTX-M-type extended-spectrum \u03b2-lactamases a more efficient cefotaximase activity.\n          <\/jats:p>","DOI":"10.1128\/aac.01226-05","type":"journal-article","created":{"date-parts":[[2006,6,26]],"date-time":"2006-06-26T18:26:28Z","timestamp":1151346388000},"page":"2516-2521","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Crystal Structure of the\n            <i>Mycobacterium fortuitum<\/i>\n            Class A \u03b2-Lactamase: Structural Basis for Broad Substrate Specificity"],"prefix":"10.1128","volume":"50","author":[{"given":"Eric","family":"Sauvage","sequence":"first","affiliation":[{"name":"Centre d'Inge\u0301nierie des Prote\u0301ines, Universite\u0301 de Lie\u0300ge, Institut de Physique B5 and Institut de Chimie B6, Sart Tilman, B-4000 Lie\u0300ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eveline","family":"Fonze\u0301","sequence":"additional","affiliation":[{"name":"Centre d'Inge\u0301nierie des Prote\u0301ines, Universite\u0301 de Lie\u0300ge, Institut de Physique B5 and Institut de Chimie B6, Sart Tilman, B-4000 Lie\u0300ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"Quinting","sequence":"additional","affiliation":[{"name":"Centre d'Inge\u0301nierie des Prote\u0301ines, Universite\u0301 de Lie\u0300ge, Institut de Physique B5 and Institut de Chimie B6, Sart Tilman, B-4000 Lie\u0300ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moreno","family":"Galleni","sequence":"additional","affiliation":[{"name":"Centre d'Inge\u0301nierie des Prote\u0301ines, Universite\u0301 de Lie\u0300ge, Institut de Physique B5 and Institut de Chimie B6, Sart Tilman, B-4000 Lie\u0300ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marie","family":"Fre\u0300re","sequence":"additional","affiliation":[{"name":"Centre d'Inge\u0301nierie des Prote\u0301ines, Universite\u0301 de Lie\u0300ge, Institut de Physique B5 and Institut de Chimie B6, Sart Tilman, B-4000 Lie\u0300ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulette","family":"Charlier","sequence":"additional","affiliation":[{"name":"Centre d'Inge\u0301nierie des Prote\u0301ines, Universite\u0301 de Lie\u0300ge, Institut de Physique B5 and Institut de Chimie B6, Sart Tilman, B-4000 Lie\u0300ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2710729"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.1-14.2004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.46.29144"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444994003112"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.02.010"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2450911"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-461X(1999)73:2<161::AID-QUA10>3.0.CO;2-E"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.36.5.1068"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi015789k"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2070429"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.7.1608"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb13331.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.9.5438"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.5.1182"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340060310"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9928041"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi0342822"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.5.1134"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2710399"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1994.tb07194.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.1.221"},{"key":"e_1_3_2_24_2","first-page":"50","volume":"5","year":"1991","unstructured":"Leslie, A. 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