{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T09:57:22Z","timestamp":1786960642414,"version":"build-2736575974"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T00:00:00Z","timestamp":1738281600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T00:00:00Z","timestamp":1738281600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82202583"],"award-info":[{"award-number":["82202583"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82272374"],"award-info":[{"award-number":["82272374"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2025,1,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              The global rise of carbapenem-resistant\n              <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n              , including strains producing\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              carbapenemase (KPC) types, poses a significant public health challenge due to their resistance to critical antibiotics. Treatment options for infections caused by KPC-producing\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              (KPC-KP) are increasingly limited, particularly as these strains develop resistance to last-line antibiotics such as ceftazidime\/avibactam and colistin. This study investigates the evolution of antibiotic resistance and persistence in a series of clonally related ST11 KPC-KP strains isolated from a single patient undergoing extended antimicrobial treatment. The patient, a 47-year-old male with a history of kidney transplantation, developed multiple KPC-KP lung infections during his hospital stay. Resistance to colistin and ceftazidime\/avibactam emerged during treatment with these antibiotics. Key resistance mechanisms identified included the integration of IS\n              <jats:italic>Kpn26<\/jats:italic>\n              into\n              <jats:italic>mgrB<\/jats:italic>\n              gene, leading to\n              <jats:italic>mgrB<\/jats:italic>\n              inactivation and colistin resistance, and the emergence of novel\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>KPC-2<\/jats:sub>\n              variants (\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>KPC-71<\/jats:sub>\n              and\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>KPC-179<\/jats:sub>\n              ) that confer resistance to ceftazidime\/avibactam. Despite the development of colistin resistance in a ceftazidime\/avibactam-resistant KPC-KP strain following combination therapy, the patient\u2019s clinical condition significantly improved. Phenotypic assays showed that\n              <jats:italic>mgrB<\/jats:italic>\n              disruption in KPC-KP resulted in increased biofilm formation and higher susceptibility to phagocytosis. In mouse models, KPC-KP strains with\n              <jats:italic>mgrB<\/jats:italic>\n              disruption showed reduced virulence, increased lung colonization and persistence, and a lower inflammatory response, suggesting that\n              <jats:italic>mgrB<\/jats:italic>\n              disruption facilitates the transition from acute infection to colonization. This study highlights the complex interplay between antibiotic resistance and bacterial fitness, offering insights into why some patients experience clinical improvement despite severe drug resistance and incomplete bacterial clearance.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.01235-24","type":"journal-article","created":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T14:01:15Z","timestamp":1733839275000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Adaptive evolution of extensive drug resistance and persistence in epidemic ST11 KPC-producing\n            <i>Klebsiella pneumoniae<\/i>\n            during antimicrobial chemotherapy"],"prefix":"10.1128","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3077-0578","authenticated-orcid":false,"given":"Tinghua","family":"Li","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoxiu","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Shanghai YangZhi Rehabilitation Hospital (Shanghai Sunshine Rehabilitation Center), School of Medicine, Tongji University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haihui","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8571-2909","authenticated-orcid":false,"given":"Min","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1034-0705","authenticated-orcid":true,"given":"Zhen","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(22)00009-3"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2024.101083"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2023.106873"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(22)01011-X"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00008-23"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00447-22"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00064-16"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2017.08.004"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1386478"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1277320"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000788"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3047"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1303641110"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02149-0"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00018-20"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04998-14"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201607336"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.03595-21"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa345"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz305"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkad326"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03110-14"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01675-19"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41426-018-0048-7"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0825-4"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00016-24"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2004.05.008"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00859-21"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.841748"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00850-19"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2009.09.004"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07830"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1159912"}],"container-title":["Antimicrobial 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