{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T04:23:51Z","timestamp":1790137431650,"version":"4.0.1"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Antifungal susceptibility testing is an essential tool for guiding therapy, although EUCAST and CLSI reference methods are often available only in specialized centers. We studied the performance of an agar-based screening method for the detection of azole resistance in\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            cultures. The VIPcheck consists of four wells containing voriconazole, itraconazole, posaconazole, or a growth control. Ninety-six\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            isolates were used. Thirty-three isolates harbored a known resistance mechanism: TR\n            <jats:sub>34<\/jats:sub>\n            \/L98H (11 isolates), TR\n            <jats:sub>46<\/jats:sub>\n            \/Y121F\/T289A (6 isolates), TR\n            <jats:sub>53<\/jats:sub>\n            (2 isolates), and 14 isolates with other\n            <jats:italic>cyp51A<\/jats:italic>\n            gene point mutations. Eighteen resistant isolates had no\n            <jats:italic>cyp51A<\/jats:italic>\n            -mediated azole resistance. Forty-five isolates had a wild-type (WT) azole phenotype. Four technicians and two inexperienced interns, blinded to the genotype\/phenotype, read the plates visually after 24 h and 48 h and documented minimal growth, uninhibited growth, and no growth. The performance was compared to the EUCAST method. After 24 h of incubation, the mean sensitivity and specificity were 0.54 and 1.00, respectively, with uninhibited growth as the threshold. After 48 h of incubation, the performance mean sensitivity and specificity were 0.98 and 0.93, respectively, with minimal growth. The performance was not affected by observer experience in mycology. The interclass correlation coefficient was 0.87 after 24 h and 0.85 after 48 h. VIPcheck enabled the selection of azole-resistant\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            colonies, with a mean sensitivity and specificity of 0.98 and 0.93, respectively. Uninhibited growth on any azole-containing well after 24 h and minimal growth after 48 h were indicative of resistance. These results indicate that the VIPcheck is an easy-to-use tool for azole resistance screening and the selection of colonies that require MIC testing.\n          <\/jats:p>","DOI":"10.1128\/aac.01250-17","type":"journal-article","created":{"date-parts":[[2017,9,19]],"date-time":"2017-09-19T00:40:23Z","timestamp":1505781623000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["Single-Center Evaluation of an Agar-Based Screening for Azole Resistance in Aspergillus fumigatus by Using VIPcheck"],"prefix":"10.1128","volume":"61","author":[{"given":"J. B.","family":"Buil","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands"},{"name":"Center of Expertise in Mycology Radboudumc\/CWZ, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. A. L.","family":"van der Lee","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands"},{"name":"Center of Expertise in Mycology Radboudumc\/CWZ, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. J. M. M.","family":"Rijs","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands"},{"name":"Center of Expertise in Mycology Radboudumc\/CWZ, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Zoll","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands"},{"name":"Center of Expertise in Mycology Radboudumc\/CWZ, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. A. M. F.","family":"Hovestadt","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. J. G.","family":"Melchers","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands"},{"name":"Center of Expertise in Mycology Radboudumc\/CWZ, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. E.","family":"Verweij","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands"},{"name":"Center of Expertise in Mycology Radboudumc\/CWZ, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003633"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1710.110226"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0050219"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit320"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku566"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(09)70265-8"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ885"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2015.0460"},{"key":"e_1_3_3_10_2","unstructured":"de Greeff\nSC MoutonJW. 2017. NethMap 2017: consumption of antimicrobial agents and antimicrobial resistance among medically important bacteria in the Netherlands\/MARAN 2017: monitoring of antimicrobial resistance and antibiotic usage in animals in the Netherlands in 2016. RIVM report 2017-0056 149\u2013154."},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmni.2015.04.005"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02855-14"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12047"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201510-2104LE"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2015.08.001"},{"key":"e_1_3_3_16_2","volume-title":"EUCAST definitive document E.Def 9.3. 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Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; approved standard, 2nd ed. CLSI document M38-A2. Clinical and Laboratory Standards Institute, Wayne, PA.","edition":"2"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2009.01781.x"},{"key":"e_1_3_3_19_2","volume-title":"26th Eur Cong Clin Microbiol Infect Dis","author":"Buil JB","year":"2016","unstructured":"Buil JB van der LeeHA RijsAJMM ZollJ HovestadtJAMF MelchersWJG VerweijPE. 2016. Agar based screening of azole resistance in Aspergillus fumigatus, abstr P1618. 26th Eur Cong Clin Microbiol Infect Dis, Amsterdam, The Netherlands, 9 to 12 April 2016."},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2006.08.017"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.2.577-581.2003"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.3.1120-1124.2003"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.5.1719-1726.2003"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.3109\/13693780902717018"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.7.2747-2750.2004"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01092-06"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05088-11"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00156-08"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01141-13"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2009.09.002"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclinepi.2004.12.009"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01250-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01250-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T20:48:01Z","timestamp":1645476481000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01250-17"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":30,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1128\/AAC.01250-17"],"URL":"https:\/\/doi.org\/10.1128\/aac.01250-17","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12]]},"assertion":[{"value":"2017-06-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-09-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-11-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01250-17"}}