{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T01:28:43Z","timestamp":1789954123092,"version":"4.0.1"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The diversity of OXA-48-like carbapenemases is continually expanding. In this study, we describe the dissemination and characteristics of a novel carbapenem-hydrolyzing class D \u03b2-lactamase (CHDL) named OXA-436. In total, six OXA-436-producing\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            isolates, including\n            <jats:named-content content-type=\"genus-species\">Enterobacter asburiae<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 3),\n            <jats:named-content content-type=\"genus-species\">Citrobacter freundii<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 2), and\n            <jats:named-content content-type=\"genus-species\">Klebsiella pneumoniae<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 1), were identified in four patients in the period between September 2013 and April 2015. All three species of OXA-436-producing\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            were found in one patient. The amino acid sequence of OXA-436 showed 90.4 to 92.8% identity to the amino acid sequences of other acquired OXA-48-like variants. Expression of OXA-436 in\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            and kinetic analysis of purified OXA-436 revealed an activity profile similar to that of OXA-48 and OXA-181, with activity against penicillins, including temocillin; limited or no activity against extended-spectrum cephalosporins; and activity against carbapenems. The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-436<\/jats:sub>\n            gene was located on a conjugative \u223c314-kb IncHI2\/IncHI2A plasmid belonging to plasmid multilocus sequence typing sequence type 1 in a region surrounded by chromosomal genes previously identified to be adjacent to\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA<\/jats:sub>\n            genes in\n            <jats:named-content content-type=\"genus-species\">Shewanella<\/jats:named-content>\n            spp. In conclusion, OXA-436 is a novel CHDL with functional properties similar to those of OXA-48-like CHDLs. The described geographical spread among different\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            and the plasmid location of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-436<\/jats:sub>\n            illustrate its potential for further dissemination.\n          <\/jats:p>","DOI":"10.1128\/aac.01260-17","type":"journal-article","created":{"date-parts":[[2017,10,24]],"date-time":"2017-10-24T00:40:25Z","timestamp":1508805625000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Dissemination and Characteristics of a Novel Plasmid-Encoded Carbapenem-Hydrolyzing Class D \u03b2-Lactamase, OXA-436, Found in Isolates from Four Patients at Six Different Hospitals in Denmark"],"prefix":"10.1128","volume":"62","author":[{"given":"\u00d8rjan","family":"Samuelsen","sequence":"first","affiliation":[{"name":"Norwegian National Advisory Unit on Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, Troms\u00f8, Norway"},{"name":"Microbial Pharmacology and Population Biology Research Group, Department of Pharmacy, UiT, The Arctic University of Norway, Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bettina","family":"Aasn\u00e6s","sequence":"additional","affiliation":[{"name":"Norwegian National Advisory Unit on Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Hasman","sequence":"additional","affiliation":[{"name":"Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bjarte Aarmo","family":"Lund","sequence":"additional","affiliation":[{"name":"The Norwegian Structural Biology Centre (NorStruct), Department of Chemistry, UiT, The Arctic University of Norway, Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna-Kirsti S.","family":"Leiros","sequence":"additional","affiliation":[{"name":"The Norwegian Structural Biology Centre (NorStruct), Department of Chemistry, UiT, The Arctic University of Norway, Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berit","family":"Lilje","sequence":"additional","affiliation":[{"name":"Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessin","family":"Janice","sequence":"additional","affiliation":[{"name":"Norwegian National Advisory Unit on Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, Troms\u00f8, Norway"},{"name":"Research Group on Host-Microbe Interactions, Department of Medical Biology, UiT, The Arctic University of Norway, Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lotte","family":"Jakobsen","sequence":"additional","affiliation":[{"name":"Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pia","family":"Littauer","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Hvidovre University Hospital, Hvidovre, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lillian M.","family":"S\u00f8es","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Slagelse Hospital, Slagelse, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara J.","family":"Holzknecht","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Slagelse Hospital, Slagelse, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leif P.","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Stegger","sequence":"additional","affiliation":[{"name":"Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Copenhagen, Denmark"},{"name":"Pathogen Genomics Division, Translational Genomics Research Institute (TGen), Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paal S.","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Copenhagen, Denmark"},{"name":"Pathogen Genomics Division, Translational Genomics Research Institute (TGen), Flagstaff, Arizona, USA"},{"name":"Veterinary Disease Biology, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anette M.","family":"Hammerum","sequence":"additional","affiliation":[{"name":"Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00117-13"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01512-08"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.15-22.2004"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12719"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02510-13"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05058-14"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES2013.18.31.20549"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0123063"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048280"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.348-351.2004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00681-11"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku524"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00022-11"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12142"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00481-11"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks261"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-015-2375-0"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt367"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02121-14"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02761-13"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq101"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2004.06.006"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00970-08"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2016.04.001"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0135044"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0902000106"},{"key":"e_1_3_2_28_2","volume-title":"abstr P0233","author":"Dabos MJ","year":"2017","unstructured":"Dabos MJ, Potron A, Dortet A, Iorga L, Bonnin B, Naas RT. 2017. Genetic and biochemical characterization of OXA-535, a novel OXA-48-like enzyme progenitor of OXA-436 from Shewanella bicestrii, abstr P0233. Abstr 27th Eur Congr Clin Microbiol Infect Dis, Vienna, Austria."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2009.04.010"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv181"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02522-13"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0134(19981115)33:3<358::AID-PROT5>3.0.CO;2-0"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt058"},{"key":"e_1_3_2_34_2","volume-title":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, tenth edition: approved standard M07-A10","author":"Clinical and Laboratory Standards Institute","year":"2015","unstructured":"Clinical and Laboratory Standards Institute. 2015. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, tenth edition: approved standard M07-A10. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.071340-0"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2013.0085"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.12.4793-4799.2004"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp324"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.11.2478"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06094-11"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02412-14"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003018"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu033"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbx108"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku316"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti054"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-13-38"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02227-12"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2005.01.016"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.5.1973-1980.2005"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01260-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01260-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:07:36Z","timestamp":1645477656000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01260-17"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1]]},"references-count":51,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1128\/AAC.01260-17"],"URL":"https:\/\/doi.org\/10.1128\/aac.01260-17","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1]]},"assertion":[{"value":"2017-06-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-12-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01260-17"}}