{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:33:20Z","timestamp":1790393600869,"version":"4.1.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>We prepared monoclonal antibodies against<jats:italic>N<\/jats:italic>-(\u03b3-maleimidobutyryloxy)succinimide-conjugated vancomycin (VM). The monoclonal antibody was specific for conjugated or free VM. The monoclonal antibody enabled us to develop an immunocytochemical method for detecting the uptake of VM in the rat kidney and liver. Three hours after a single intravenous (i.v.) injection of VM at the therapeutic dose, the immunocytochemistry revealed that VM accumulated in large amounts in both the S1 and S2 segments and in much smaller amounts in the S3 segment of the proximal tubules as well as in the distal tubules and collecting ducts. The drug was detected in the cytoplasm, cytoplasmic irregular granules, nuclei, and microvilli of the proximal tubule cells. The distal tubules and collecting ducts contained scattered swollen cells in which both the nuclei and cytoplasm were heavily immunostained. Twenty-four hours after injection, most of the swollen cells returned back to normal size and had somewhat decreased immunostaining. Also, significant amounts of VM remained accumulated for as long as 8 days postadministration. In the liver, similar drug accumulation was observed in the Kupffer cells and the endothelial cells of the hepatic sinusoids but not in the hepatocytes, suggesting that vancomycin cannot be eliminated via the liver. Immunoelectron microscopic studies demonstrated that in the collecting ducts, uptake of VM occurred exclusively in the lysosomes and cytoplasm of the principal cells and scarcely in the intercalated cells. Furthermore, double fluorescence staining using rats simultaneously administered with VM and gentamicin strongly suggests that both drugs colocalized in lysosomes in the proximal tubule cells of kidneys.<\/jats:p>","DOI":"10.1128\/aac.01267-12","type":"journal-article","created":{"date-parts":[[2012,9,5]],"date-time":"2012-09-05T06:53:01Z","timestamp":1346827981000},"page":"5883-5891","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Immunocytochemistry for Vancomycin Using a Monoclonal Antibody That Reveals Accumulation of the Drug in Rat Kidney and Liver"],"prefix":"10.1128","volume":"56","author":[{"given":"Kunio","family":"Fujiwara","sequence":"first","affiliation":[{"name":"Department of Applied Life Science, Faculty of Biotechnology and Life Science, Sojo University, Ikeda, Kumamoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohei","family":"Yoshizaki","sequence":"additional","affiliation":[{"name":"Department of Applied Life Science, Faculty of Biotechnology and Life Science, Sojo University, Ikeda, Kumamoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Applied Life Science, Faculty of Biotechnology and Life Science, Sojo University, Ikeda, Kumamoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsubasa","family":"Miyazaki","sequence":"additional","affiliation":[{"name":"Department of Applied Life Science, Faculty of Biotechnology and Life Science, Sojo University, Ikeda, Kumamoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Saita","sequence":"additional","affiliation":[{"name":"Department of Applied Life Science, Faculty of Biotechnology and Life Science, Sojo University, Ikeda, Kumamoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuichi","family":"Nagata","sequence":"additional","affiliation":[{"name":"Sakura-machi Pharmacy, Ebisu-machi, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.11.2173"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.36.10.2204"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05609-11"},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0165-0270(98)00066-1","article-title":"Reduction of background autofluorescence in brain sections following immersion in sodium borohydride","volume":"83","author":"Clancy B","year":"1998","unstructured":"ClancyB CaullerLJ. 1998. Reduction of background autofluorescence in brain sections following immersion in sodium borohydride. J. Neurosci. Methods 83:97\u2013102.","journal-title":"J. Neurosci. Methods"},{"key":"e_1_3_3_6_2","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1177\/35.11.3309046","article-title":"First visualization of dopaminenergic neurons with a monoclonal antibody to dopamine: a light and electron microscopic study","volume":"35","author":"Decavel C","year":"1987","unstructured":"DecavelC LescaudronL MonsN CalasA. 1987. First visualization of dopaminenergic neurons with a monoclonal antibody to dopamine: a light and electron microscopic study. J. Histochem. Cytochem. 35:1245\u20131251.","journal-title":"J. Histochem. Cytochem."},{"issue":"3","key":"e_1_3_3_7_2","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1038\/nrd2438","article-title":"Carrier-mediated cellular uptake of pharmaceutical drugs: an exception or the rule?","volume":"7","author":"Dobson PD","year":"2008","unstructured":"DobsonPD KellDB. 2008. Carrier-mediated cellular uptake of pharmaceutical drugs: an exception or the rule? Nat. Rev. Drug Discov. 7(3):205\u2013220.","journal-title":"Nat. Rev. Drug Discov."},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1002\/aja.1001530312","article-title":"Somatostatin-containing neurons in the rat brain: widespread distribution revealed by immunocytochemistry after pretreatment with pronase","volume":"153","author":"Finley JC","year":"1978","unstructured":"FinleyJC GrossmanGH DimeoP PetruszP. 1978. Somatostatin-containing neurons in the rat brain: widespread distribution revealed by immunocytochemistry after pretreatment with pronase. Am. J. Anat. 153:483\u2013488.","journal-title":"Am. J. Anat."},{"key":"e_1_3_3_9_2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0022-1759(81)90213-1","article-title":"Novel preparation method of immunogen for hydrophobic hapten, enzyme immunoassay for daunomycin and adriamycin","volume":"45","author":"Fujiwara K","year":"1981","unstructured":"FujiwaraK YasunoM KitagawaT. 1981. Novel preparation method of immunogen for hydrophobic hapten, enzyme immunoassay for daunomycin and adriamycin. J. Immunol. Methods 45:195\u2013203.","journal-title":"J. Immunol. Methods"},{"key":"e_1_3_3_10_2","first-page":"1487","article-title":"Enzyme immunoassay for the quantification of mitomycin C using \u03b2-galactosidase as a label","volume":"42","author":"Fujiwara K","year":"1982","unstructured":"FujiwaraK SaikusaH YasunoM KitagawaT. 1982. Enzyme immunoassay for the quantification of mitomycin C using \u03b2-galactosidase as a label. Cancer Res. 42:1487\u20131491.","journal-title":"Cancer Res."},{"key":"e_1_3_3_11_2","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1007\/978-1-4613-9558-4_6","volume-title":"Advances in immunity and cancer therapy","author":"Fujiwara K","year":"1986","unstructured":"FujiwaraK KitagawaT. 1986 Specificity and use of antisera produced against anticancer drugs, p 251\u2013292. In RayPK (ed), Advances in immunity and cancer therapy, vol 2. Springer-Verlag, New York, NY."},{"key":"e_1_3_3_12_2","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1007\/BF01464327","article-title":"Monoclonal antibody against the glutaraldehyde-conjugated polyamine, spermine","volume":"104","author":"Fujiwara K","year":"1995","unstructured":"FujiwaraK MasuyamaY. 1995. Monoclonal antibody against the glutaraldehyde-conjugated polyamine, spermine. Histochem. Cell Biol. 104:309\u2013316.","journal-title":"Histochem. Cell Biol."},{"key":"e_1_3_3_13_2","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1007\/BF02473308","article-title":"Immunocytochemical localization of polyamines in the gastrointestinal tracts of rats and mice","volume":"106","author":"Fujiwara K","year":"1996","unstructured":"FujiwaraK MasuyamaY KitagawaT. 1996. Immunocytochemical localization of polyamines in the gastrointestinal tracts of rats and mice. Histochem. Cell Biol. 106:465\u2013471.","journal-title":"Histochem. Cell Biol."},{"key":"e_1_3_3_14_2","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1177\/002215549804601112","article-title":"Immunoelectron microscopic study for polyamines","volume":"46","author":"Fujiwara K","year":"1998","unstructured":"FujiwaraK BaiB KitagawaT TsuruD. 1998. Immunoelectron microscopic study for polyamines. J. Histochem. Cytochem. 46:1321\u20131328.","journal-title":"J. Histochem. Cytochem."},{"key":"e_1_3_3_15_2","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1177\/002215549904700807","article-title":"Immunocytochemical localization of histamine in enterochromaffin-like (ECL) cells in rat oxyntic mucosa: a transmission electron microscopy study using monoclonal antibodies and conventional glutaraldehyde-based fixation","volume":"47","author":"Fujiwara K","year":"1999","unstructured":"FujiwaraK BaiG TamuraC TsuruD. 1999. Immunocytochemical localization of histamine in enterochromaffin-like (ECL) cells in rat oxyntic mucosa: a transmission electron microscopy study using monoclonal antibodies and conventional glutaraldehyde-based fixation. J. Histochem. Cytochem. 47:1031\u20131038.","journal-title":"J. Histochem. Cytochem."},{"key":"e_1_3_3_16_2","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1007\/s004180100280","article-title":"A monoclonal antibody against the glutaraldehyde-conjugated polyamine, putrescine: application to immunocytochemistry","volume":"115","author":"Fujiwara K","year":"2001","unstructured":"FujiwaraK TanabeT YabuuchiM UeokaR TsuruD. 2001. A monoclonal antibody against the glutaraldehyde-conjugated polyamine, putrescine: application to immunocytochemistry. Histochem. Cell Biol. 115:471\u2013477.","journal-title":"Histochem. Cell Biol."},{"key":"e_1_3_3_17_2","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1369\/jhc.4A6459.2005","article-title":"Immunocytochemistry for drugs containing an aliphatic amino group in the molecule, anticancer antibiotic daunomycin as a model","volume":"53","author":"Fujiwara K","year":"2005","unstructured":"FujiwaraK TakatsuH TsukamotoK. 2005. Immunocytochemistry for drugs containing an aliphatic amino group in the molecule, anticancer antibiotic daunomycin as a model. J. Histochem. Cytochem. 53:467\u2013474.","journal-title":"J. Histochem. Cytochem."},{"key":"e_1_3_3_18_2","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s00418-006-0216-z","article-title":"Distribution of anticancer antibiotic daunomycin in the rat heart and kidney revealed by immunocytochemistry using monoclonal antibodies","volume":"127","author":"Fujiwara K","year":"2007","unstructured":"FujiwaraK ShinM HougaardDM LarssonL-I. 2007. Distribution of anticancer antibiotic daunomycin in the rat heart and kidney revealed by immunocytochemistry using monoclonal antibodies. Histochem. Cell Biol. 127:69\u201377.","journal-title":"Histochem. Cell Biol."},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01627-08"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01031-10"},{"key":"e_1_3_3_21_2","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s00418-010-0768-9","article-title":"Distribution study of peplomycin in rat kidney revealed by immunocytochemistry using monoclonal antibody","volume":"135","author":"Fujiwara K","year":"2011","unstructured":"FujiwaraK ShinM HougaardDH SaitaT. 2011. Distribution study of peplomycin in rat kidney revealed by immunocytochemistry using monoclonal antibody. Histochem. Cell Biol. 135:93\u2013101.","journal-title":"Histochem. Cell Biol."},{"key":"e_1_3_3_22_2","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1007\/s10735-012-9406-2","article-title":"An in vivo role of Mrp2 in the rat hepatocytes by immunocytochemistry for amoxicillin using the transporter-deficient EHBR","volume":"43","author":"Fujiwara K","year":"2012","unstructured":"FujiwaraK ShinM YoshizakiY MiyazakiT SaitaT. 2012. An in vivo role of Mrp2 in the rat hepatocytes by immunocytochemistry for amoxicillin using the transporter-deficient EHBR. J. Mol. Histol. 43:371\u2013378.","journal-title":"J. Mol. Histol."},{"key":"e_1_3_3_23_2","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1146\/annurev.bi.62.070193.002125","article-title":"Biochemistry of multidrug resistance mediated by the multi-drug transporter","volume":"62","author":"Gottesman MM","year":"1993","unstructured":"GottesmanMM PastanI. 1993. Biochemistry of multidrug resistance mediated by the multi-drug transporter. Annu. Rev. Biochem. 62:385\u2013427.","journal-title":"Annu. Rev. Biochem."},{"key":"e_1_3_3_24_2","first-page":"379","article-title":"Vancomycin nephrotoxicity: myths and facts","volume":"69","author":"Gupta A","year":"2011","unstructured":"GuptaA BiyaniM KhairaA. 2011. Vancomycin nephrotoxicity: myths and facts. Neth. J. Med. 69:379\u2013383.","journal-title":"Neth. J. Med."},{"key":"e_1_3_3_25_2","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s00418-001-0363-1","article-title":"Immunofluorescence technique for 100-nm-thick semithin sections of Epon-embedded tissues","volume":"117","author":"Haraguchi CM","year":"2002","unstructured":"HaraguchiCM YokotaS. 2002. Immunofluorescence technique for 100-nm-thick semithin sections of Epon-embedded tissues. Histochem. Cell Biol. 117:81\u201385.","journal-title":"Histochem. Cell Biol."},{"key":"e_1_3_3_26_2","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1177\/36.5.3282006","article-title":"The effects of fixation time and enzymatic digestion on the immunohistochemical demonstration of bromodeoxyuridine in formalin-fixed, paraffin-embedded tissue","volume":"36","author":"Hayashi A","year":"1988","unstructured":"HayashiA KoikeM MatsunamiM HoshinoT. 1988. The effects of fixation time and enzymatic digestion on the immunohistochemical demonstration of bromodeoxyuridine in formalin-fixed, paraffin-embedded tissue. J. Histochem. Cytochem. 36:511\u2013514.","journal-title":"J. Histochem. Cytochem."},{"key":"e_1_3_3_27_2","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1177\/36.1.2891743","article-title":"Characterization of antisera to glutamate and aspartate","volume":"36","author":"Hepler JR","year":"1988","unstructured":"HeplerJR. 1988. Characterization of antisera to glutamate and aspartate. J. Histochem. Cytochem. 36:13\u201322.","journal-title":"J. Histochem. Cytochem."},{"key":"e_1_3_3_28_2","doi-asserted-by":"crossref","first-page":"419","DOI":"10.2133\/dmpk.22.419","article-title":"Decreased renal accumulation and toxicity of a new VCM formulation in rats with chronic renal failure","volume":"22","author":"Hodoshima N","year":"2007","unstructured":"HodoshimaN MasudaS InuiK. 2007. Decreased renal accumulation and toxicity of a new VCM formulation in rats with chronic renal failure. Drug Metab. Pharmacokinet. 22:419\u2013427.","journal-title":"Drug Metab. Pharmacokinet."},{"key":"e_1_3_3_29_2","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1046\/j.1523-1755.2000.00251.x","article-title":"Cellular and molecular aspects of drug transport in the kidney","volume":"58","author":"Inui K","year":"2000","unstructured":"InuiK MasudaS SaitoH. 2000. Cellular and molecular aspects of drug transport in the kidney. Kidney Int. 58:944\u2013958.","journal-title":"Kidney Int."},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1086\/491709"},{"key":"e_1_3_3_31_2","first-page":"F1","article-title":"Structural-functional relationship along the distal nephron","volume":"250","author":"Madsen KM","year":"1986","unstructured":"MadsenKM TissherCC. 1986. Structural-functional relationship along the distal nephron. Am. J. Physiol. 250:F1\u2013F15.","journal-title":"Am. J. Physiol."},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-198611040-00001"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1086\/491708"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1996.tb03920.x"},{"key":"e_1_3_3_35_2","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1177\/35.3.3819375","article-title":"An improved immunocytochemical method for subcellular localization of serotonin in rat enterochromaffin cells","volume":"35","author":"Nilsson O","year":"1987","unstructured":"NilssonO DahlstromA GeffardM AhlmanH EricsonLE. 1987. An improved immunocytochemical method for subcellular localization of serotonin in rat enterochromaffin cells. J. Histochem. Cytochem. 35:319\u2013326.","journal-title":"J. Histochem. Cytochem."},{"key":"e_1_3_3_36_2","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1007\/s00418-006-0267-1","article-title":"Improved immunocytochemical detection of daunomycin","volume":"127","author":"Ohara K","year":"2007","unstructured":"OharaK ShinM LarssonL-I FujiwaraK. 2007. Improved immunocytochemical detection of daunomycin. Histochem. Cell Biol. 127:603\u2013608.","journal-title":"Histochem. Cell Biol."},{"key":"e_1_3_3_37_2","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/0006-8993(95)01280-X","article-title":"Colocalization of GABA and glycine in the rat dorsal column nuclei","volume":"706","author":"Popratiloff A","year":"1996","unstructured":"PopratiloffA ValtschanoffJG RustioniA WeinbergRJ. 1996. Colocalization of GABA and glycine in the rat dorsal column nuclei. Brain Res. 706:308\u2013312.","journal-title":"Brain Res."},{"key":"e_1_3_3_38_2","doi-asserted-by":"crossref","first-page":"S35-S-39","DOI":"10.1086\/491712","article-title":"The pharmacokinetic and pharmacodynamic properties of vancomycin","volume":"42","author":"Rybak MR","year":"2006","unstructured":"RybakMR. 2006. The pharmacokinetic and pharmacodynamic properties of vancomycin. Clin. Infect. Dis. 42:S35-S-39.","journal-title":"Clin. Infect. Dis."},{"issue":"8","key":"e_1_3_3_39_2","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1681\/ASN.V881213","article-title":"Expression of the conjugate export pump encoded by the mrp2 gene in the apical membrane of kidney proximal tubules","volume":"8","author":"Schaub TP","year":"1997","unstructured":"SchaubTP. 1997. Expression of the conjugate export pump encoded by the mrp2 gene in the apical membrane of kidney proximal tubules. J. Am. Soc. Nephrol. 8(8):1213\u20131221.","journal-title":"J. Am. Soc. Nephrol."},{"issue":"7","key":"e_1_3_3_40_2","doi-asserted-by":"crossref","first-page":"465","DOI":"10.3949\/ccjm.78a.10168","article-title":"Vancomycin: a 50-something-year-old antibiotic we still don't understand","volume":"78","author":"Schilling A","year":"2011","unstructured":"SchillingA NeunerE RehmSJ. 2011. Vancomycin: a 50-something-year-old antibiotic we still don't understand. Cleve Clin. J. Med. 78(7):465\u2013471.","journal-title":"Cleve Clin. J. Med."},{"key":"e_1_3_3_41_2","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1007\/s00418-007-0285-7","article-title":"Polyamines in spermatocytes and residual bodies of rat testis","volume":"127","author":"Shin M","year":"2007","unstructured":"ShinM LarssonL-I FujiwaraK. 2007. Polyamines in spermatocytes and residual bodies of rat testis. Histochem. Cell Biol. 127:649\u2013655.","journal-title":"Histochem. Cell Biol."},{"key":"e_1_3_3_42_2","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1007\/s00418-008-0400-4","article-title":"Immunocytochemical demonstration of polyamines in nucleoli and nuclei","volume":"129","author":"Shin M","year":"2008","unstructured":"ShinM NakamutaH Oda-UedaN LarssonL-I FujiwaraK. 2008. Immunocytochemical demonstration of polyamines in nucleoli and nuclei. Histochem. Cell Biol. 129:659\u2013665.","journal-title":"Histochem. Cell Biol."},{"key":"e_1_3_3_43_2","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1007\/s00441-009-0840-8","article-title":"Daunomycin accumulation and induction of programmed cell death in rat hair follicles","volume":"337","author":"Shin M","year":"2009","unstructured":"ShinM LarssonL-I HougaardDM FujiwaraK. 2009. Daunomycin accumulation and induction of programmed cell death in rat hair follicles. Cell Tissue Res. 337:429\u2013438.","journal-title":"Cell Tissue Res."},{"key":"e_1_3_3_44_2","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1016\/S0022-3565(25)20414-1","article-title":"Mechanism of vancomycin transport in the kidney: studies in rabbit renal brush border and basolateral membrane vesicles","volume":"259","author":"Sokol PP","year":"1991","unstructured":"SokolPP. 1991. Mechanism of vancomycin transport in the kidney: studies in rabbit renal brush border and basolateral membrane vesicles. J. Pharmacol. Exp. Ther. 259:1283\u20131287.","journal-title":"J. Pharmacol. Exp. Ther."},{"issue":"4","key":"e_1_3_3_45_2","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1002\/(SICI)1096-9861(19970714)383:4<473::AID-CNE6>3.0.CO;2-#","article-title":"Glycine immunoreactivity in the lateral nucleus of the trapezoid body of the cat","volume":"383","author":"Spirou GA","year":"1997","unstructured":"SpirouGA BerrebiAS. 1997. Glycine immunoreactivity in the lateral nucleus of the trapezoid body of the cat. J. Comp. Neurol. 383(4):473\u2013488.","journal-title":"J. Comp. Neurol."},{"key":"e_1_3_3_46_2","first-page":"1926","article-title":"Tissue distribution of p-glycoprotein encoded by a multidrug-resistant gene as revealed by a monoclonal antibody, MRK 16","volume":"48","author":"Sugawara I","year":"1988","unstructured":"SugawaraI. 1988. Tissue distribution of p-glycoprotein encoded by a multidrug-resistant gene as revealed by a monoclonal antibody, MRK 16. Cancer Res. 48:1926\u20131929.","journal-title":"Cancer Res."},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5048-06.2007"},{"key":"e_1_3_3_48_2","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1016\/j.bcp.2007.12.008","article-title":"Physiological and pharmacokinetic roles of H+\/organic cation antiporters (METE\/SLC47A)","volume":"75","author":"Terada T","year":"2008","unstructured":"TeradaT InuiK. 2008. Physiological and pharmacokinetic roles of H+\/organic cation antiporters (METE\/SLC47A). Biochem. Pharmacol. 75:1689\u20131696.","journal-title":"Biochem. Pharmacol."},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.21.7735"},{"key":"e_1_3_3_50_2","first-page":"212","article-title":"Transport of gentamicin in rat proximal tubule","volume":"48","author":"Wedeen RP","year":"1983","unstructured":"WedeenRP BatumanV CheeksC MarquetE SobelH. 1983. Transport of gentamicin in rat proximal tubule. Lab. Invest. 48:212\u2013223.","journal-title":"Lab. Invest."}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01267-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01267-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T21:56:33Z","timestamp":1744062993000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01267-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11]]},"references-count":49,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.1128\/AAC.01267-12"],"URL":"https:\/\/doi.org\/10.1128\/aac.01267-12","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11]]},"assertion":[{"value":"2012-06-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-08-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-10-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}