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Here, we describe the activity of the antibiotic boromycin against<jats:italic>Toxoplasma<\/jats:italic>and<jats:italic>Cryptosporidium<\/jats:italic>. Boromycin potently inhibited intracellular proliferation of both<jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>and<jats:named-content content-type=\"genus-species\">C. parvum<\/jats:named-content>at half-maximal effective concentrations (EC<jats:sub>50<\/jats:sub>) of 2.27\u2009nM and 4.99\u2009nM, respectively.<\/jats:p>","DOI":"10.1128\/aac.01278-20","type":"journal-article","created":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T13:30:55Z","timestamp":1611149455000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Boromycin Has Potent Anti-<i>Toxoplasma<\/i>and Anti-<i>Cryptosporidium<\/i>Activity"],"prefix":"10.1128","volume":"65","author":[{"given":"Jaypee","family":"Abenoja","sequence":"first","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA"},{"name":"College of Veterinary Science and Medicine, Central Luzon State University, Science City of Mu\u00f1oz, Nueva Ecija, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexis","family":"Cotto-Rosario","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2757-754X","authenticated-orcid":true,"given":"Roberta","family":"O\u2019Connor","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16412-X"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/15.2.211"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jpids\/piu077"},{"key":"e_1_3_3_5_2","first-page":"403","article-title":"Behavioral alterations caused by parasitic infection in case of latent toxoplasma infection","volume":"250","author":"Piekarski G","year":"1981","unstructured":"Piekarski G. 1981. 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Potential risk factors for Cryptosporidium infection in dairy calves. J Am Vet Med Assoc 205:86\u201391.","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD004932.pub2"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)11401-2"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/hlca.19670500612"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008600"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00409681"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00199"},{"key":"e_1_3_3_23_2","unstructured":"Miller MB Burg RW. 1975. Boromycin as a coccidiostat. U.S. Patent 3864479."},{"key":"e_1_3_3_24_2","unstructured":"Prelog V Zaehner H Bickel H. 1973. Antibiotic A 28829. 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