{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T13:45:30Z","timestamp":1779889530142,"version":"3.53.1"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2020,12,16]],"date-time":"2020-12-16T00:00:00Z","timestamp":1608076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000265","name":"UKRI | Medical Research Council","doi-asserted-by":"publisher","award":["MR\/S004769\/1"],"award-info":[{"award-number":["MR\/S004769\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2020,12,16]]},"abstract":"<jats:p>\n                    Fluoroquinolone resistance in\n                    <jats:named-content content-type=\"genus-species\">Stenotrophomonas maltophilia<\/jats:named-content>\n                    is multifactorial, but the most significant factor is overproduction of efflux pumps, particularly SmeDEF, following mutation. Here, we report that mutations in the glycosyl transferase gene\n                    <jats:italic>smlt0622<\/jats:italic>\n                    in\n                    <jats:named-content content-type=\"genus-species\">S. maltophilia<\/jats:named-content>\n                    K279a mutant K M6 cause constitutive activation of SmeDEF production, leading to elevated levofloxacin MIC. Selection of a levofloxacin-resistant K M6 derivative, K M6 LEV\n                    <jats:sup>r<\/jats:sup>\n                    , allowed identification of a novel two-component regulatory system, Smlt2645\/6 (renamed SmaRS).\n                  <\/jats:p>","DOI":"10.1128\/aac.01284-20","type":"journal-article","created":{"date-parts":[[2020,10,29]],"date-time":"2020-10-29T10:10:31Z","timestamp":1603966231000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Novel Mechanisms of Efflux-Mediated Levofloxacin Resistance and Reduced Amikacin Susceptibility in Stenotrophomonas maltophilia"],"prefix":"10.1128","volume":"65","author":[{"given":"Punyawee","family":"Dulyayangkul","sequence":"first","affiliation":[{"name":"School of Cellular &amp; Molecular Medicine, University of Bristol, Bristol, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina","family":"Calvopi\u00f1a","sequence":"additional","affiliation":[{"name":"School of Cellular &amp; Molecular Medicine, University of Bristol, Bristol, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate J.","family":"Heesom","sequence":"additional","affiliation":[{"name":"University of Bristol Proteomics Facility, Bristol, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1454-8440","authenticated-orcid":false,"given":"Matthew B.","family":"Avison","sequence":"additional","affiliation":[{"name":"School of Cellular &amp; Molecular Medicine, University of Bristol, Bristol, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Performance standards for antimicrobial susceptibility testing; 29th informational supplement","author":"Clinical and Laboratory Standards Institute","year":"2019","unstructured":"Clinical and Laboratory Standards Institute. 2019. 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