{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T05:20:23Z","timestamp":1791523223419,"version":"4.3.4"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The development of resistance to antipseudomonal penicillins and cephalosporins mediated by the chromosomal\n                    <jats:italic>ampC<\/jats:italic>\n                    gene in\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Pseudomonas aeruginosa<\/jats:named-content>\n                    is of clinical importance. We isolated piperacillin-resistant mutants derived from\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. aeruginosa<\/jats:named-content>\n                    PAO1 and analyzed two mutants that had an insertion in\n                    <jats:italic>mpl<\/jats:italic>\n                    and\n                    <jats:italic>nuoN<\/jats:italic>\n                    . One mutant, YT1677, was resistant to piperacillin and ceftazidime and had an insertion in\n                    <jats:italic>mpl<\/jats:italic>\n                    , which encodes UDP-\n                    <jats:italic>N<\/jats:italic>\n                    -acetylmuramate:\n                    <jats:sc>l<\/jats:sc>\n                    -alanyl-\u03b3-\n                    <jats:sc>d<\/jats:sc>\n                    -glutamyl-\n                    <jats:italic>meso<\/jats:italic>\n                    -diaminopimelate ligase. The other mutant, YT7988, showed increased MICs of piperacillin, ceftazidime, cefepime, and cefoperazone, and the insertion was mapped to\n                    <jats:italic>nuoN<\/jats:italic>\n                    , which encodes NADH dehydrogenase I chain N. Complementation experiments demonstrated that these mutations resulted in higher levels of resistance to \u03b2-lactams. The expression of genes reported to be involved in \u03b2-lactam resistance was examined by real-time PCR in YT1677 and YT7988 mutants. Overexpression was observed for only\n                    <jats:italic>ampC<\/jats:italic>\n                    , and other genes were expressed normally. Deletion of the\n                    <jats:italic>ampR<\/jats:italic>\n                    gene in YT1677 and YT7988 resulted in decreased expression of\n                    <jats:italic>ampC<\/jats:italic>\n                    , indicating that the mutations in YT1677 and YT7988 affected the expression of\n                    <jats:italic>ampC<\/jats:italic>\n                    through the function of AmpR.\n                  <\/jats:p>","DOI":"10.1128\/aac.01291-13","type":"journal-article","created":{"date-parts":[[2013,9,16]],"date-time":"2013-09-16T23:45:54Z","timestamp":1379375154000},"page":"5987-5993","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["Identification of Novel Genes Responsible for Overexpression of\n                    <i>ampC<\/i>\n                    in Pseudomonas aeruginosa PAO1"],"prefix":"10.1128","volume":"57","author":[{"given":"Yuko","family":"Tsutsumi","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruyoshi","family":"Tomita","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan"},{"name":"Laboratory of Bacterial Drug Resistance, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Tanimoto","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Drug Resistance, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/35023079"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00015-06"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.306-325.2005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00040-09"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00036-08"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00816-09"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06792.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1986.tb04704.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1982.tb01263.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01388-12"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.3.1145-1149.2005"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01731.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.12.3296"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.3.583-589.2000"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.5.1780-1787.2006"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.11.4567-4575.2005"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000353"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M011186200"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01617-08"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00385-10"},{"key":"e_1_3_2_24_2","volume-title":"Performance standards for antimicrobial susceptibility testing; 22nd informational supplement","author":"Clinical and Laboratory Standards Institute","year":"2012","unstructured":"Clinical and Laboratory Standards Institute. 2012. 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