{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T23:30:23Z","timestamp":1790465423217,"version":"4.1.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"USDA NIFA","award":["2014-67005-21709"],"award-info":[{"award-number":["2014-67005-21709"]}]},{"name":"The Ohio State University Public Health Preparedness for Infectious Disease Program"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Carbapenem-resistant\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            (CRE) present an urgent threat to public health. While use of carbapenem antimicrobials is restricted for food-producing animals, other \u03b2-lactams, such as ceftiofur, are used in livestock. This use may provide selection pressure favoring the amplification of carbapenem resistance, but this relationship has not been established. Previously unreported among U.S. livestock, plasmid-mediated CRE have been reported from livestock in Europe and Asia. In this study, environmental and fecal samples were collected from a 1,500-sow, U.S. farrow-to-finish operation during 4 visits over a 5-month period in 2015. Samples were screened using selective media for the presence of CRE, and the resulting carbapenemase-producing isolates were further characterized. Of 30 environmental samples collected from a nursery room on our initial visit, 2 (7%) samples yielded 3 isolates, 2 sequence type 218 (ST 218)\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            and 1\n            <jats:named-content content-type=\"genus-species\">Proteus mirabilis<\/jats:named-content>\n            , carrying the metallo-\u03b2-lactamase gene\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-27<\/jats:sub>\n            on IncQ1 plasmids. We recovered on our third visit 15 IMP-27-bearing isolates of multiple\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            species from 11 of 24 (46%) environmental samples from 2 farrowing rooms. These isolates each also carried\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-27<\/jats:sub>\n            on IncQ1 plasmids. No CRE isolates were recovered from fecal swabs or samples in this study. As is common in U.S. swine production, piglets on this farm receive ceftiofur at birth, with males receiving a second dose at castration (\u2248day 6). This selection pressure may favor the dissemination of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-27<\/jats:sub>\n            -bearing\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            in this farrowing barn. The absence of this selection pressure in the nursery and finisher barns likely resulted in the loss of the ecological niche needed for maintenance of this carbapenem resistance gene.\n          <\/jats:p>","DOI":"10.1128\/aac.01298-16","type":"journal-article","created":{"date-parts":[[2016,12,6]],"date-time":"2016-12-06T03:00:36Z","timestamp":1480993236000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":108,"title":["Carbapenemase-Producing Enterobacteriaceae Recovered from the Environment of a Swine Farrow-to-Finish Operation in the United States"],"prefix":"10.1128","volume":"61","author":[{"given":"Dixie F.","family":"Mollenkopf","sequence":"first","affiliation":[{"name":"Department of Veterinary Preventive Medicine, The Ohio State University College of Veterinary Medicine, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason W.","family":"Stull","sequence":"additional","affiliation":[{"name":"Department of Veterinary Preventive Medicine, The Ohio State University College of Veterinary Medicine, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitria A.","family":"Mathys","sequence":"additional","affiliation":[{"name":"Department of Veterinary Preventive Medicine, The Ohio State University College of Veterinary Medicine, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0738-8453","authenticated-orcid":false,"given":"Andrew S.","family":"Bowman","sequence":"additional","affiliation":[{"name":"Department of Veterinary Preventive Medicine, The Ohio State University College of Veterinary Medicine, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sydnee M.","family":"Feicht","sequence":"additional","affiliation":[{"name":"Department of Veterinary Preventive Medicine, The Ohio State University College of Veterinary Medicine, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan V.","family":"Grooters","sequence":"additional","affiliation":[{"name":"Department of Veterinary Preventive Medicine, The Ohio State University College of Veterinary Medicine, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua B.","family":"Daniels","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Wittum","sequence":"additional","affiliation":[{"name":"Department of Veterinary Preventive Medicine, The Ohio State University College of Veterinary Medicine, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq520"},{"key":"e_1_3_3_3_2","unstructured":"CDC. 2013. 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