{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:53:10Z","timestamp":1790185990309,"version":"4.1.0"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Multiresistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Acinetobacter baumannii<\/jats:named-content>\n            , a common etiologic agent of severe nosocomial infections in compromised hosts, usually harbors\n            <jats:italic>aac(6<\/jats:italic>\n            \u2032\n            <jats:italic>)-Ib.<\/jats:italic>\n            This gene specifies resistance to amikacin and other aminoglycosides, seriously limiting the effectiveness of these antibiotics. An antisense oligodeoxynucleotide (ODN4) that binds to a duplicated sequence on the\n            <jats:italic>aac(6<\/jats:italic>\n            \u2032\n            <jats:italic>)-Ib<\/jats:italic>\n            mRNA, one of the copies overlapping the initiation codon, efficiently inhibited translation\n            <jats:italic>in vitro<\/jats:italic>\n            . An isosequential nuclease-resistant hybrid oligomer composed of 2\u2032,4\u2032-bridged nucleic acid-NC (BNA\n            <jats:sup>NC<\/jats:sup>\n            ) residues and deoxynucleotides (BNA\n            <jats:sup>NC<\/jats:sup>\n            -DNA) conjugated to the permeabilizing peptide (RXR)\n            <jats:sub>4<\/jats:sub>\n            XB (\u201cX\u201d and \u201cB\u201d stand for 6-aminohexanoic acid and \u03b2-alanine, respectively) (CPPBD4) inhibited translation\n            <jats:italic>in vitro<\/jats:italic>\n            at the same levels observed in testing ODN4. Furthermore, CPPBD4 in combination with amikacin inhibited growth of a clinical\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. baumannii<\/jats:named-content>\n            strain harboring\n            <jats:italic>aac(6<\/jats:italic>\n            \u2032\n            <jats:italic>)-Ib<\/jats:italic>\n            in liquid cultures, and when both compounds were used as combination therapy to treat infected\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Galleria mellonella<\/jats:named-content>\n            organisms, survival was comparable to that seen with uninfected controls.\n          <\/jats:p>","DOI":"10.1128\/aac.01304-15","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T02:24:44Z","timestamp":1436840684000},"page":"5798-5803","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Inhibition of AAC(6\u2032)-Ib-Mediated Resistance to Amikacin in Acinetobacter baumannii by an Antisense Peptide-Conjugated 2\u2032,4\u2032-Bridged Nucleic Acid-NC-DNA Hybrid Oligomer"],"prefix":"10.1128","volume":"59","author":[{"given":"Christina","family":"Lopez","sequence":"first","affiliation":[{"name":"Center for Applied Biotechnology Studies, Department of Biological Science, California State University Fullerton, Fullerton, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brock A.","family":"Arivett","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Miami University, Oxford, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis A.","family":"Actis","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Miami University, Oxford, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo E.","family":"Tolmasky","sequence":"additional","affiliation":[{"name":"Center for Applied Biotechnology Studies, Department of Biological Science, California State University Fullerton, Fullerton, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046984"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(88)80233-X"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01250-08"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01464-06"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.09.075"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1089\/154545703322860753"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00709-06"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2014.09.015"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1089\/nat.2012.0362"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-6-24"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2013.05.041"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0047690"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/86753"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.16.8468"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.10.3296-3304.2003"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906529106"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.12755"},{"key":"e_1_3_3_19_2","first-page":"319","volume-title":"Antisense antibacterials: from proof-of-concept to therapeutic perspectives","author":"Bai H","year":"2012","unstructured":"Bai H, Luo X. 2012. 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