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Paired ceftazidime\/avibactam-susceptible\/resistant isolates were obtained before and during ceftazidime\/avibactam treatment. Whole genome sequencing revealed a differential mutation in AmpC (R148W) in the ceftazidime\/avibactam-resistant isolate. Molecular cloning and structural studies confirmed the impact of this substitution, which affects the architecture of the H10 helix, on the evolved resistant phenotype.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.01307-24","type":"journal-article","created":{"date-parts":[[2024,11,6]],"date-time":"2024-11-06T14:01:25Z","timestamp":1730901685000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["<i>In vivo<\/i>\n            emergence of resistance to ceftazidime\/avibactam through modification of chromosomal AmpC \u03b2-lactamase in\n            <i>Klebsiella aerogenes<\/i>"],"prefix":"10.1128","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8270-0259","authenticated-orcid":false,"given":"Salud","family":"Rodr\u00edguez-Pallares","sequence":"first","affiliation":[{"name":"Servicio de Microbiolog\u00eda and Instituto de Investigaci\u00f3n Biom\u00e9dica A Coru\u00f1a 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