{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T16:50:54Z","timestamp":1761929454873},"reference-count":24,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Topical therapy is an attractive approach for the treatment of<jats:italic>L<\/jats:italic><jats:italic>eishmania<\/jats:italic><jats:italic>major<\/jats:italic>cutaneous leishmaniasis (CL). WR279396, an expanded-spectrum aminoglycoside ointment, is now in phase 3 trials. Because the application of a cream is easier than the injection of pentavalent antimony, many patients with CL will likely be treated with WR279396 soon after the onset of a lesion. However, this new therapeutic approach may impair the acquisition of immunity. We evaluated the impact of early topical therapy on acquired immunity in an optimized mouse model of<jats:italic>L. major<\/jats:italic>-induced CL. The efficacy of the WR279396 ointment in this model has been established previously. Acquired immunity was defined as the absence of lesions upon reinoculation of the same parasite isolate at a different skin site. Bioluminescence-based follow-up of luciferase-expressing<jats:italic>L. major<\/jats:italic>loads was also performed. In this model, the control of<jats:italic>L. major<\/jats:italic>loads at the initial inoculation site and the acquisition of immunity are simultaneous (day 22 postinoculation). The clinical and parasitological efficacies of WR279396 applied as early as day 11 postinoculation, i.e., during the<jats:italic>L. major<\/jats:italic>multiplication phase, did not impair the acquisition of immunity to a second<jats:italic>L. major<\/jats:italic>challenge. This is reassuring from the perspective of the wide deployment of WR279396-based therapy in foci where<jats:italic>L. major<\/jats:italic>is endemic.<\/jats:p>","DOI":"10.1128\/aac.01310-09","type":"journal-article","created":{"date-parts":[[2009,12,29]],"date-time":"2009-12-29T01:54:15Z","timestamp":1262051655000},"page":"984-990","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Early Curative Applications of the Aminoglycoside WR279396 on an Experimental<i>Leishmania major<\/i>-Loaded Cutaneous Site Do Not Impair the Acquisition of Immunity"],"prefix":"10.1128","volume":"54","author":[{"given":"Herve\u0301","family":"Lecoeur","sequence":"first","affiliation":[{"name":"Institut Pasteur, De\u0301partement de Parasitologie et Mycologie, Unite\u0301 d'Immunophysiologie et Parasitisme Intracellulaire, 25 Rue du Dr. Roux, Paris 75724 Cedex 15, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre A.","family":"Buffet","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unite\u0301 de Mycologie Me\u0301dicale, and Service de Parasitologie Mycologie, INSERM UMR 945 CHU Ho\u0302pital Pitie\u0301 Salpe\u0302trie\u0300re, 47-83 Boulevard de l'Ho\u0302pital Paris 75651 Cedex 13, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Genevie\u0300ve","family":"Milon","sequence":"additional","affiliation":[{"name":"Institut Pasteur, De\u0301partement de Parasitologie et Mycologie, Unite\u0301 d'Immunophysiologie et Parasitisme Intracellulaire, 25 Rue du Dr. Roux, Paris 75724 Cedex 15, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thierry","family":"Lang","sequence":"additional","affiliation":[{"name":"Institut Pasteur, De\u0301partement de Parasitologie et Mycologie, Unite\u0301 d'Immunophysiologie et Parasitisme Intracellulaire, 25 Rue du Dr. Roux, Paris 75724 Cedex 15, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.2.969"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01152"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000432"},{"key":"e_1_3_2_5_2","unstructured":"298. Abstr. 4th World Congr. Leishmaniasis 2009"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.2307\/3279633"},{"key":"e_1_3_2_7_2","first-page":"301","volume":"7","year":"1970","unstructured":"Bryceson, A. D., R. S. Bray, R. A. Wolstencroft, and D. C. Dumonde. 1970. Immunity in cutaneous leishmaniasis of the guinea-pig. Clin. Exp. Immunol.7:301-341.","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_3_2_8_2","first-page":"503","volume":"121","year":"1994","unstructured":"Buffet, P., E. Caumes, and M. Gentilini. 1994. Treatment of localized cutaneous leishmaniasis. Ann. Dermatol. Venereol.121:503-511. (In French.)","journal-title":"Ann. Dermatol. Venereol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02555-06"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(03)00142-7"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.2307\/3285646"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.777-780.2006"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2004.00468.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"4537","DOI":"10.4049\/jimmunol.157.10.4537","volume":"157","year":"1996","unstructured":"Leclercq, V., M. Lebastard, Y. Belkaid, J. Louis, and G. Milon. 1996. The outcome of the parasitic process initiated by Leishmania infantum in laboratory mice: a tissue-dependent pattern controlled by the Lsh and MHC loci. J. Immunol.157:4537-4545.","journal-title":"J. Immunol."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000001"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20040298"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-9292-6-3"},{"key":"e_1_3_2_18_2","first-page":"337","volume":"10","year":"1972","unstructured":"Preston, P. M., R. L. Carter, E. Leuchars, A. J. Davies, and D. C. Dumonde. 1972. Experimental cutaneous leishmaniasis. 3. Effects of thymectomy on the course of infection of CBA mice with Leishmania tropica. Clin. Exp. Immunol.10:337-357.","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_3_2_19_2","first-page":"126","volume":"23","year":"1976","unstructured":"Preston, P. M., and D. C. Dumonde. 1976. Experimental cutaneous leishmaniasis. 5. Protective immunity in subclinical and self-healing infection in mouse. Clin. Exp. Immunol.23:126-138.","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(07)70209-8"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2002.66.147"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.2001.4656"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(87)90115-3"},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"4891","DOI":"10.4049\/jimmunol.151.9.4891","volume":"151","year":"1993","unstructured":"Sunderkotter, C., M. Kunz, K. Steinbrink, G. Meinardus-Hager, M. Goebeler, H. Bildau, and C. Sorg. 1993. Resistance of mice to experimental leishmaniasis is associated with more rapid appearance of mature macrophages in vitro and in vivo. J. Immunol.151:4891-4901.","journal-title":"J. Immunol."},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"1614","DOI":"10.4049\/jimmunol.126.4.1614","volume":"126","year":"1981","unstructured":"Taswell, C. 1981. Limiting dilution assays for the determination of immunocompetent cell frequencies. I. Data analysis. J. Immunol.126:1614-1619.","journal-title":"J. Immunol."}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01310-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01310-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,29]],"date-time":"2023-05-29T09:15:03Z","timestamp":1685351703000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01310-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3]]},"references-count":24,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2010,3]]}},"alternative-id":["10.1128\/AAC.01310-09"],"URL":"https:\/\/doi.org\/10.1128\/aac.01310-09","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3]]},"assertion":[{"value":"2009-09-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-12-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}