{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T16:22:16Z","timestamp":1791649336155,"version":"4.3.4"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Agriculture and Food Research Initiative Competitive Grant","award":["2021-08162"],"award-info":[{"award-number":["2021-08162"]}]},{"name":"NIH\/NIAID","award":["AI162944"],"award-info":[{"award-number":["AI162944"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,2,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The gut environment includes an abundance of chemicals emanating from the host and the microbiota. To colonize animals,\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      uses gut chemicals as locational cues to ensure expression of energy-intensive virulence factors only when their production is necessary.\n                      <jats:italic toggle=\"yes\">cis<\/jats:italic>\n                      -2-hexadecenoic acid (c2HDA), a member of the diffusible signal factor family of quorum-sensing signals, potently represses virulence-gene expression by\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      . Here, we report the construction and use of a recombinant bacteriophage that can establish lysogeny within\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      and induce it to produce c2HDA, thus repressing functions essential to its own virulence. We engineered the temperate phage P22 to favor lysogeny through transposon mutagenesis of the\n                      <jats:italic toggle=\"yes\">sieB-esc<\/jats:italic>\n                      region and caused it to produce c2HDA by introduction of\n                      <jats:italic toggle=\"yes\">rpfF<\/jats:italic>\n                      of\n                      <jats:italic toggle=\"yes\">Cronobacter turicensis<\/jats:italic>\n                      , encoding the dehydratase\/thioesterase required for c2HDA synthesis. We found that\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      harboring the lysogenic phage carrying\n                      <jats:italic toggle=\"yes\">rpfF<\/jats:italic>\n                      produced c2HDA and repressed invasion-gene expression both endogenously and exogenously through secretion into the surrounding medium. Consequently, this phage reduced\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      invasion of epithelial cells by over 100-fold. We further found that both wild-type and c2HDA-producing phage administered orally to mice reduced\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      colonization of the gut, but that the phage carrying\n                      <jats:italic toggle=\"yes\">rpfF<\/jats:italic>\n                      reduced gut inflammation more than did the phage without this gene. Collectively, our data show that a recombinant phage can be used as the vehicle for cytoplasmic delivery of c2HDA, thus providing a targeted means to manipulate\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      colonization of the gut.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01341-25","type":"journal-article","created":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T14:00:15Z","timestamp":1766498415000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["A recombinant lysogenic bacteriophage inhibits\n                    <i>Salmonella<\/i>\n                    virulence"],"prefix":"10.1128","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1804-244X","authenticated-orcid":true,"given":"Erick Maosa","family":"Bosire","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Population Medicine and Diagnostic Sciences, Cornell University","place":["Ithaca, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mudasir Ali","family":"Rather","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Population Medicine and Diagnostic Sciences, Cornell University","place":["Ithaca, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine E.","family":"Bell","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Population Medicine and Diagnostic Sciences, Cornell University","place":["Ithaca, 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