{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:52:16Z","timestamp":1785433936655,"version":"3.56.0"},"reference-count":76,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Pengfei Cui","award":["31902421"],"award-info":[{"award-number":["31902421"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,2,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The escalating threat posed by multidrug-resistant (MDR) Gram-negative \u201csuperbugs\u201d has intensified. Short antimicrobial peptides (SAMPs) have emerged as promising therapeutics with sustained potency and cost-effectiveness against drug-resistant infections. Here, we report a family of 15-residue SAMPs derived through modifying related amino acids of Kassporin-KS1 (FA), utilizing database-filtering technology to identify the most probable structural parameters related to Gram-negative bacteria. Most SAMPs exhibit sub-\u03bcM antimicrobial activity with reliable stability and low toxicity. Notably, KR and RK demonstrate significant efficacy in combating biofilms and sepsis infections\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      . Furthermore, the acquisition of resistance by strains to SAMPs was not observed, primarily due to the multimodal antimicrobial mechanisms of SAMPs. We revealed that the multimodal mechanisms of SAMPs encompass unregulated membrane destabilization, induction of apoptotic-like death pathway, and interference with normal physiological processes. Overall, the rational design strategies proposed herein can be implemented to develop potent antimicrobial agents targeting MDR bacteria.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01343-25","type":"journal-article","created":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T18:55:05Z","timestamp":1766516105000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["A short antimicrobial peptides family demonstrates efficacy to infection via a multimodal mechanism of action"],"prefix":"10.1128","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7722-4369","authenticated-orcid":true,"given":"Yifan","family":"Liu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04rdtx186","id-type":"ROR","asserted-by":"publisher"}],"name":"Lab of Environmental Health and Ecological Engineering, College of Marine Life Science, Ocean University of 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