{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T07:20:20Z","timestamp":1787728820253,"version":"build-2784847793"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81371840"],"award-info":[{"award-number":["81371840"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            This study investigated the antischistosomiasis liver fibrosis effects of chlorogenic acid (CGA) on interleukin 13 (IL-13)\/microRNA-21 (miR-21)\/Smad7 signaling interactions in the hepatic stellate LX2 cell line and schistosome-infected mice. The transfection was based on the ability of the GV273\u2013miR-21\u2013enhanced green fluorescent protein (EGFP) and GV369\u2013miR-21\u2013EGFP lentiviral system to up- or downregulate the miR-21 gene in LX2 cells. The mRNA expression of miR-21, Smad7, and connective tissue growth factor (CTGF) and the protein expression of Smad7, CTGF, Smad1, phosphor-Smad1 (p-Smad1), Smad2, p-Smad2, Smad2\/3, p-Smad2\/3, transforming growth factor \u03b2 (TGF-\u03b2) receptor I, and \u03b1-smooth muscle actin (\u03b1-SMA) was assayed. Pathological manifestation of hepatic tissue was assessed for the degree of liver fibrosis in animals. The results showed that CGA could inhibit the mRNA expression of miR-21, promote Smad7, and inhibit CTGF mRNA expression. Meanwhile, CGA could significantly lower the protein levels of CTGF, p-Smad1, p-Smad2, p-Smad2\/3, TGF-\u03b2 receptor I, and \u03b1-SMA and elevate the Smad7 protein level.\n            <jats:italic>In vivo<\/jats:italic>\n            , with treatment with CGA, the signaling molecules of IL-13\/miR-21\/Smad7 interactions were markedly regulated. CGA could also reduce the degree of liver fibrosis in pathological manifestations. In conclusion, CGA could inhibit schistosomiasis-induced hepatic fibrosis through IL-13\/miR-21\/Smad7 signaling interactions in LX2 cells and schistosome-infected mice and might serve as an antifibrosis agent for treating schistosomiasis liver fibrosis.\n          <\/jats:p>","DOI":"10.1128\/aac.01347-16","type":"journal-article","created":{"date-parts":[[2016,11,22]],"date-time":"2016-11-22T03:20:35Z","timestamp":1479784835000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Antischistosomiasis Liver Fibrosis Effects of Chlorogenic Acid through IL-13\/miR-21\/Smad7 Signaling Interactions\n            <i>In Vivo<\/i>\n            and\n            <i>In Vitro<\/i>"],"prefix":"10.1128","volume":"61","author":[{"given":"Yao","family":"Wang","sequence":"first","affiliation":[{"name":"School of Clinical Medicine, Hubei University of Chinese Medicine, Wuhan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Hepatology, Hubei Provincial Hospital of Chinese Medicine, Wuhan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Xue","sequence":"additional","affiliation":[{"name":"Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Luo","sequence":"additional","affiliation":[{"name":"School of Clinical Medicine, Hubei University of Chinese Medicine, Wuhan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Life Science, Hubei University, Wuhan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Fei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Clinical Medicine, Hubei University of Chinese Medicine, Wuhan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Ling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000807"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.4137\/IDRT.S24342"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)61949-2"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2013.07.010"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201208390"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-012-3151-z"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003260"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v16.i41.5211"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.54.031902.134938"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.4958"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf010514x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.1722"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2015.01.036"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2008.10.006"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.63.1295"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/hr.2011.195"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf062556x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0944-7113(00)80034-3"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.29.2236"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf8018535"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2012.04.010"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2009.09.026"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2012.10.025"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2016.03.002"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2013.12.009"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201141869"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.27671"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.27748"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2007.05.015"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-3231.2008.01826.x"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-011110-130246"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2001.25350"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22820"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-012-0376-8"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-011-1052-6"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/jhbp.126"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/jp.2014.58"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/ecam\/nem165"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2010.10.051"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-014-8947-7"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2016.03.001"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-230X-13-79"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182016001128"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1177\/039463201302600108"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.6466"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1177\/039463201202500128"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1177\/039463201102400309"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1177\/1721727X1301100115"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1080\/08820130802111589"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-015-0208-y"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-015-0744-6"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01347-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01347-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T20:38:20Z","timestamp":1645475900000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01347-16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2]]},"references-count":52,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,2]]}},"alternative-id":["10.1128\/AAC.01347-16"],"URL":"https:\/\/doi.org\/10.1128\/aac.01347-16","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2]]},"assertion":[{"value":"2016-06-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-11-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-01-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01347-16"}}