{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:58:54Z","timestamp":1791097134321,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2009,5,1]],"date-time":"2009-05-01T00:00:00Z","timestamp":1241136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Carbapenem-resistant\n            <jats:italic>Klebsiella<\/jats:italic>\n            strains carrying\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            carbapenemases (KPC) are endemic to New York City and are spreading across the United States and internationally. Recent studies have indicated that the KPC structural gene is located on a 10-kb plasmid-borne element designated Tn\n            <jats:italic>4401<\/jats:italic>\n            . Fourteen\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            strains and one\n            <jats:italic>Klebsiella oxytoca<\/jats:italic>\n            strain isolated at a New York City hospital in 2005 carrying either\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC-2<\/jats:sub>\n            or\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC-3<\/jats:sub>\n            were examined for isoforms of Tn\n            <jats:italic>4401<\/jats:italic>\n            . Ten of the\n            <jats:italic>Klebsiella<\/jats:italic>\n            strains contained a 100-bp deletion in Tn\n            <jats:italic>4401<\/jats:italic>\n            , corresponding to the Tn\n            <jats:italic>4401<\/jats:italic>\n            a isoform. The presence of this deletion adjacent to the upstream promoter region of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC<\/jats:sub>\n            in Tn\n            <jats:italic>4401<\/jats:italic>\n            a resulted in a different \u221235 promoter sequence of TGGAGA than that of CTGATT present in isoform Tn\n            <jats:italic>4401<\/jats:italic>\n            b. Complete sequencing of one plasmid carrying\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC<\/jats:sub>\n            from each of three nonclonal isolates indicated the presence of genes encoding other types of antibiotic resistance determinants. The 70.6-kb plasmid from\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            strain S9 carrying\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC-2<\/jats:sub>\n            revealed two identical copies of Tn\n            <jats:italic>4401<\/jats:italic>\n            b inserted in an inverse fashion, but in this case, one of the elements disrupted a group II self-splicing intron. In\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            strain S15, the Tn\n            <jats:italic>4401<\/jats:italic>\n            a element carrying\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC-2<\/jats:sub>\n            was found on both a large 120-kb plasmid and a smaller 24-kb plasmid. Pulsed-field gel electrophoresis results indicate that the isolates studied represent a heterogeneous group composed of unrelated as well as closely related\n            <jats:italic>Klebsiella<\/jats:italic>\n            strains. Our results suggest that endemic KPC-positive\n            <jats:italic>Klebsiella<\/jats:italic>\n            strains constitute a generally nonclonal population comprised of various alleles of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC<\/jats:sub>\n            on several distinct plasmid genetic backgrounds. This study increases our understanding of the genetic composition of the evolving and expanding role of KPC-producing, healthcare-associated, gram-negative pathogens.\n          <\/jats:p>","DOI":"10.1128\/aac.01355-08","type":"journal-article","created":{"date-parts":[[2009,3,3]],"date-time":"2009-03-03T02:54:31Z","timestamp":1236048871000},"page":"1998-2004","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":141,"title":["Genetic Organization of Transposase Regions Surrounding\n            <i>bla<\/i>\n            <sub>KPC<\/sub>\n            Carbapenemase Genes on Plasmids from\n            <i>Klebsiella<\/i>\n            Strains Isolated in a New York City Hospital"],"prefix":"10.1128","volume":"53","author":[{"given":"Thomas D.","family":"Gootz","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Kay","family":"Lescoe","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fadia","family":"Dib-Hajj","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian A.","family":"Dougherty","sequence":"additional","affiliation":[{"name":"Molecular Biology, Pfizer Global Research and Development, Groton, Connecticut 06340"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"He","sequence":"additional","affiliation":[{"name":"Molecular Biology, Pfizer Global Research and Development, Groton, Connecticut 06340"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phyllis","family":"Della-Latta","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Service and the Department of Pathology, New York-Presbyterian Hospital, Columbia University Medical Center, New York, New York 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard C.","family":"Huard","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Service and the Department of Pathology, New York-Presbyterian Hospital, Columbia University Medical Center, New York, New York 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.11.4760-4762.2005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00015-07"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/421495"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.165.12.1430"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki175"},{"key":"e_1_3_2_7_2","unstructured":"CLSI M100-S15. 2005"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01180-07"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.07.004"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2005.11.006"},{"key":"e_1_3_2_11_2","unstructured":"Abstr. 17th Eur. Congr. Clin. Microbiol. Infect. Dis. 2007"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.11.4438-4440.2004"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00285-06"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi700300u"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/MRM.0b013e3282f0b8b2"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm129"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00299-07"},{"key":"e_1_3_2_18_2","unstructured":"P-2004. Abstr. 18th Eur. Congr. Clin. Microbiol. Infect. Dis. 2008"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.4.1297-1300.2003"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01451-07"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.10.4423-4424.2005"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00438-06"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2002.00401.x"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00163-08"},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.gene.2005.09.004","volume":"366","year":"2006","unstructured":"Rodr\u00edguez-Trelles, F., R. Tarrio, and F. Ayala. 2006. Models of the spliceosomal intron proliferation in the FACE of widespread ectopic expression. Gene366:201-208.","journal-title":"Gene"},{"key":"e_1_3_2_26_2","first-page":"2141","volume":"37","year":"1999","unstructured":"Saito, M., A. Ukmeda, and S.-I. Yoshida. 1999. Subtyping of Haemophilus influenzae strains by pulsed-field gel electrophoresis. J. Clin. Microbiol.37:2141-2147.","journal-title":"J. Clin. Microbiol."},{"key":"e_1_3_2_27_2","unstructured":"Molecular cloning: a laboratory manual 1989"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.33.9.2233-2239.1995"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1208.060291"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01405-06"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00186-06"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01053-06"},{"key":"e_1_3_2_33_2","unstructured":"P-1629. Abstr. 17th Congr. Clin. Microbiol. Infect. Dis. 2007"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2006.11.020"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.12.4793-4799.2004"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.4.1151-1161.2001"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.12.3881-3889.2003"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl541"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01355-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01355-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T20:23:35Z","timestamp":1645475015000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01355-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,5]]},"references-count":37,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2009,5]]}},"alternative-id":["10.1128\/AAC.01355-08"],"URL":"https:\/\/doi.org\/10.1128\/aac.01355-08","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,5]]},"assertion":[{"value":"2008-10-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-02-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}