{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:34:53Z","timestamp":1785429293924,"version":"3.56.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82072297, 82572607"],"award-info":[{"award-number":["82072297, 82572607"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      While malaria parasites rely on labile Fe\n                      <jats:sup>2+<\/jats:sup>\n                      pools for survival, excess Fe\n                      <jats:sup>2+<\/jats:sup>\n                      acts as a Fenton reagent, inducing cytotoxicity via reactive oxygen species and membrane disruption, highlighting iron homeostasis as a key therapeutic vulnerability. To test the feasibility of iron ions in inhibiting\n                      <jats:italic toggle=\"yes\">Plasmodium<\/jats:italic>\n                      parasites, we developed Fe\n                      <jats:sup>2+<\/jats:sup>\n                      -loaded polydopamine nanoparticles (PDA@Fe\/P) that exploit the parasite\u2019s iron-dependent vulnerabilities through dual mechanisms: (i) sustained Fe\n                      <jats:sup>2+<\/jats:sup>\n                      release triggers Fenton reactions, generating cytotoxic hydroxyl radicals that overwhelm antioxidant defenses, and (ii) restoration of artemisinin (ART) activation in resistant parasites by supplementing the diminished Fe\n                      <jats:sup>2+<\/jats:sup>\n                      pool.\n                      <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                      testing against five\n                      <jats:italic toggle=\"yes\">P. falciparum<\/jats:italic>\n                      strains (including chloroquine- and ART-resistant variants) demonstrated potent, antimalarial activity, with efficacy 20-fold higher than free FeCl\n                      <jats:sub>2<\/jats:sub>\n                      due to enhanced solubility and controlled release. While\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      studies in\n                      <jats:italic toggle=\"yes\">P. berghei<\/jats:italic>\n                      -infected mice showed transient parasite suppression without toxicity, the relatively high IC\n                      <jats:sub>50<\/jats:sub>\n                      precludes standalone use. Crucially, PDA@Fe\/P enhanced the activity of ART against\n                      <jats:italic toggle=\"yes\">P. falciparum<\/jats:italic>\n                      strain with partial ART resistance conferred by Kelch13 mutation, by counteracting mutation-induced impairments in hemoglobin endocytosis and heme bioavailability\u2014key determinants of ART activation. Analysis of lipid peroxidation levels revealed that Fe\n                      <jats:sup>2+<\/jats:sup>\n                      delivered via PDA@Fe\/P amplifies oxidative stress responses in resistant parasites, indicating its ability to enhance the sensitivity of ART-resistant strains to ART. Our findings establish iron-based delivery strategies as a promising approach to potentiate existing antimalarials and combat resistance through the targeted disruption of redox homeostasis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01390-25","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T14:00:38Z","timestamp":1771596038000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["A Fe-incorporated bioreactor platform exhibiting antimalarial activity and enhanced response to artemisinin"],"prefix":"10.1128","volume":"70","author":[{"given":"Yinyue","family":"Li","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Basic Medical Sciences, Faculty of Medicine, Yangzhou University","place":["Yangzhou, 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