{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T10:57:17Z","timestamp":1790506637527,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2011,2,1]],"date-time":"2011-02-01T00:00:00Z","timestamp":1296518400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Alveolar echinococcosis (AE) is caused by the metacestode stage of the fox tapeworm<jats:italic>Echinococcus multilocularis<\/jats:italic>and causes severe disease in the human liver, and occasionally in other organs, that is fatal when treatment is unsuccessful. The present chemotherapy against AE is based on mebendazole and albendazole. Albendazole treatment has been found to be ineffective in some instances, is parasitostatic rather than parasiticidal, and usually involves the lifelong uptake of large doses of drugs. Thus, new treatment options are urgently needed. In this study we investigated the<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>efficacy of mefloquine against<jats:italic>E. multilocularis<\/jats:italic>metacestodes. Treatment using mefloquine (20 \u03bcM) against<jats:italic>in vitro<\/jats:italic>cultures of metacestodes resulted in rapid and complete detachment of large parts of the germinal layer from the inner surface of the laminated layer within a few hours. The<jats:italic>in vitro<\/jats:italic>activity of mefloquine was dependent on the dosage.<jats:italic>In vitro<\/jats:italic>culture of metacestodes in the presence of 24 \u03bcM mefloquine for a period of 10 days was parasiticidal, as determined by murine bioassays, while treatment with 12 \u03bcM was not. Oral application of mefloquine (25 mg\/kg of body weight administered twice a week for a period of 8 weeks) in<jats:italic>E. multilocularis<\/jats:italic>-infected mice was ineffective in achieving any reduction of parasite weight, whereas treatment with albendazole (200 mg\/kg\/day) was highly effective. However, when the same mefloquine dosage was applied intraperitoneally, the reduction in parasite weight was similar to the reduction seen with oral albendazole application. Combined application of both drugs did not increase the treatment efficacy. In conclusion, mefloquine represents an interesting drug candidate for the treatment of AE, and these results should be followed up in appropriate<jats:italic>in vivo<\/jats:italic>studies.<\/jats:p>","DOI":"10.1128\/aac.01392-10","type":"journal-article","created":{"date-parts":[[2010,12,7]],"date-time":"2010-12-07T06:29:34Z","timestamp":1291703374000},"page":"713-721","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["<i>In Vitro<\/i>and<i>In Vivo<\/i>Efficacies of Mefloquine-Based Treatment against Alveolar Echinococcosis"],"prefix":"10.1128","volume":"55","author":[{"given":"Tatiana","family":"Ku\u0308ster","sequence":"first","affiliation":[{"name":"Institute of Parasitology, Vetsuisse Faculty, University of Berne, La\u0308nggassstrasse 122, CH-3012 Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Britta","family":"Stadelmann","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Vetsuisse Faculty, University of Berne, La\u0308nggassstrasse 122, CH-3012 Berne, Switzerland"},{"name":"Institute of Cell and Molecular Biology, Biomolecular Centre, University of Uppsala, Husargatan 3, SE-75124 Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corina","family":"Hermann","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Vetsuisse Faculty, University of Berne, La\u0308nggassstrasse 122, CH-3012 Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Scholl","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Vetsuisse Faculty, University of Berne, La\u0308nggassstrasse 122, CH-3012 Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Keiser","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology and Infection Biology, Swiss Tropical Institute, Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Hemphill","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Vetsuisse Faculty, University of Berne, La\u0308nggassstrasse 122, CH-3012 Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1159\/000109767"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705721"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.1992.tb04081.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.1111\/j.2042-7158.1997.tb06047.x","volume":"49","year":"1997","unstructured":"Baudry, S., et al. 1997. 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