{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T07:47:02Z","timestamp":1790236022771,"version":"4.1.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n Multidrug-resistant (MDR)\n Acinetobacter<\/jats:italic>\n spp. have emerged as a threat to public health. We investigated the various genes involved in resistance to fluoroquinolones, aminoglycosides, cephalosporins, and carbapenems in 75 clinical\n Acinetobacter<\/jats:italic>\n isolates from a Taiwanese hospital. All isolates were tested for the\n gyrA<\/jats:italic>\n mutations, the presence of integrons,\n bla<\/jats:italic>\n AmpC<\/jats:sub>\n , and carbapenem resistance genes. The Ser83Leu mutation in GyrA accounted for fluoroquinolone resistance. The presence of integrons containing aminoglycoside-modifying enzymes was associated with resistance to gentamicin and tobramycin but not with resistance to amikacin. The presence of an IS\n Aba1<\/jats:italic>\n element upstream of\n bla<\/jats:italic>\n AmpC<\/jats:sub>\n was correlated with cephalosporin resistance. Although most\n Acinetobacter<\/jats:italic>\n baumannii<\/jats:italic>\n isolates with IS\n Aba1<\/jats:italic>\n -\n bla<\/jats:italic>\n OXA-51-<\/jats:sub>\n like<\/jats:sub>\n were resistant to carbapenems, several isolates remained susceptible to carbapenems. Transformation by the introduction of IS\n Aba1<\/jats:italic>\n -\n bla<\/jats:italic>\n OXA-23<\/jats:sub>\n or IS\n Aba1<\/jats:italic>\n -\n bla<\/jats:italic>\n OXA-66<\/jats:sub>\n into\n A. baumannii<\/jats:italic>\n ATCC 15151 (CIP 70.10), resulting in the overexpression of OXA-23 or OXA-66, respectively, suggested the role of the IS\n Aba1<\/jats:italic>\n element as a strong promoter. The two transformants showed significantly increased resistance to piperacillin-tazobactam, imipenem, and meropenem. The cefepime resistance conferred by IS\n Aba1<\/jats:italic>\n -\n bla<\/jats:italic>\n OXA-23<\/jats:sub>\n and the impact of IS\n Aba1<\/jats:italic>\n -\n bla<\/jats:italic>\n OXA-66<\/jats:sub>\n on carbapenem resistance in\n A. baumannii<\/jats:italic>\n are reported here for the first time. Continuous surveillance of antibiotic resistance genes in MDR\n Acinetobacter<\/jats:italic>\n spp. and elucidation of their antibiotic resistance mechanisms are crucial for the development of therapy regimens and for the prevention of further dissemination of these antibiotic resistance genes.\n <\/jats:p>","DOI":"10.1128\/aac.01398-09","type":"journal-article","created":{"date-parts":[[2010,3,2]],"date-time":"2010-03-02T04:54:26Z","timestamp":1267505666000},"page":"2078-2084","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Genetic Basis of Multidrug Resistance in\n Acinetobacter<\/i>\n Clinical Isolates in Taiwan"],"prefix":"10.1128","volume":"54","author":[{"given":"Yu-Chi","family":"Lin","sequence":"first","affiliation":[{"name":"Research and Diagnostic Center, Centers for Disease 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